This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Craven, M G Articles by Nuss, D L Search for Related Content PubMed PubMed Citation Articles by Craven, M G Articles by Nuss, D L

 Previous Article  |  Next Article 

J Virol. 1993 November; 67(11): 6513-6521

Papain-like protease p29 as a symptom determinant encoded by a hypovirulence-associated virus of the chestnut blight fungus.

Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.

ABSTRACT

Viral double-stranded RNAs (dsRNAs) responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus, Cryphonectria parasitica, profoundly influence a range of host functions in addition to virulence. The 5'-proximal open reading frame, A, of the prototypical hypovirulence-associated viral dsRNA, L-dsRNA, present in hypovirulent strain EP713, was recently shown by DNA-mediated transformation analysis to suppress fungal sporulation, pigmentation, and accumulation of the enzyme laccase (G. H. Choi and D. L. Nuss, EMBO J. 11:473-477, 1992). We mapped this suppressive activity to the autocatalytic papain-like protease, p29, present within the amino-terminal portion of open reading frame A-encoded polyprotein p69. Mutational analysis revealed that the ability of p29 to alter fungal phenotype is dependent upon release from the polyprotein precursor but is independent of intrinsic proteolytic activity. Deletion of the p29-coding domain within the context of an infectious L-dsRNA cDNA clone resulted in a replication-competent viral dsRNA that exhibited intermediate suppressive activity while retaining the ability to confer hypovirulence. Thus, p29 is necessary but not sufficient for the level of virus-mediated suppression of fungal pigmentation, sporulation, and laccase accumulation observed for wild-type hypovirulent strain EP713 and is nonessential for viral RNA replication and virulence attenuation. These results also illustrate the feasibility of engineering infectious viral cDNA for construction of hypovirulent fungal strains with specific phenotypic traits.

This article has been cited by other articles:

• Deng, F., Nuss, D. L. (2008). Hypovirus Papain-Like Protease p48 Is Required for Initiation but Not for Maintenance of Virus RNA Propagation in the Chestnut Blight Fungus Cryphonectria parasitica. J. Virol. 82: 6369-6378 [Abstract] [Full Text]  
• Lin, H., Lan, X., Liao, H., Parsley, T. B., Nuss, D. L., Chen, B. (2007). Genome Sequence, Full-Length Infectious cDNA Clone, and Mapping of Viral Double-Stranded RNA Accumulation Determinant of Hypovirus CHV1-EP721. J. Virol. 81: 1813-1820 [Abstract] [Full Text]  
• Deng, F., Allen, T. D., Nuss, D. L. (2007). Ste12 Transcription Factor Homologue CpST12 Is Down-Regulated by Hypovirus Infection and Required for Virulence and Female Fertility of the Chestnut Blight Fungus Cryphonectria parasitica. Eukaryot Cell 6: 235-244 [Abstract] [Full Text]  
• Sun, L., Nuss, D. L., Suzuki, N. (2006). Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713. J. Gen. Virol. 87: 3703-3714 [Abstract] [Full Text]  
• Jacob-Wilk, D., Turina, M., Van Alfen, N. K. (2006). Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica.. J. Virol. 80: 6588-6596 [Abstract] [Full Text]  
• Segers, G. C., van Wezel, R., Zhang, X., Hong, Y., Nuss, D. L. (2006). Hypovirus Papain-Like Protease p29 Suppresses RNA Silencing in the Natural Fungal Host and in a Heterologous Plant System. Eukaryot Cell 5: 896-904 [Abstract] [Full Text]  
• Xie, J., Wei, D., Jiang, D., Fu, Y., Li, G., Ghabrial, S., Peng, Y. (2006). Characterization of debilitation-associated mycovirus infecting the plant-pathogenic fungus Sclerotinia sclerotiorum. J. Gen. Virol. 87: 241-249 [Abstract] [Full Text]  
• Segers, G. C., Regier, J. C., Nuss, Donald. L. (2004). Evidence for a Role of the Regulator of G-Protein Signaling Protein CPRGS-1 in G{alpha} Subunit CPG-1-Mediated Regulation of Fungal Virulence, Conidiation, and Hydrophobin Synthesis in the Chestnut Blight Fungus Cryphonectria parasitica. Eukaryot Cell 3: 1454-1463 [Abstract] [Full Text]  
• Allen, T. D., Dawe, A. L., Nuss, D. L. (2003). Use of cDNA Microarrays To Monitor Transcriptional Responses of the Chestnut Blight Fungus Cryphonectria parasitica to Infection by Virulence-Attenuating Hypoviruses. Eukaryot Cell 2: 1253-1265 [Abstract] [Full Text]  
• Moleleki, N., van Heerden, S. W., Wingfield, M. J., Wingfield, B. D., Preisig, O. (2003). Transfection of Diaporthe perjuncta with Diaporthe RNA Virus. Appl. Environ. Microbiol. 69: 3952-3956 [Abstract] [Full Text]  
• Suzuki, N., Nuss, D. L. (2002). Contribution of Protein p40 to Hypovirus-Mediated Modulation of Fungal Host Phenotype and Viral RNA Accumulation. J. Virol. 76: 7747-7759 [Abstract] [Full Text]  
• Parsley, T. B., Chen, B., Geletka, L. M., Nuss, D. L. (2002). Differential Modulation of Cellular Signaling Pathways by Mild and Severe Hypovirus Strains. Eukaryot Cell 1: 401-413 [Abstract] [Full Text]  
• Peng, C.-W., Peremyslov, V. V., Mushegian, A. R., Dawson, W. O., Dolja, V. V. (2001). Functional Specialization and Evolution of Leader Proteinases in the Family Closteroviridae. J. Virol. 75: 12153-12160 [Abstract] [Full Text]  
• Peng, C.-W., Dolja, V. V. (2000). Leader Proteinase of the Beet Yellows Closterovirus: Mutation Analysis of the Function in Genome Amplification. J. Virol. 74: 9766-9770 [Abstract] [Full Text]  
• Suzuki, N., Geletka, L. M., Nuss, D. L. (2000). Essential and Dispensable Virus-Encoded Replication Elements Revealed by Efforts To Develop Hypoviruses as Gene Expression Vectors. J. Virol. 74: 7568-7577 [Abstract] [Full Text]  
• Kasahara, S., Wang, P., Nuss, D. L. (2000). Identification of bdm-1, a gene involved in G protein beta -subunit function and alpha -subunit accumulation. Proc. Natl. Acad. Sci. USA 97: 412-417 [Abstract] [Full Text]  
• Suzuki, N., Chen, B., Nuss, D. L. (1999). Mapping of a Hypovirus p29 Protease Symptom Determinant Domain with Sequence Similarity to Potyvirus HC-Pro Protease. J. Virol. 73: 9478-9484 [Abstract] [Full Text]  
• Chen, B., Nuss, D. L. (1999). Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica. J. Virol. 73: 985-992 [Abstract] [Full Text]  
• Peremyslov, V. V., Hagiwara, Y., Dolja, V. V. (1998). Genes Required for Replication of the 15.5-Kilobase RNA Genome of a Plant Closterovirus. J. Virol. 72: 5870-5876 [Abstract] [Full Text]  
• Chen, B, Choi, G., Nuss, D. (1994). Attenuation of fungal virulence by synthetic infectious hypovirus transcripts. Science 264: 1762-1764 [Abstract]