This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oppenheim, A Articles by Ottolenghi, S Search for Related Content PubMed PubMed Citation Articles by Oppenheim, A Articles by Ottolenghi, S

A cis-acting DNA signal for encapsidation of simian virus 40.

Department of Hematology, Hadassah University Hospital, Jerusalem, Israel.

ABSTRACT

Encapsidation of simian virus 40 is a complex biological process involving DNA-protein and protein-protein interactions in the formation of a unique three-dimensional structure around the viral minichromosome. A pseudoviral system developed in our laboratory, in which the viral early and late gene products are supplied in trans (by helpers), was used to analyze the encapsidation process independent of viral gene expression. With this experimental system we have discovered a requirement for a specific DNA signal for encapsidation, ses (for simian virus 40 encapsidation signal).ses is present within a 200-bp DNA fragment, which includes, in addition to the viral origin of replication (ori), six GGGCGG repeats (GC boxes) and 26 bp of the enhancer element. Deletion of the GC boxes and the enhancer sequences almost abolished encapsidation, while DNA replication was only moderately decreased. The ability to encapsidate was not regained by reinserting a DNA fragment carrying ses in the sesdeleted plasmid 2 kbp away from the ori, suggesting that for encapsidation the two DNA elements have to be close to each other. These findings afford novel strategies for the investigation of viral encapsidation.

This article has been cited by other articles:

• Carbone, M., Ascione, G., Chichiarelli, S., Garcia, M.-I., Eufemi, M., Amati, P. (2004). Chromosome-Protein Interactions in Polyomavirus Virions. J. Virol. 78: 513-519 [Abstract] [Full Text]  
• Erturk, E., Ostapchuk, P., Wells, S. I., Yang, J., Gregg, K., Nepveu, A., Dudley, J. P., Hearing, P. (2003). Binding of CCAAT Displacement Protein CDP to Adenovirus Packaging Sequences. J. Virol. 77: 6255-6264 [Abstract] [Full Text]  
• Gordon-Shaag, A., Ben-Nun-Shaul, O., Roitman, V., Yosef, Y., Oppenheim, A. (2002). Cellular Transcription Factor Sp1 Recruits Simian Virus 40 Capsid Proteins to the Viral Packaging Signal, ses. J. Virol. 76: 5915-5924 [Abstract] [Full Text]  
• Li, P. P., Nakanishi, A., Shum, D., Sun, P. C.-K., Salazar, A. M., Fernandez, C. F., Chan, S.-W., Kasamatsu, H. (2001). Simian Virus 40 Vp1 DNA-Binding Domain Is Functionally Separable from the Overlapping Nuclear Localization Signal and Is Required for Effective Virion Formation and Full Viability. J. Virol. 75: 7321-7329 [Abstract] [Full Text]  
• Day, P. M., Roden, R. B. S., Lowy, D. R., Schiller, J. T. (1998). The Papillomavirus Minor Capsid Protein, L2, Induces Localization of the Major Capsid Protein, L1, and the Viral Transcription/Replication Protein, E2, to PML Oncogenic Domains. J. Virol. 72: 142-150 [Abstract] [Full Text]