This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arenzana-Seisdedos, F Articles by Virelizier, J L Search for Related Content PubMed PubMed Citation Articles by Arenzana-Seisdedos, F Articles by Virelizier, J L

Phosphatidylcholine hydrolysis activates NF-kappa B and increases human immunodeficiency virus replication in human monocytes and T lymphocytes.

Unite d'Immunologie Virale, Institut Pasteur, Paris, France.

ABSTRACT

We have tested whether breakdown of phosphatidylcholine (PC) initiated by exogenous addition of a PC-specific phospholipase C (PC-PLC) from Bacillus cereus or by endogenous overexpression of PC-PLC induces functional activation of NF-kappa B and increases human immunodeficiency virus (HIV) enhancer activity. PC-PLC-activated hydrolysis of PC was found to induce bona fide p50/p65 NF-kappa B binding activity in three different cell lines of human or murine origin. No significant changes in the turnover of other cellular phospholipids were detected in PC-PLC-treated cells. Induction of NF-kappa B by PC-PLC did not depend on de novo synthesis of proteins or autocrine secretion of either tumor necrosis factor or interleukin 1. In human monocytic and lymphoblastoid T-cell lines, induction of NF-kappa B by PC-PLC resulted in clear induction of luciferase expression vectors placed under the control of synthetic kappa B enhancers or wild type, but not kappa B-mutated, HIV long terminal repeat constructs. HIV replication was increased by PC-PLC in chronically infected monocytes and T lymphocytes. NF-kappa B activation promoted by addition of exogenous PC-PLC correlated with an intense production of diacylglycerol. However, addition of a phosphatidylinositol-specific PLC from B. cereus also induced diacylglycerol but did not activate kappa B enhancer-directed vectors. PC-PLC-induced NF-kappa B activation could not be blocked by a specific inhibitor of phorbol ester-inducible protein kinases C. These results indicate that a cellular transduction pathway, dependent on specific PC breakdown, is functional in T lymphocytes and monocytes and may be used by various transmembrane receptors to activate HIV transcription through NF-kappa B-dependent induction of the HIV enhancer.

This article has been cited by other articles:

