This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Battini, J L Articles by Danos, O Search for Related Content PubMed PubMed Citation Articles by Battini, J L Articles by Danos, O

Receptor choice determinants in the envelope glycoproteins of amphotropic, xenotropic, and polytropic murine leukemia viruses.

Laboratoire Rétrovirus et Transfert Génétique, Institut Pasteur, Paris, France.

ABSTRACT

The envelope glycoproteins (SU) of mammalian type C retroviruses possess an amino-terminal domain of about 200 residues, which is involved in binding a cell surface receptor. In this domain, highly conserved amino acid sequences are interrupted by two segments of variable length and sequence, VRA and VRB. We have studied the role of these variable regions in receptor recognition and binding by constructing chimeric molecules in which portions of the amino-terminal domains from amphotropic (4070A), xenotropic (NZB), and polytropic (MCF 247) murine leukemia virus SU proteins were permuted. These chimeras, which exchanged either one or two variable regions, were expressed at the surface of replication-defective viral particles by a pseudotyping assay. Wild-type or recombinant env genes were transfected into a cell line producing Moloney murine leukemia virus particles devoid of envelope glycoproteins in which a retrovirus vector genome carrying an Escherichia coli lacZ gene was packaged. The host range and sensitivity to interference of pseudotyped virions were assayed, and we observed which permutations resulted in receptor switch or loss of function. Our results indicate that the determinants of receptor choice are found within the just 120 amino acids of SU proteins. Downstream sequences contribute to the stabilization of the receptor-specific structure.

This article has been cited by other articles:

• Sun, C., Zhang, B., Jin, J., Montelaro, R. C. (2008). Binding of equine infectious anemia virus to the equine lentivirus receptor-1 is mediated by complex discontinuous sequences in the viral envelope gp90 protein. J. Gen. Virol. 89: 2011-2019 [Abstract] [Full Text]  
• Yan, Y., Knoper, R. C., Kozak, C. A. (2007). Wild Mouse Variants of Envelope Genes of Xenotropic/Polytropic Mouse Gammaretroviruses and Their XPR1 Receptors Elucidate Receptor Determinants of Virus Entry. J. Virol. 81: 10550-10557 [Abstract] [Full Text]  
• Sarangi, A., Bupp, K., Roth, M. J. (2007). Identification of a retroviral receptor used by an Envelope protein derived by peptide library screening. Proc. Natl. Acad. Sci. USA 104: 11032-11037 [Abstract] [Full Text]  
• Dong, B., Kim, S., Hong, S., Das Gupta, J., Malathi, K., Klein, E. A., Ganem, D., DeRisi, J. L., Chow, S. A., Silverman, R. H. (2007). From the Cover: An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors. Proc. Natl. Acad. Sci. USA 104: 1655-1660 [Abstract] [Full Text]  
• Jones, K. S., Fugo, K., Petrow-Sadowski, C., Huang, Y., Bertolette, D. C., Lisinski, I., Cushman, S. W., Jacobson, S., Ruscetti, F. W. (2006). Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells.. J. Virol. 80: 8291-8302 [Abstract] [Full Text]  
• Evans, L. H., Lavignon, M., Peterson, K., Hasenkrug, K., Robertson, S., Malik, F., Virtaneva, K. (2006). In vivo interactions of ecotropic and polytropic murine leukemia viruses in mixed retrovirus infections.. J. Virol. 80: 4748-4757 [Abstract] [Full Text]  
• Cheng, H. H., Anderson, M. M., Hankenson, F. C., Johnston, L., Kotwaliwale, C. V., Overbaugh, J. (2006). Envelope Determinants for Dual-Receptor Specificity in Feline Leukemia Virus Subgroup A and T Variants. J. Virol. 80: 1619-1628 [Abstract] [Full Text]  
