This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Halbert, C L Articles by Galloway, D A Search for Related Content PubMed PubMed Citation Articles by Halbert, C L Articles by Galloway, D A

 Previous Article  |  Next Article 

J Virol. 1992 April; 66(4): 2125-2134

The E6 and E7 genes of human papillomavirus type 6 have weak immortalizing activity in human epithelial cells.

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.

ABSTRACT

Previous studies have shown that the E7 gene of human papillomavirus (HPV) type 16 or 18 alone was sufficient for immortalization of human foreskin epithelial cells (HFE) and that the efficiency was increased in cooperation with the respective E6 gene, whereas the HPV6 E6 or E7 gene was not active in HFE. To detect weak immortalizing activities of the HPV6 genes, cells were infected with recombinant retroviruses containing HPV genes, alone and in homologous and heterologous combinations. The HPV6 genes, alone or together (HPV6 E6 plus HPV6 E7), were not able to immortalize cells. However the HPV6 E6 gene, in concert with HPV16 E7, increased the frequency of immortalization threefold over that obtained with HPV16 E7 alone. Interestingly, 6 of 20 clones containing the HPV16 E6 gene and the HPV6 E7 gene were immortalized, whereas neither gene alone was sufficient. Thus, the HPV6 E6 and E7 genes have weak immortalizing activities which can be detected in cooperation with the more active transforming genes of HPV16. Acute expression of the HPV6 and HPV16 E6 and E7 genes revealed that only HPV16 E7 was able to stimulate the proliferation of cells in organotypic culture, resulting in increased expression of the proliferative cell nuclear antigen and the formation of a disorganized epithelial layer. Additionally, combinations of genes that immortalized HFE cells (HPV16 E6 plus HPV16 E7, HPV16 E6 plus HPV6 E7, and HPV6 E6 plus HPV16 E7) also stimulated proliferation.

This article has been cited by other articles:

• Insinga, R. P., Liaw, K.-L., Johnson, L. G., Madeleine, M. M. (2008). A Systematic Review of the Prevalence and Attribution of Human Papillomavirus Types among Cervical, Vaginal, and Vulvar Precancers and Cancers in the United States. Cancer Epidemiol. Biomarkers Prev. 17: 1611-1622 [Abstract] [Full Text]  
• Shamanin, V. A., Sekaric, P., Androphy, E. J. (2008). hAda3 Degradation by Papillomavirus Type 16 E6 Correlates with Abrogation of the p14ARF-p53 Pathway and Efficient Immortalization of Human Mammary Epithelial Cells. J. Virol. 82: 3912-3920 [Abstract] [Full Text]  
• Das, M., Jiang, F., Sluss, H. K., Zhang, C., Shokat, K. M., Flavell, R. A., Davis, R. J. (2007). Suppression of p53-dependent senescence by the JNK signal transduction pathway. Proc. Natl. Acad. Sci. USA 104: 15759-15764 [Abstract] [Full Text]  
• Wang, K., Cheng, C., Li, L., Liu, H., Huang, Q., Xia, C.-h., Yao, K., Sun, P., Horwitz, J., Gong, X. (2007). {gamma}D-Crystallin Associated Protein Aggregation and Lens Fiber Cell Denucleation. IOVS 48: 3719-3728 [Abstract] [Full Text]  
• Liu, Y., Heilman, S. A., Illanes, D., Sluder, G., Chen, J. J. (2007). p53-Independent Abrogation of a Postmitotic Checkpoint Contributes to Human Papillomavirus E6-Induced Polyploidy. Cancer Res. 67: 2603-2610 [Abstract] [Full Text]  
• Banerjee, N. S., Genovese, N. J., Noya, F., Chien, W.-M., Broker, T. R., Chow, L. T. (2006). Conditionally activated e7 proteins of high-risk and low-risk human papillomaviruses induce s phase in postmitotic, differentiated human keratinocytes.. J. Virol. 80: 6517-6524 [Abstract] [Full Text]  
• Iuchi, S., Dabelsteen, S., Easley, K., Rheinwald, J. G., Green, H. (2006). Immortalized keratinocyte lines derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 103: 1792-1797 [Abstract] [Full Text]  
• Zhang, B., Chen, W., Roman, A. (2006). The E7 proteins of low- and high-risk human papillomaviruses share the ability to target the pRB family member p130 for degradation. Proc. Natl. Acad. Sci. USA 103: 437-442 [Abstract] [Full Text]  
• Peterson, M. L., Ault, K., Kremer, M. J., Klingelhutz, A. J., Davis, C. C., Squier, C. A., Schlievert, P. M. (2005). The Innate Immune System Is Activated by Stimulation of Vaginal Epithelial Cells with Staphylococcus aureus and Toxic Shock Syndrome Toxin 1. Infect. Immun. 73: 2164-2174 [Abstract] [Full Text]  
• Lee, C., Laimins, L. A. (2004). Role of the PDZ Domain-Binding Motif of the Oncoprotein E6 in the Pathogenesis of Human Papillomavirus Type 31. J. Virol. 78: 12366-12377 [Abstract] [Full Text]  
• Verch, T., Pan, Z.-K., Paterson, Y. (2004). Listeria monocytogenes-Based Antibiotic Resistance Gene-Free Antigen Delivery System Applicable to Other Bacterial Vectors and DNA Vaccines. Infect. Immun. 72: 6418-6425 [Abstract] [Full Text]  
• Zou, Y., Sfeir, A., Gryaznov, S. M., Shay, J. W., Wright, W. E. (2004). Does a Sentinel or a Subset of Short Telomeres Determine Replicative Senescence?. Mol. Biol. Cell 15: 3709-3718 [Abstract] [Full Text]  
• Peng, X., Hussain, S. F., Paterson, Y. (2004). The Ability of Two Listeria monocytogenes Vaccines Targeting Human Papillomavirus-16 E7 to Induce an Antitumor Response Correlates with Myeloid Dendritic Cell Function. J. Immunol. 172: 6030-6038 [Abstract] [Full Text]  
• Longworth, M. S., Laimins, L. A. (2004). The Binding of Histone Deacetylases and the Integrity of Zinc Finger-Like Motifs of the E7 Protein Are Essential for the Life Cycle of Human Papillomavirus Type 31. J. Virol. 78: 3533-3541 [Abstract] [Full Text]  
• Patel, D., Incassati, A., Wang, N., McCance, D. J. (2004). Human Papillomavirus Type 16 E6 and E7 Cause Polyploidy in Human Keratinocytes and Up-Regulation of G2-M-Phase Proteins. Cancer Res. 64: 1299-1306 [Abstract] [Full Text]  