• Fantuzzi, L., Spadaro, F., Purificato, C., Cecchetti, S., Podo, F., Belardelli, F., Gessani, S., Ramoni, C. (2008). Phosphatidylcholine-specific phospholipase C activation is required for CCR5-dependent, NF-kB-driven CCL2 secretion elicited in response to HIV-1 gp120 in human primary macrophages. Blood 111: 3355-3363 [Abstract] [Full Text]  
• Hidalgo-Estevez, A. M., Gonzalez, E., Punzon, C., Fresno, M. (2006). Human immunodeficiency virus type 1 Tat increases cooperation between AP-1 and NFAT transcription factors in T cells. J. Gen. Virol. 87: 1603-1612 [Abstract] [Full Text]  
• Tomlinson, M. G., Kane, L. P., Su, J., Kadlecek, T. A., Mollenauer, M. N., Weiss, A. (2004). Expression and Function of Tec, Itk, and Btk in Lymphocytes: Evidence for a Unique Role for Tec. Mol. Cell. Biol. 24: 2455-2466 [Abstract] [Full Text]  
• D'Souza, B. N., Edelstein, L. C., Pegman, P. M., Smith, S. M., Loughran, S. T., Clarke, A., Mehl, A., Rowe, M., Gelinas, C., Walls, D. (2004). Nuclear Factor {kappa}B-Dependent Activation of the Antiapoptotic bfl-1 Gene by the Epstein-Barr Virus Latent Membrane Protein 1 and Activated CD40 Receptor. J. Virol. 78: 1800-1816 [Abstract] [Full Text]  
• Prince, S., Keating, S., Fielding, C., Brennan, P., Floettmann, E., Rowe, M. (2003). Latent Membrane Protein 1 Inhibits Epstein-Barr Virus Lytic Cycle Induction and Progress via Different Mechanisms. J. Virol. 77: 5000-5007 [Abstract] [Full Text]  
• Keating, S., Prince, S., Jones, M., Rowe, M. (2002). The Lytic Cycle of Epstein-Barr Virus Is Associated with Decreased Expression of Cell Surface Major Histocompatibility Complex Class I and Class II Molecules. J. Virol. 76: 8179-8188 [Abstract] [Full Text]  
• Fielding, C. A., Sandvej, K., Mehl, A., Brennan, P., Jones, M., Rowe, M. (2001). Epstein-Barr Virus LMP-1 Natural Sequence Variants Differ in Their Potential To Activate Cellular Signaling Pathways. J. Virol. 75: 9129-9141 [Abstract] [Full Text]  
• Gonzalez, E., Punzon, C., Gonzalez, M., Fresno, M. (2001). HIV-1 Tat Inhibits IL-2 Gene Transcription Through Qualitative and Quantitative Alterations of the Cooperative Rel/AP1 Complex Bound to the CD28RE/AP1 Composite Element of the IL-2 Promoter. J. Immunol. 166: 4560-4569 [Abstract] [Full Text]  
• Algarte, M., Kwon, H., Genin, P., Hiscott, J. (1999). Identification by In Vivo Genomic Footprinting of a Transcriptional Switch Containing NF-kappa B and Sp1 That Regulates the Ikappa Balpha Promoter. Mol. Cell. Biol. 19: 6140-6153 [Abstract] [Full Text]  
• Gil, J., Alcami, J., Esteban, M. (1999). Induction of Apoptosis by Double-Stranded-RNA-Dependent Protein Kinase (PKR) Involves the alpha  Subunit of Eukaryotic Translation Initiation Factor 2 and NF-kappa B. Mol. Cell. Biol. 19: 4653-4663 [Abstract] [Full Text]  
• Rodriguez, M. S., Thompson, J., Hay, R. T., Dargemont, C. (1999). Nuclear Retention of Ikappa Balpha Protects It from Signal-induced Degradation and Inhibits Nuclear Factor kappa B Transcriptional Activation. J. Biol. Chem. 274: 9108-9115 [Abstract] [Full Text]  
• Kroll, M., Margottin, F., Kohl, A., Renard, P., Durand, H., Concordet, J.-P., Bachelerie, F., Arenzana-Seisdedos, F., Benarous, R. (1999). Inducible Degradation of Ikappa Balpha by the Proteasome Requires Interaction with the F-box Protein h-beta TrCP. J. Biol. Chem. 274: 7941-7945 [Abstract] [Full Text]  
• Henriquez, N. V., Floettmann, E., Salmon, M., Rowe, M., Rickinson, A. B. (1999). Differential Responses to CD40 Ligation Among Burkitt Lymphoma Lines That Are Uniformly Responsive to Epstein-Barr Virus Latent Membrane Protein 1. J. Immunol. 162: 3298-3307 [Abstract] [Full Text]  
• Puls, A, Eliopoulos, A., Nobes, C., Bridges, T, Young, L., Hall, A (1999). Activation of the small GTPase Cdc42 by the inflammatory cytokines TNF(alpha) and IL-1, and by the Epstein-Barr virus transforming protein LMP1. J. Cell Sci. 112: 2983-2992 [Abstract]  
• Li, Y., Maher, P., Schubert, D. (1998). Phosphatidylcholine-specific phospholipase C regulates glutamate-induced nerve cell death. Proc. Natl. Acad. Sci. USA 95: 7748-7753 [Abstract] [Full Text]  
• Johnson, R. J., Stack, M., Hazlewood, S. A., Jones, M., Blackmore, C. G., Hu, L.-F., Rowe, M. (1998). The 30-Base-Pair Deletion in Chinese Variants of the Epstein-Barr Virus LMP1 Gene Is Not the Major Effector of Functional Differences between Variant LMP1 Genes in Human Lymphocytes. J. Virol. 72: 4038-4048 [Abstract] [Full Text]  
• Carter, A. B., Monick, M. M., Hunninghake, G. W. (1998). Lipopolysaccharide-induced NF-kappa B Activation and Cytokine Release in Human Alveolar Macrophages Is PKC-independent and TK- and PC-PLC-dependent. Am. J. Respir. Cell Mol. Bio. 18: 384-391 [Abstract] [Full Text]  
• Hauf, N., Goebel, W., Fiedler, F., Sokolovic, Z., Kuhn, M. (1997). Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF-kappa B (RelA/p50) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by Ikappa Balpha and Ikappa Bbeta  degradation. Proc. Natl. Acad. Sci. USA 94: 9394-9399 [Abstract] [Full Text]  
• Arenzana-Seisdedos, F, Turpin, P, Rodriguez, M, Thomas, D, Hay, R., Virelizier, J., Dargemont, C (1997). Nuclear localization of I kappa B alpha promotes active transport of NF-kappa B from the nucleus to the cytoplasm. J. Cell Sci. 110: 369-378 [Abstract]  
• Legrand-Poels, S., Bours, V., Piret, B., Pflaum, M., Epe, B., Rentier, B., Piette, J. (1995). Transcription Factor NF-kappaB Is Activated by Photosensitization Generating Oxidative DNA Damages. J. Biol. Chem. 270: 6925-6934 [Abstract] [Full Text]  
• Mehl, A. M., Floettmann, J. E., Jones, M., Brennan, P., Rowe, M. (2001). Characterization of Intercellular Adhesion Molecule-1 Regulation by Epstein-Barr Virus-encoded Latent Membrane Protein-1 Identifies Pathways That Cooperate with Nuclear Factor kappa B to Activate Transcription. J. Biol. Chem. 276: 984-992 [Abstract] [Full Text]  
• Brennan, P., Floettmann, J. E., Mehl, A., Jones, M., Rowe, M. (2001). Mechanism of Action of a Novel Latent Membrane Protein-1 Dominant Negative. J. Biol. Chem. 276: 1195-1203 [Abstract] [Full Text]  
• de Gregorio, R., Iniguez, M. A., Fresno, M., Alemany, S. (2001). Cot Kinase Induces Cyclooxygenase-2 Expression in T Cells through Activation of the Nuclear Factor of Activated T Cells. J. Biol. Chem. 276: 27003-27009 [Abstract] [Full Text]