• Chang, K. W., Barsov, E. V., Ferris, A. L., Hughes, S. H. (2005). Mutations of a Residue within the Polyproline-Rich Region of Env Alter the Replication Rate and Level of Cytopathic Effects in Chimeric Avian Retroviral Vectors. J. Virol. 79: 10258-10267 [Abstract] [Full Text]  
• Manel, N., Battini, J.-L., Sitbon, M. (2005). Human T Cell Leukemia Virus Envelope Binding and Virus Entry Are Mediated by Distinct Domains of the Glucose Transporter GLUT1. J. Biol. Chem. 280: 29025-29029 [Abstract] [Full Text]  
• Wu, T., Yan, Y., Kozak, C. A. (2005). Rmcf2, a Xenotropic Provirus in the Asian Mouse Species Mus castaneus, Blocks Infection by Polytropic Mouse Gammaretroviruses. J. Virol. 79: 9677-9684 [Abstract] [Full Text]  
• Farrell, K. B., Eiden, M. V. (2005). Dissection of Gammaretroviral Receptor Function by Using Type III Phosphate Transporters as Models. J. Virol. 79: 9332-9336 [Abstract] [Full Text]  
• Burkhart, M. D., D'Agostino, P., Kayman, S. C., Pinter, A. (2005). Involvement of the C-Terminal Disulfide-Bonded Loop of Murine Leukemia Virus SU Protein in a Postbinding Step Critical for Viral Entry. J. Virol. 79: 7868-7876 [Abstract] [Full Text]  
• Urban, J. H., Schneider, R. M., Compte, M., Finger, C., Cichutek, K., Alvarez-Vallina, L., Buchholz, C. J. (2005). Selection of functional human antibodies from retroviral display libraries. Nucleic Acids Res 33: e35-e35 [Abstract] [Full Text]  
• Chandhasin, C., Coan, P. N., Levy, L. S. (2005). Subtle Mutational Changes in the SU Protein of a Natural Feline Leukemia Virus Subgroup A Isolate Alter Disease Spectrum. J. Virol. 79: 1351-1360 [Abstract] [Full Text]  
• Bahrami, S., Duch, M., Pedersen, F. S. (2004). Change of Tropism of SL3-2 Murine Leukemia Virus, Using Random Mutational Libraries. J. Virol. 78: 9343-9351 [Abstract] [Full Text]  
• Zavorotinskaya, T., Qian, Z., Franks, J., Albritton, L. M. (2004). A Point Mutation in the Binding Subunit of a Retroviral Envelope Protein Arrests Virus Entry at Hemifusion. J. Virol. 78: 473-481 [Abstract] [Full Text]  
• Lu, C.-W., Roth, M. J. (2003). Role of the Mutation Q252R in Activating Membrane Fusion in the Murine Leukemia Virus Surface Envelope Protein. J. Virol. 77: 10841-10849 [Abstract] [Full Text]  
• Dreja, H., Gros, L., Villard, S., Bachrach, E., Oates, A., Granier, C., Chardes, T., Mani, J.-C., Piechaczyk, M., Pelegrin, M. (2003). Monoclonal Antibody 667 Recognizes the Variable Region A Motif of the Ecotropic Retrovirus CasBrE Envelope Glycoprotein and Inhibits Env Binding to the Viral Receptor. J. Virol. 77: 10984-10993 [Abstract] [Full Text]  
• Zhang, Y., Rassa, J. C., deObaldia, M. E., Albritton, L. M., Ross, S. R. (2003). Identification of the Receptor Binding Domain of the Mouse Mammary Tumor Virus Envelope Protein. J. Virol. 77: 10468-10478 [Abstract] [Full Text]  
• Kubo, Y., Amanuma, H. (2003). Mutational analysis of the R peptide cleavage site of Moloney murine leukaemia virus envelope protein. J. Gen. Virol. 84: 2253-2257 [Abstract] [Full Text]  
• Rainey, G. J. A., Natonson, A., Maxfield, L. F., Coffin, J. M. (2003). Mechanisms of Avian Retroviral Host Range Extension. J. Virol. 77: 6709-6719 [Abstract] [Full Text]  
• Barnett, A. L., Wensel, D. L., Li, W., Fass, D., Cunningham, J. M. (2003). Structure and Mechanism of a Coreceptor for Infection by a Pathogenic Feline Retrovirus. J. Virol. 77: 2717-2729 [Abstract] [Full Text]  