• Zabner, J., Karp, P., Seiler, M., Phillips, S. L., Mitchell, C. J., Saavedra, M., Welsh, M., Klingelhutz, A. J. (2003). Development of cystic fibrosis and noncystic fibrosis airway cell lines. Am. J. Physiol. Lung Cell. Mol. Physiol. 284: L844-L854 [Abstract] [Full Text]  
• Fehrmann, F., Klumpp, D. J., Laimins, L. A. (2003). Human Papillomavirus Type 31 E5 Protein Supports Cell Cycle Progression and Activates Late Viral Functions upon Epithelial Differentiation. J. Virol. 77: 2819-2831 [Abstract] [Full Text]  
• Huen, A. C., Park, J. K., Godsel, L. M., Chen, X., Bannon, L. J., Amargo, E. V., Hudson, T. Y., Mongiu, A. K., Leigh, I. M., Kelsell, D. P., Gumbiner, B. M., Green, K. J. (2002). Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength. J. Cell Biol. 159: 1005-1017 [Abstract] [Full Text]  
• Duensing, S., Munger, K. (2002). The Human Papillomavirus Type 16 E6 and E7 Oncoproteins Independently Induce Numerical and Structural Chromosome Instability. Cancer Res. 62: 7075-7082 [Abstract] [Full Text]  
• Gudjonsson, T., Villadsen, R., Nielsen, H. L., Ronnov-Jessen, L., Bissell, M. J., Petersen, O. W. (2002). Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties. Genes Dev. 16: 693-706 [Abstract] [Full Text]  
• Gunn, G. R., Zubair, A., Peters, C., Pan, Z.-K., Wu, T.-C., Paterson, Y. (2001). Two Listeria monocytogenes Vaccine Vectors That Express Different Molecular Forms of Human Papilloma Virus-16 (HPV-16) E7 Induce Qualitatively Different T Cell Immunity That Correlates with Their Ability to Induce Regression of Established Tumors Immortalized by HPV-16. J. Immunol. 167: 6471-6479 [Abstract] [Full Text]  
• Evans, M., Borysiewicz, L. K., Evans, A. S., Rowe, M., Jones, M., Gileadi, U., Cerundolo, V., Man, S. (2001). Antigen Processing Defects in Cervical Carcinomas Limit the Presentation of a CTL Epitope from Human Papillomavirus 16 E6. J. Immunol. 167: 5420-5428 [Abstract] [Full Text]  
• Terhune, S. S., Hubert, W. G., Thomas, J. T., Laimins, L. A. (2001). Early Polyadenylation Signals of Human Papillomavirus Type 31 Negatively Regulate Capsid Gene Expression. J. Virol. 75: 8147-8157 [Abstract] [Full Text]  
• Thomas, J. T., Oh, S. T., Terhune, S. S., Laimins, L. A. (2001). Cellular Changes Induced by Low-Risk Human Papillomavirus Type 11 in Keratinocytes That Stably Maintain Viral Episomes. J. Virol. 75: 7564-7571 [Abstract] [Full Text]  
• Helt, A.-M., Galloway, D. A. (2001). Destabilization of the Retinoblastoma Tumor Suppressor by Human Papillomavirus Type 16 E7 Is Not Sufficient To Overcome Cell Cycle Arrest in Human Keratinocytes. J. Virol. 75: 6737-6747 [Abstract] [Full Text]  
• Bischof, O., Kim, S.-H., Irving, J., Beresten, S., Ellis, N. A., Campisi, J. (2001). Regulation and Localization of the Bloom Syndrome Protein in Response to DNA Damage. J. Cell Biol. 153: 367-380 [Abstract] [Full Text]  
• Degenhardt, Y. Y., Silverstein, S. J. (2001). Gps2, a Protein Partner for Human Papillomavirus E6 Proteins. J. Virol. 75: 151-160 [Abstract] [Full Text]  
• Goodwin, E. C., DiMaio, D. (2000). Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proc. Natl. Acad. Sci. USA 97: 12513-12518 [Abstract] [Full Text]  
• Chien, W.-M., Parker, J. N., Schmidt-Grimminger, D.-C., Broker, T. R., Chow, L. T. (2000). Casein Kinase II Phosphorylation of the Human Papillomavirus-18 E7 Protein Is Critical for Promoting S-Phase Entry. Cell Growth Differ. 11: 425-435 [Abstract] [Full Text]  
• Crish, J. F., Bone, F., Balasubramanian, S., Zaim, T. M., Wagner, T., Yun, J., Rorke, E. A., Eckert, R. L. (2000). Suprabasal expression of the human papillomavirus type 16 oncoproteins in mouse epidermis alters expression of cell cycle regulatory proteins. Carcinogenesis 21: 1031-1037 [Abstract] [Full Text]  
• Dimri, G. P., Itahana, K., Acosta, M., Campisi, J. (2000). Regulation of a Senescence Checkpoint Response by the E2F1 Transcription Factor and p14ARF Tumor Suppressor. Mol. Cell. Biol. 20: 273-285 [Abstract] [Full Text]  
