This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bridge, E
Right arrow Articles by Pettersson, U
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bridge, E
Right arrow Articles by Pettersson, U

 Previous Article  |  Next Article 

J Virol. 1993 October; 67(10): 5792-5802

Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells.

E Bridge, M Carmo-Fonseca, A Lamond and U Pettersson

Department of Medical Genetics, Uppsala University, Sweden.

ABSTRACT

We have studied the effect of adenovirus infection on the nuclear organization of splicing small nuclear ribonucleoproteins (snRNPs) in HeLa cells. In uninfected HeLa cells, snRNPs are widespread throughout the nucleoplasm but also are concentrated in specific nuclear structures, including coiled bodies, interchromatin granules, and perichromatin fibrils. We have used immunofluorescence microscopy to study the localization of splicing snRNPs relative to centers of viral DNA synthesis and accumulation identified with antiserum against the viral 72,000-molecular-weight single-stranded DNA-binding protein (72K protein). Splicing snRNPs were independently detected with both monoclonal and polyclonal antibodies specific for common snRNP antigens, snRNP-specific proteins, and the snRNA-specific 2,2,7-trimethylguanosine 5' cap structure. We have examined infected cells 2 to 24 h after infection, and, in the majority of these cells, we observed no colocalization of the snRNP and 72K-protein staining patterns. In the late phase, snRNPs were found to markedly concentrate in discrete clusters that were distinct from the centers of viral DNA synthesis and accumulation identified with anti-72K protein. We have treated cells with hydroxyurea at various times after infection to inhibit aspects of the virus infectious program. We have found that the accumulation of snRNP clusters is correlated with late gene expression rather than with DNA synthesis or early gene expression. Finally, we show that the late-phase snRNP clusters colocalize with a monoclonal antibody that primarily stains interchromatin granules. These results suggest that the centers of snRNP concentration in late-phase infected cells are likely to correspond to interchromatin granule clusters.

J Virol. 1993 October; 67(10): 5792-5802




This article has been cited by other articles:

  • Marshall, L. J., Moore, A. C., Ohki, M., Kitabayashi, I., Patterson, D., Ornelles, D. A. (2008). RUNX1 Permits E4orf6-Directed Nuclear Localization of the Adenovirus E1B-55K Protein and Associates with Centers of Viral DNA and RNA Synthesis. J. Virol. 82: 6395-6408 [Abstract] [Full Text]  
  • Lindberg, A., Gama-Carvalho, M., Carmo-Fonseca, M., Kreivi, J.-P. (2004). A single RNA recognition motif in splicing factor ASF/SF2 directs it to nuclear sites of adenovirus transcription. J. Gen. Virol. 85: 603-608 [Abstract] [Full Text]  
  • Lee, T. W. R., Lawrence, F. J., Dauksaite, V., Akusjarvi, G., Blair, G. E., Matthews, D. A. (2004). Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins. J. Gen. Virol. 85: 185-196 [Abstract] [Full Text]  
  • Rabino, C., Aspegren, A., Corbin-Lickfett, K., Bridge, E. (2000). Adenovirus Late Gene Expression Does Not Require a Rev-Like Nuclear RNA Export Pathway. J. Virol. 74: 6684-6688 [Abstract] [Full Text]  
  • Misteli, T (2000). Cell biology of transcription and pre-mRNA splicing: nuclear architecture meets nuclear function. J. Cell Sci. 113: 1841-1849 [Abstract]  
  • Angeletti, P. C., Engler, J. A. (1998). Adenovirus Preterminal Protein Binds to the CAD Enzyme at Active Sites of Viral DNA Replication on the Nuclear Matrix. J. Virol. 72: 2896-2904 [Abstract] [Full Text]  
  • Sanchez, V., Angeletti, P. C., Engler, J. A., Britt, W. J. (1998). Localization of Human Cytomegalovirus Structural Proteins to the Nuclear Matrix of Infected Human Fibroblasts. J. Virol. 72: 3321-3329 [Abstract] [Full Text]  
  • Huang, W., Flint, S. J. (1998). The Tripartite Leader Sequence of Subgroup C Adenovirus Major Late mRNAs Can Increase the Efficiency of mRNA Export. J. Virol. 72: 225-235 [Abstract] [Full Text]  
  • Gama-Carvalho, M., Krauss, R. D., Chiang, L., Valcarcel, J., Green, M. R., Carmo-Fonseca, M. (1997). Targeting of U2AF65 to Sites of Active Splicing in the Nucleus. JCB 137: 975-987 [Abstract] [Full Text]  
  • Puvion-Dutilleul, F, Bachellerie, J., Visa, N, Puvion, E (1994). Rearrangements of intranuclear structures involved in RNA processing in response to adenovirus infection. J. Cell Sci. 107: 1457-1468 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»