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Release, uptake, and effects of extracellular human immunodeficiency virus type 1 Tat protein on cell growth and viral transactivation.

Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20892.

ABSTRACT

During acute human immunodeficiency virus type 1 (HIV-1) infection or after transfection of the tat gene, Tat protein is released into the cell culture supernatant. In this extracellular form, Tat stimulates both HIV-1 gene expression and the growth of cells derived from Kaposi's sarcoma (KS) lesions of HIV-1-infected individuals (AIDS-KS cells). Tat protein and its biological activities appear in the cell supernatants at the peak of Tat expression, when the rate of cell death is low (infection) or cell death is undetectable (transfection) and increased levels of cytoplasmic Tat are present. Tat-containing supernatants stimulate maximal AIDS-KS cell growth but only low to moderate levels of HIV-1 gene expression. This is due to the different concentrations of exogenous Tat required for the two effects. The cell growth-promoting effects of Tat peak at between 0.1 and 1 ng of purified recombinant protein per ml in the cell growth medium and do not increase with concentration. In contrast, both the detection of nuclear-localized Tat taken up by cells and the induction of HIV-1 gene expression or replication require higher Tat concentrations (> or = 100 ng/ml), and all increase linearly with increasing amounts of the exogenous protein. These data suggest that Tat can be released by a mechanism(s) other than cell death and that the cell growth-promoting activity and the virus-transactivating effect of extracellular Tat are mediated by different pathways.

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• Agwale, S. M., Shata, M. T., Reitz, M. S. Jr., Kalyanaraman, V. S., Gallo, R. C., Popovic, M., Hone, D. M. (2002). A Tat subunit vaccine confers protective immunity against the immune-modulating activity of the human immunodeficiency virus type-1 Tat protein in mice. Proc. Natl. Acad. Sci. USA 99: 10037-10041 [Abstract] [Full Text]  
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• Arese, M., Ferrandi, C., Primo, L., Camussi, G., Bussolino, F. (2001). HIV-1 Tat Protein Stimulates In Vivo Vascular Permeability and Lymphomononuclear Cell Recruitment. J. Immunol. 166: 1380-1388 [Abstract] [Full Text]  
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• Kutsch, O., Oh, J.-W., Nath, A., Benveniste, E. N. (2000). Induction of the Chemokines Interleukin-8 and IP-10 by Human Immunodeficiency Virus Type 1 Tat in Astrocytes. J. Virol. 74: 9214-9221 [Abstract] [Full Text]  
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• Altavilla, G., Caputo, A., Lanfredi, M., Piola, C., Barbanti-Brodano, G., Corallini, A. (2000). Enhancement of Chemical Hepatocarcinogenesis by the HIV-1 tat Gene. Am. J. Pathol. 157: 1081-1089 [Abstract] [Full Text]  
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• Srinivasakumar, N., Schuening, F. (2000). Novel Tat-Encoding Bicistronic Human Immunodeficiency Virus Type 1-Based Gene Transfer Vectors for High-Level Transgene Expression. J. Virol. 74: 6659-6668 [Abstract] [Full Text]  
• Mayne, M., Holden, C. P., Nath, A., Geiger, J. D. (2000). Release of Calcium from Inositol 1,4,5-Trisphosphate Receptor-Regulated Stores by HIV-1 Tat Regulates TNF-{alpha} Production in Human Macrophages. J. Immunol. 164: 6538-6542 [Abstract] [Full Text]  
• Mercader, M., Taddeo, B., Panella, J. R., Chandran, B., Nickoloff, B. J., Foreman, K. E. (2000). Induction of HHV-8 Lytic Cycle Replication by Inflammatory Cytokines Produced by HIV-1-Infected T Cells. Am. J. Pathol. 156: 1961-1971 [Abstract] [Full Text]  
• Manna, S. K., Aggarwal, B. B. (2000). Differential Requirement for p56lck in HIV-tat Versus TNF-Induced Cellular Responses: Effects on NF-{kappa}B, Activator Protein-1, c-Jun N-Terminal Kinase, and Apoptosis. J. Immunol. 164: 5156-5166 [Abstract] [Full Text]  