• Faix, P. H., Feldman, S. A., Overbaugh, J., Eiden, M. V. (2002). Host Range and Receptor Binding Properties of Vectors Bearing Feline Leukemia Virus Subgroup B Envelopes Can Be Modulated by Envelope Sequences outside of the Receptor Binding Domain. J. Virol. 76: 12369-12375 [Abstract] [Full Text]  
• Lauring, A. S., Cheng, H. H., Eiden, M. V., Overbaugh, J. (2002). Genetic and Biochemical Analyses of Receptor and Cofactor Determinants for T-Cell-Tropic Feline Leukemia Virus Infection. J. Virol. 76: 8069-8078 [Abstract] [Full Text]  
• Lavillette, D., Marin, M., Ruggieri, A., Mallet, F., Cosset, F.-L., Kabat, D. (2002). The Envelope Glycoprotein of Human Endogenous Retrovirus Type W Uses a Divergent Family of Amino Acid Transporters/Cell Surface Receptors. J. Virol. 76: 6442-6452 [Abstract] [Full Text]  
• Farrell, K. B., Ting, Y.-T., Eiden, M. V. (2002). Fusion-Defective Gibbon Ape Leukemia Virus Vectors Can Be Rescued by Homologous but Not Heterologous Soluble Envelope Proteins. J. Virol. 76: 4267-4274 [Abstract] [Full Text]  
• Rothenberg, S. M., Olsen, M. N., Laurent, L. C., Crowley, R. A., Brown, P. O. (2001). Comprehensive Mutational Analysis of the Moloney Murine Leukemia Virus Envelope Protein. J. Virol. 75: 11851-11862 [Abstract] [Full Text]  
• Elrouby, N., Bureau, T. E. (2001). A Novel Hybrid Open Reading Frame Formed by Multiple Cellular Gene Transductions by a Plant Long Terminal Repeat Retroelement. J. Biol. Chem. 276: 41963-41968 [Abstract] [Full Text]  
• Lauring, A. S., Anderson, M. M., Overbaugh, J. (2001). Specificity in Receptor Usage by T-Cell-Tropic Feline Leukemia Viruses: Implications for the In Vivo Tropism of Immunodeficiency-Inducing Variants. J. Virol. 75: 8888-8898 [Abstract] [Full Text]  
• Barnett, A. L., Cunningham, J. M. (2001). Receptor Binding Transforms the Surface Subunit of the Mammalian C-Type Retrovirus Envelope Protein from an Inhibitor to an Activator of Fusion. J. Virol. 75: 9096-9105 [Abstract] [Full Text]  
• Overbaugh, J., Miller, A. D., Eiden, M. V. (2001). Receptors and Entry Cofactors for Retroviruses Include Single and Multiple Transmembrane-Spanning Proteins as well as Newly Described Glycophosphatidylinositol-Anchored and Secreted Proteins. Microbiol. Mol. Biol. Rev. 65: 371-389 [Abstract] [Full Text]  
• Sugai, J., Eiden, M., Anderson, M. M., Van Hoeven, N., Meiering, C. D., Overbaugh, J. (2001). Identification of Envelope Determinants of Feline Leukemia Virus Subgroup B That Permit Infection and Gene Transfer to Cells Expressing Human Pit1 or Pit2. J. Virol. 75: 6841-6849 [Abstract] [Full Text]  
• Prassolov, V., Hein, S., Ziegler, M., Ivanov, D., Münk, C., Löhler, J., Stocking, C. (2001). Mus cervicolor Murine Leukemia Virus Isolate M813 Belongs to a Unique Receptor Interference Group. J. Virol. 75: 4490-4498 [Abstract] [Full Text]  
• Lu, C.-W., Roth, M. J. (2001). Functional Characterization of the N Termini of Murine Leukemia Virus Envelope Proteins. J. Virol. 75: 4357-4366 [Abstract] [Full Text]  
• Lavillette, D., Boson, B., Russell, S. J., Cosset, F.-L. (2001). Activation of Membrane Fusion by Murine Leukemia Viruses Is Controlled in cis or in trans by Interactions between the Receptor-Binding Domain and a Conserved Disulfide Loop of the Carboxy Terminus of the Surface Glycoprotein. J. Virol. 75: 3685-3695 [Abstract] [Full Text]  
• Barnett, A. L., Davey, R. A., Cunningham, J. M. (2001). Modular organization of the Friend murine leukemia virus envelope protein underlies the mechanism of infection. Proc. Natl. Acad. Sci. USA 98: 4113-4118 [Abstract] [Full Text]  
