This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miller, M L Articles by Brown, D T Search for Related Content PubMed PubMed Citation Articles by Miller, M L Articles by Brown, D T

 Previous Article  |  Next Article 

J Virol. 1992 July; 66(7): 4180-4190

Morphogenesis of Sindbis virus in three subclones of Aedes albopictus (mosquito) cells.

Cell Research Institute, University of Texas, Austin 78713-7640.

ABSTRACT

The morphogenesis of Sindbis virus in three Aedes albopictus subcloned cell lines was examined. Each line was distinguishable with respect to morphology, cytopathic response to infection, and progeny yield. C7-10 cells, which produced the highest titers of virus and exhibited the most severe cytopathic response, were characterized ultrastructurally by the presence of budding particles at the cell surface and at the membranes of internal vesicles. C6/36 cells, which displayed a moderate cytotoxic response, manifested similar features in response to Sindbis virus infection. Both cell types also produced a structure composed of an electron-dense matrix in which nucleocapsids were embedded. Internally matured virions were released by exocytosis from these cells. In addition to a lack of cytopathic effect, u4.4 cells also failed to exhibit obvious morphogenetic changes upon infection. Virus particles were occasionally seen within vesicles, but budding at the cell surface was not detected. The mechanism of release of internally matured virions was not apparent. These studies provide further evidence that these three subcloned mosquito cell lines represent different tissues in the larval or adult insect.

This article has been cited by other articles:

• West, J., Hernandez, R., Ferreira, D., Brown, D. T. (2006). Mutations in the Endodomain of Sindbis Virus Glycoprotein E2 Define Sequences Critical for Virus Assembly. J. Virol. 80: 4458-4468 [Abstract] [Full Text]  
• Hernandez, R., Ferreira, D., Sinodis, C., Litton, K., Brown, D. T. (2005). Single Amino Acid Insertions at the Junction of the Sindbis Virus E2 Transmembrane Domain and Endodomain Disrupt Virus Envelopment and Alter Infectivity. J. Virol. 79: 7682-7697 [Abstract] [Full Text]  
• Hernandez, R., Sinodis, C., Horton, M., Ferreira, D., Yang, C., Brown, D. T. (2003). Deletions in the Transmembrane Domain of a Sindbis Virus Glycoprotein Alter Virus Infectivity, Stability, and Host Range. J. Virol. 77: 12710-12719 [Abstract] [Full Text]  
• Boehme, K. W., Popov, V. L., Heidner, H. W. (2000). The Host Range Phenotype Displayed by a Sindbis Virus Glycoprotein Variant Results from Virion Aggregation and Retention on the Surface of Mosquito Cells. J. Virol. 74: 11398-11406 [Abstract] [Full Text]  
• Hernandez, R., Lee, H., Nelson, C., Brown, D. T. (2000). A Single Deletion in the Membrane-Proximal Region of the Sindbis Virus Glycoprotein E2 Endodomain Blocks Virus Assembly. J. Virol. 74: 4220-4228 [Abstract] [Full Text]  
• Boehme, K. W., Williams, J. C., Johnston, R. E., Heidner, H. W. (2000). Linkage of an alphavirus host-range restriction to the carbohydrate-processing phenotypes of the host cell. J. Gen. Virol. 81: 161-170 [Abstract] [Full Text]