• Prakash, O., Tang, Z.-Y., He, Y.-e, Ali, M. S., Coleman, R., Gill, J., Farr, G., Samaniego, F. (2000). Human Kaposi's Sarcoma Cell-Mediated Tumorigenesis in Human Immunodeficiency Type 1 Tat-Expressing Transgenic Mice. JNCI J Natl Cancer Inst 92: 721-728 [Abstract] [Full Text]  
• Stone, D. M., Norton, L. K., Davis, W. C. (2000). Spontaneously proliferating lymphocytes from bovine leukaemia virus-infected, lymphocytotic cattle are not the virus-expressing lymphocytes, as these cells are delayed in G0/G1 of the cell cycle and are spared from apoptosis. J. Gen. Virol. 81: 971-981 [Abstract] [Full Text]  
• McManus, C. M., Weidenheim, K., Woodman, S. E., Nunez, J., Hesselgesser, J., Nath, A., Berman, J. W. (2000). Chemokine and Chemokine-Receptor Expression in Human Glial Elements : Induction by the HIV Protein, Tat, and Chemokine Autoregulation. Am. J. Pathol. 156: 1441-1453 [Abstract] [Full Text]  
• Chen, H., He, J., Fong, S., Wilcox, G., Wood, C. (2000). Jembrana Disease Virus Tat Can Regulate Human Immunodeficiency Virus (HIV) Long Terminal Repeat-Directed Gene Expression and Can Substitute for HIV Tat in Viral Replication. J. Virol. 74: 2703-2713 [Abstract] [Full Text]  
• Lim, S. P., Garzino-Demo, A. (2000). The Human Immunodeficiency Virus Type 1 Tat Protein Up-Regulates the Promoter Activity of the Beta-Chemokine Monocyte Chemoattractant Protein 1 in the Human Astrocytoma Cell Line U-87 MG: Role of SP-1, AP-1, and NF-kappa B Consensus Sites. J. Virol. 74: 1632-1640 [Abstract] [Full Text]  
• Annunziato, F., Galli, G., Nappi, F., Cosmi, L., Manetti, R., Maggi, E., Ensoli, B., Romagnani, S. (2000). Limited expression of R5-tropic HIV-1 in CCR5-positive type 1-polarized T cells explained by their ability to produce RANTES, MIP-1alpha , and MIP-1beta. Blood 95: 1167-1174 [Abstract] [Full Text]  
• Mitola, S., Soldi, R., Zanon, I., Barra, L., Gutierrez, M. I., Berkhout, B., Giacca, M., Bussolino, F. (2000). Identification of Specific Molecular Structures of Human Immunodeficiency Virus Type 1 Tat Relevant for Its Biological Effects on Vascular Endothelial Cells. J. Virol. 74: 344-353 [Abstract] [Full Text]  
• Rusnati, M., Tulipano, G., Spillmann, D., Tanghetti, E., Oreste, P., Zoppetti, G., Giacca, M., Presta, M. (1999). Multiple Interactions of HIV-I Tat Protein with Size-defined Heparin Oligosaccharides. J. Biol. Chem. 274: 28198-28205 [Abstract] [Full Text]  
• Reitz, M. S. Jr., Nerurkar, L. S., Gallo, R. C. (1999). Perspective on Kaposi's Sarcoma: Facts, Concepts, and Conjectures. JNCI J Natl Cancer Inst 91: 1453-1458 [Full Text]  
• Weiss, J. M., Nath, A., Major, E. O., Berman, J. W. (1999). HIV-1 Tat Induces Monocyte Chemoattractant Protein-1-Mediated Monocyte Transmigration Across a Model of the Human Blood-Brain Barrier and Up-Regulates CCR5 Expression on Human Monocytes. J. Immunol. 163: 2953-2959 [Abstract] [Full Text]  
• Chirivi, R. G.S., Taraboletti, G., Bani, M. R., Barra, L., Piccinini, G., Giacca, M., Bussolino, F., Giavazzi, R. (1999). Human Immunodeficiency Virus-1 (HIV-1)-Tat Protein Promotes Migration of Acquired Immunodeficiency Syndrome-Related Lymphoma Cells and Enhances Their Adhesion to Endothelial Cells. Blood 94: 1747-1754 [Abstract] [Full Text]  
• Barillari, G., Sgadari, C., Palladino, C., Gendelman, R., Caputo, A., Morris, C. B., Nair, B. C., Markham, P., Nel, A., Sturzl, M., Ensoli, B. (1999). Inflammatory Cytokines Synergize with the HIV-1 Tat Protein to Promote Angiogenesis and Kaposi's Sarcoma Via Induction of Basic Fibroblast Growth Factor and the {alpha}v{beta}3 Integrin. J. Immunol. 163: 1929-1935 [Abstract] [Full Text]  
• Barillari, G., Sgadari, C., Fiorelli, V., Samaniego, F., Colombini, S., Manzari, V., Modesti, A., Nair, B. C., Cafaro, A., Sturzl, M., Ensoli, B. (1999). The Tat Protein of Human Immunodeficiency Virus Type-1 Promotes Vascular Cell Growth and Locomotion by Engaging the alpha 5beta 1 and alpha vbeta 3 Integrins and by Mobilizing Sequestered Basic Fibroblast Growth Factor. Blood 94: 663-672 [Abstract] [Full Text]  
• Boykins, R. A., Mahieux, R., Shankavaram, U. T., Gho, Y. S., Lee, S. F., Hewlett, I. K., Wahl, L. M., Kleinman, H. K., Brady, J. N., Yamada, K. M., Dhawan, S. (1999). Cutting Edge: A Short Polypeptide Domain of HIV-1-Tat Protein Mediates Pathogenesis. J. Immunol. 163: 15-20 [Abstract] [Full Text]  