• Bachrach, E., Marin, M., Pelegrin, M., Karavanas, G., Piechaczyk, M. (2000). Efficient Cell Infection by Moloney Murine Leukemia Virus-Derived Particles Requires Minimal Amounts of Envelope Glycoprotein. J. Virol. 74: 8480-8486 [Abstract] [Full Text]  
• Gwynn, S. R., Hankenson, F. C., Lauring, A. S., Rohn, J. L., Overbaugh, J. (2000). Feline Leukemia Virus Envelope Sequences That Affect T-Cell Tropism and Syncytium Formation Are Not Part of Known Receptor-Binding Domains. J. Virol. 74: 5754-5761 [Abstract] [Full Text]  
• Czauderna, F., Fischer, N., Boller, K., Kurth, R., Tönjes, R. R. (2000). Establishment and Characterization of Molecular Clones of Porcine Endogenous Retroviruses Replicating on Human Cells. J. Virol. 74: 4028-4038 [Abstract] [Full Text]  
• Tailor, C. S., Nouri, A., Kabat, D. (2000). A Comprehensive Approach to Mapping the Interacting Surfaces of Murine Amphotropic and Feline Subgroup B Leukemia Viruses with Their Cell Surface Receptors. J. Virol. 74: 237-244 [Abstract] [Full Text]  
• Lavillette, D., Ruggieri, A., Russell, S. J., Cosset, F.-L. (2000). Activation of a Cell Entry Pathway Common to Type C Mammalian Retroviruses by Soluble Envelope Fragments. J. Virol. 74: 295-304 [Abstract] [Full Text]  
• Askovic, S., McAtee, F. J., Favara, C., Portis, J. L. (2000). Brain Infection by Neuroinvasive but Avirulent Murine Oncornaviruses. J. Virol. 74: 465-473 [Abstract] [Full Text]  
• O'Reilly, L., Roth, M. J. (2000). Second-Site Changes Affect Viability of Amphotropic/Ecotropic Chimeric Enveloped Murine Leukemia Viruses. J. Virol. 74: 899-913 [Abstract] [Full Text]  
• Zavorotinskaya, T., Albritton, L. M. (1999). A Hydrophobic Patch in Ecotropic Murine Leukemia Virus Envelope Protein Is the Putative Binding Site for a Critical Tyrosine Residue on the Cellular Receptor. J. Virol. 73: 10164-10172 [Abstract] [Full Text]  
• Olsen, K. E. P., Andersen, K. B. (1999). Palmitoylation of the Intracytoplasmic R Peptide of the Transmembrane Envelope Protein in Moloney Murine Leukemia Virus. J. Virol. 73: 8975-8981 [Abstract] [Full Text]  
• Taylor, G. M., Sanders, D. A. (1999). The Role of the Membrane-spanning Domain Sequence in Glycoprotein-mediated Membrane Fusion. Mol. Biol. Cell 10: 2803-2815 [Abstract] [Full Text]  
• Lee, S., Zhao, Y., Anderson, W. F. (1999). Receptor-Mediated Moloney Murine Leukemia Virus Entry Can Occur Independently of the Clathrin-Coated-Pit-Mediated Endocytic Pathway. J. Virol. 73: 5994-6005 [Abstract] [Full Text]  
• Davey, R. A., Zuo, Y., Cunningham, J. M. (1999). Identification of a Receptor-Binding Pocket on the Envelope Protein of Friend Murine Leukemia Virus. J. Virol. 73: 3758-3763 [Abstract] [Full Text]  
• Tomonaga, K., Coffin, J. M. (1999). Structures of Endogenous Nonecotropic Murine Leukemia Virus (MLV) Long Terminal Repeats in Wild Mice: Implication for Evolution of MLVs. J. Virol. 73: 4327-4340 [Abstract] [Full Text]  
• Kayman, S. C., Park, H., Saxon, M., Pinter, A. (1999). The Hypervariable Domain of the Murine Leukemia Virus Surface Protein Tolerates Large Insertions and Deletions, Enabling Development of a Retroviral Particle Display System. J. Virol. 73: 1802-1808 [Abstract] [Full Text]  
• Battini, J.-L., Rasko, J. E. J., Miller, A. D. (1999). A human cell-surface receptor for xenotropic and polytropic murine leukemia viruses: Possible role in G protein-coupled signal transduction. Proc. Natl. Acad. Sci. USA 96: 1385-1390 [Abstract] [Full Text]  
• Tailor, C. S., Nouri, A., Lee, C. G., Kozak, C., Kabat, D. (1999). Cloning and characterization of a cell surface receptor for xenotropic and polytropic murine leukemia viruses. Proc. Natl. Acad. Sci. USA 96: 927-932 [Abstract] [Full Text]  