• Johnson, G. A., Burghardt, R. C., Newton, G. R., Bazer, F. W., Spencer, T. E. (1999). Development and Characterization of Immortalized Ovine Endometrial Cell Lines. Biol. Reprod. 61: 1324-1330 [Abstract] [Full Text]  
• Tominaga, K., Morisaki, H., Kaneko, Y., Fujimoto, A., Tanaka, T., Ohtsubo, M., Hirai, M., Okayama, H., Ikeda, K., Nakanishi, M. (1999). Role of Human Cds1 (Chk2) Kinase in DNA Damage Checkpoint and Its Regulation by p53. J. Biol. Chem. 274: 31463-31467 [Abstract] [Full Text]  
• Oram, J. F., Mendez, A. J., Lymp, J., Kavanagh, T. J., Halbert, C. L. (1999). Reduction in apolipoprotein-mediated removal of cellular lipids by immortalization of human fibroblasts and its reversion by cAMP: lack of effect with Tangier disease cells. J. Lipid Res. 40: 1769-1781 [Abstract] [Full Text]  
• Seavey, S. E., Holubar, M., Saucedo, L. J., Perry, M. E. (1999). The E7 Oncoprotein of Human Papillomavirus Type 16 Stabilizes p53 through a Mechanism Independent of p19ARF. J. Virol. 73: 7590-7598 [Abstract] [Full Text]  
• Thomas, J. T., Hubert, W. G., Ruesch, M. N., Laimins, L. A. (1999). Human papillomavirus type 31 oncoproteins E6 and E7 are required for the maintenance of episomes during the viral life cycle in normal human keratinocytes. Proc. Natl. Acad. Sci. USA 96: 8449-8454 [Abstract] [Full Text]  
• Pace, M. C., Chambliss, K. L., German, Z., Yuhanna, I. S., Mendelsohn, M. E., Shaul, P. W. (1999). Establishment of an immortalized fetal intrapulmonary artery endothelial cell line. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L106-L112 [Abstract] [Full Text]  
• Hixon, M. L., Flores, A. I., Wagner, M. W., Gualberto, A. (1998). Ectopic Expression of cdc2/cdc28 Kinase Subunit Homo sapiens 1 Uncouples Cyclin B Metabolism from the Mitotic Spindle Cell Cycle Checkpoint. Mol. Cell. Biol. 18: 6224-6237 [Abstract] [Full Text]  
• Martin, L. G., Demers, G. W., Galloway, D. A. (1998). Disruption of the G1/S Transition in Human Papillomavirus Type 16 E7-Expressing Human Cells Is Associated with Altered Regulation of Cyclin E. J. Virol. 72: 975-985 [Abstract] [Full Text]  
• Thomas, J. T., Laimins, L. A. (1998). Human Papillomavirus Oncoproteins E6 and E7 Independently Abrogate the Mitotic Spindle Checkpoint. J. Virol. 72: 1131-1137 [Abstract] [Full Text]  
• Funk, J. O., Waga, S., Harry, J. B., Espling, E., Stillman, B., Galloway, D. A. (1997). Inhibition of CDK activity and PCNA-dependent DNA replication by p21 is blocked by interaction with the HPV-16 E7 oncoprotein. Genes Dev. 11: 2090-2100 [Abstract] [Full Text]  
• Jones, D. L., Alani, R. M., Munger, K. (1997). The human papillomavirus E7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21Cip1-mediated inhibition of cdk2. Genes Dev. 11: 2101-2111 [Abstract] [Full Text]  
• Linke, S P, Clarkin, K C, Di Leonardo, A, Tsou, A, Wahl, G M (1996). A reversible, p53-dependent G0/G1 cell cycle arrest induced by ribonucleotide depletion in the absence of detectable DNA damage.. Genes Dev. 10: 934-947 [Abstract]  
• Cheng, S, Schmidt-Grimminger, D C, Murant, T, Broker, T R, Chow, L T (1995). Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates cellular DNA replication in suprabasal differentiated keratinocytes.. Genes Dev. 9: 2335-2349 [Abstract]  
• White, A E, Livanos, E M, Tlsty, T D (1994). Differential disruption of genomic integrity and cell cycle regulation in normal human fibroblasts by the HPV oncoproteins.. Genes Dev. 8: 666-677 [Abstract]  
• Chen, M., O'Toole, E. A., Muellenhoff, M., Medina, E., Kasahara, N., Woodley, D. T. (2000). Development and Characterization of A Recombinant Truncated Type VII Collagen "Minigene". IMPLICATION FOR GENE THERAPY OF DYSTROPHIC EPIDERMOLYSIS BULLOSA. J. Biol. Chem. 275: 24429-24435 [Abstract] [Full Text]