• Ascherl, G., Hohenadl, C., Schatz, O., Shumay, E., Bogner, J., Eckhart, L., Tschachler, E., Monini, P., Ensoli, B., Sturzl, M. (1999). Infection With Human Immunodeficiency Virus-1 Increases Expression of Vascular Endothelial Cell Growth Factor in T Cells: Implications for Acquired Immunodeficiency Syndrome-Associated Vasculopathy. Blood 93: 4232-4241 [Abstract] [Full Text]  
• Nath, A., Conant, K., Chen, P., Scott, C., Major, E. O. (1999). Transient Exposure to HIV-1 Tat Protein Results in Cytokine Production in Macrophages and Astrocytes. A HIT AND RUN PHENOMENON. J. Biol. Chem. 274: 17098-17102 [Abstract] [Full Text]  
• Caselli, E., Betti, M., Grossi, M. P., Balboni, P. G., Rossi, C., Boarini, C., Cafaro, A., Barbanti-Brodano, G., Ensoli, B., Caputo, A. (1999). DNA Immunization with HIV-1 tat Mutated in the trans Activation Domain Induces Humoral and Cellular Immune Responses Against Wild-Type Tat. J. Immunol. 162: 5631-5638 [Abstract] [Full Text]  
• Altavilla, G., Trabanelli, C., Merlin, M., Caputo, A., Lanfredi, M., Barbanti-Brodano, G., Corallini, A. (1999). Morphological, Histochemical, Immunohistochemical, and Ultrastructural Characterization of Tumors and Dysplastic and Non-Neoplastic Lesions Arising in BK Virus/tat Transgenic Mice. Am. J. Pathol. 154: 1231-1244 [Abstract] [Full Text]  
• Fiorelli, V., Barillari, G., Toschi, E., Sgadari, C., Monini, P., Sturzl, M., Ensoli, B. (1999). IFN-{gamma} Induces Endothelial Cells to Proliferate and to Invade the Extracellular Matrix in Response to the HIV-1 Tat Protein: Implications for AIDS-Kaposi's Sarcoma Pathogenesis. J. Immunol. 162: 1165-1170 [Abstract] [Full Text]  
• Kino, T., Gragerov, A., Kopp, J. B., Stauber, R. H., Pavlakis, G. N., Chrousos, G. P. (1999). The HIV-1 Virion-associated Protein Vpr Is a Coactivator of the Human Glucocorticoid Receptor. J. Exp. Med. 189: 51-62 [Abstract] [Full Text]  
• Huang, L., Bosch, I., Hofmann, W., Sodroski, J., Pardee, A. B. (1998). Tat Protein Induces Human Immunodeficiency Virus Type 1 (HIV-1) Coreceptors and Promotes Infection with both Macrophage-Tropic and T-Lymphotropic HIV-1 Strains. J. Virol. 72: 8952-8960 [Abstract] [Full Text]  
• Kumar, A., Manna, S. K., Dhawan, S., Aggarwal, B. B. (1998). HIV-Tat Protein Activates c-Jun N-Terminal Kinase and Activator Protein-1. J. Immunol. 161: 776-781 [Abstract] [Full Text]  
• Ganju, R. K., Munshi, N., Nair, B. C., Liu, Z.-Y., Gill, P., Groopman, J. E. (1998). Human Immunodeficiency Virus Tat Modulates the Flk-1/KDR Receptor, Mitogen-Activated Protein Kinases, and Components of Focal Adhesion in Kaposi's Sarcoma Cells. J. Virol. 72: 6131-6137 [Abstract] [Full Text]  
• Albini, A., Benelli, R., Giunciuglio, D., Cai, T., Mariani, G., Ferrini, S., Noonan, D. M. (1998). Identification of a Novel Domain of HIV Tat Involved in Monocyte Chemotaxis. J. Biol. Chem. 273: 15895-15900 [Abstract] [Full Text]  
• Rusnati, M., Tulipano, G., Urbinati, C., Tanghetti, E., Giuliani, R., Giacca, M., Ciomei, M., Corallini, A., Presta, M. (1998). The Basic Domain in HIV-1 Tat Protein as a Target for Polysulfonated Heparin-mimicking Extracellular Tat Antagonists. J. Biol. Chem. 273: 16027-16037 [Abstract] [Full Text]  
• Gibellini, D., Bassini, A., Pierpaoli, S., Bertolaso, L., Milani, D., Capitani, S., La Placa, M., Zauli, G. (1998). Extracellular HIV-1 Tat Protein Induces the Rapid Ser133 Phosphorylation and Activation of CREB Transcription Factor in Both Jurkat Lymphoblastoid T Cells and Primary Peripheral Blood Mononuclear Cells. J. Immunol. 160: 3891-3898 [Abstract] [Full Text]  
• Gallo, R. C. (1998). Some Aspects of the Pathogenesis of HIV-1-Associated Kaposi's Sarcoma. J Natl Cancer Inst Monogr 1998: 55-57 [Abstract] [Full Text]  
• Conant, K., Garzino-Demo, A., Nath, A., McArthur, J. C., Halliday, W., Power, C., Gallo, R. C., Major, E. O. (1998). Induction of monocyte chemoattractant protein-1 in HIV-1 Tat-stimulated astrocytes and elevation in AIDS dementia. Proc. Natl. Acad. Sci. USA 95: 3117-3121 [Abstract] [Full Text]