• Ting, Y.-T., Wilson, C. A., Farrell, K. B., Chaudry, G. J., Eiden, M. V. (1998). Simian Sarcoma-Associated Virus Fails To Infect Chinese Hamster Cells despite the Presence of Functional Gibbon Ape Leukemia Virus Receptors. J. Virol. 72: 9453-9458 [Abstract] [Full Text]  
• Lavillette, D., Maurice, M., Roche, C., Russell, S. J., Sitbon, M., Cosset, F.-L. (1998). A Proline-Rich Motif Downstream of the Receptor Binding Domain Modulates Conformation and Fusogenicity of Murine Retroviral Envelopes. J. Virol. 72: 9955-9965 [Abstract] [Full Text]  
• Takeuchi, Y., Patience, C., Magre, S., Weiss, R. A., Banerjee, P. T., Le Tissier, P., Stoye, J. P. (1998). Host Range and Interference Studies of Three Classes of Pig Endogenous Retrovirus. J. Virol. 72: 9986-9991 [Abstract] [Full Text]  
• Han, J.-Y., Zhao, Y., Anderson, W. F., Cannon, P. M. (1998). Role of Variable Regions A and B in Receptor Binding Domain of Amphotropic Murine Leukemia Virus Envelope Protein. J. Virol. 72: 9101-9108 [Abstract] [Full Text]  
• Tomonaga, K., Coffin, J. M. (1998). Structure and Distribution of Endogenous Nonecotropic Murine Leukemia Viruses in Wild Mice. J. Virol. 72: 8289-8300 [Abstract] [Full Text]  
• Wolgamot, G., Bonham, L., Miller, A. D. (1998). Sequence Analysis of Mus dunni Endogenous Virus Reveals a Hybrid VL30/Gibbon Ape Leukemia Virus-Like Structure and a Distinct Envelope. J. Virol. 72: 7459-7466 [Abstract] [Full Text]  
• Weimin Wu, B., Cannon, P. M., Gordon, E. M., Hall, F. L., Anderson, W. F. (1998). Characterization of the Proline-Rich Region of Murine Leukemia Virus Envelope Protein. J. Virol. 72: 5383-5391 [Abstract] [Full Text]  
• Ott, M., Stockert, R. J., Ma, Q., Gagandeep, S., Gupta, S. (1998). Simultaneous Up-regulation of Viral Receptor Expression and DNA Synthesis Is Required for Increasing Efficiency of Retroviral Hepatic Gene Transfer. J. Biol. Chem. 273: 11954-11961 [Abstract] [Full Text]  
• Lundorf, M. D., Pedersen, F. S., O'Hara, B., Pedersen, L. (1998). Single Amino Acid Insertion in Loop 4 Confers Amphotropic Murine Leukemia Virus Receptor Function upon Murine Pit1. J. Virol. 72: 4524-4527 [Abstract] [Full Text]  
• Fielding, A. K., Maurice, M., Morling, F. J., Cosset, F.-L., Russell, S. J. (1998). Inverse Targeting of Retroviral Vectors: Selective Gene Transfer in a Mixed Population of Hematopoietic and Nonhematopoietic Cells. Blood 91: 1802-1809 [Abstract] [Full Text]  
• Battini, J. L., Danos, O., Heard, J. M. (1998). Definition of a 14-Amino-Acid Peptide Essential for the Interaction between the Murine Leukemia Virus Amphotropic Envelope Glycoprotein and Its Receptor. J. Virol. 72: 428-435 [Abstract] [Full Text]  
• Fass, D., Davey, R. A., Hamson, C. A., Kim, P. S., Cunningham, J. M., Berger, J. M. (1997). Structure of a Murine Leukemia Virus Receptor-Binding Glycoprotein at 2.0 Angstrom Resolution. Science 277: 1662-1666 [Abstract] [Full Text]  
• Schnierle, B. S., Stitz, J., Bosch, V., Nocken, F., Merget-Millitzer, H., Engelstadter, M., Kurth, R., Groner, B., Cichutek, K. (1997). Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells. Proc. Natl. Acad. Sci. USA 94: 8640-8645 [Abstract] [Full Text]  
• Kim, F. J., Seiliez, I., Denesvre, C., Lavillette, D., Cosset, F.-L., Sitbon, M. (2000). Definition of an Amino-terminal Domain of the Human T-cell Leukemia Virus Type 1 Envelope Surface Unit That Extends the Fusogenic Range of an Ecotropic Murine Leukemia Virus. J. Biol. Chem. 275: 23417-23420 [Abstract] [Full Text]