This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Deminie, C A Articles by Emerman, M Search for Related Content PubMed PubMed Citation Articles by Deminie, C A Articles by Emerman, M

Incorporation of human immunodeficiency virus type 1 Gag proteins into murine leukemia virus virions.

Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.

ABSTRACT

The retroviral Gag polyprotein is necessary and sufficient for assembly and budding of viral particles. However, the exact inter- and intramolecular interactions of the Gag polyproteins during this process are not known. To locate functional domains within Gag, we generated chimeric proviruses between human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MuLV). In these chimeric proviruses, the matrix or capsid proteins of MuLV were precisely replaced with the matrix or capsid proteins of HIV-1. Although the chimeric proviruses were unable to efficiently assemble into mature viral particles by themselves, coexpression of wild-type MuLV Gag rescued the HIV proteins into virions. The specificity of the rescue of HIV proteins into MuLV virions shows that specific interactions involving homologous matrix or capsid regions of Gag are necessary for retroviral particle formation.

This article has been cited by other articles:

• Shun, M.-C., Daigle, J. E., Vandegraaff, N., Engelman, A. (2007). Wild-Type Levels of Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Cellular Emerin Protein. J. Virol. 81: 166-172 [Abstract] [Full Text]  
• Piccirillo, R., Palmisano, I., Innamorati, G., Bagnato, P., Altimare, D., Schiaffino, M. V. (2006). An unconventional dileucine-based motif and a novel cytosolic motif are required for the lysosomal and melanosomal targeting of OA1. J. Cell Sci. 119: 2003-2014 [Abstract] [Full Text]  
• Ako-Adjei, D., Johnson, M. C., Vogt, V. M. (2005). The Retroviral Capsid Domain Dictates Virion Size, Morphology, and Coassembly of Gag into Virus-Like Particles. J. Virol. 79: 13463-13472 [Abstract] [Full Text]  
• Sakalian, M., Dittmer, S. S., Gandy, A. D., Rapp, N. D., Zabransky, A., Hunter, E. (2002). The Mason-Pfizer Monkey Virus Internal Scaffold Domain Enables In Vitro Assembly of Human Immunodeficiency Virus Type 1 Gag. J. Virol. 76: 10811-10820 [Abstract] [Full Text]  
• Reed, M., Mariani, R., Sheppard, L., Pekrun, K., Landau, N. R., Soong, N.-W. (2002). Chimeric Human Immunodeficiency Virus Type 1 Containing Murine Leukemia Virus Matrix Assembles in Murine Cells. J. Virol. 76: 436-443 [Abstract] [Full Text]  
• Chouteau, P., Le Seyec, J., Cannie, I., Nassal, M., Guguen-Guillouzo, C., Gripon, P. (2001). A Short N-Proximal Region in the Large Envelope Protein Harbors a Determinant That Contributes to the Species Specificity of Human Hepatitis B Virus. J. Virol. 75: 11565-11572 [Abstract] [Full Text]  
• Buchschacher, G. L. Jr., Yu, L., Murai, F., Friedmann, T., Miyanohara, A. (1999). Association of Murine Leukemia Virus Pol with Virions, Independent of Gag-Pol Expression. J. Virol. 73: 9632-9637 [Abstract] [Full Text]  
• Johnston, J. C., Gasmi, M., Lim, L. E., Elder, J. H., Yee, J.-K., Jolly, D. J., Campbell, K. P., Davidson, B. L., Sauter, S. L. (1999). Minimum Requirements for Efficient Transduction of Dividing and Nondividing Cells by Feline Immunodeficiency Virus Vectors. J. Virol. 73: 4991-5000 [Abstract] [Full Text]  
• Garoff, H., Hewson, R., Opstelten, D.-J. E. (1998). Virus Maturation by Budding. Microbiol. Mol. Biol. Rev. 62: 1171-1190 [Abstract] [Full Text]  
• Weldon, R. A. Jr., Parker, W. B., Sakalian, M., Hunter, E. (1998). Type D Retrovirus Capsid Assembly and Release Are Active Events Requiring ATP. J. Virol. 72: 3098-3106 [Abstract] [Full Text]  
• Diamond, S. E., Gutierrez-Hartmann, A. (1996). A 26-Amino Acid Insertion Domain Defines a Functional Transcription Switch Motif in Pit-1beta. J. Biol. Chem. 271: 28925-28932 [Abstract] [Full Text]