This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dropulic, B Articles by Jeang, K T Search for Related Content PubMed PubMed Citation Articles by Dropulic, B Articles by Jeang, K T

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression.

B Dropuli, N H Lin, M A Martin and K T Jeang

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

ABSTRACT

We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication was suppressed in tissue culture. Introduction of this ribozyme into cells by using an amphotropic retrovirus vector significantly reduced expression of U5-containing RNA in cells chronically infected with HIV-1. Naive T cells were cocultivated with packaging cells that produce defective amphotropic retroviruses containing the U5 ribozyme. These lymphocytes were found to be partially protected from HIV-1 infection.

This article has been cited by other articles:

• Morris, K. V., Grahn, R. A., Looney, D. J., Pedersen, N. C. (2004). Characterization of a mobilization-competent simian immunodeficiency virus (SIV) vector containing a ribozyme against SIV polymerase. J. Gen. Virol. 85: 1489-1496 [Abstract] [Full Text]  
• Shahi, S., Shanmugasundaram, G. K., Banerjea, A. C. (2001). Ribozymes that cleave reovirus genome segment S1 also protect cells from pathogenesis caused by reovirus infection. Proc. Natl. Acad. Sci. USA 10.1073/pnas.051013898v1 [Abstract] [Full Text]  
• Bramlage, B., Luzi, E., Eckstein, F. (2000). HIV-1 LTR as a target for synthetic ribozyme-mediated inhibition of gene expression: site selection and inhibition in cell culture. Nucleic Acids Res 28: 4059-4067 [Abstract] [Full Text]  
• Bunnell, B. A., Morgan, R. A. (1998). Gene Therapy for Infectious Diseases. Clin. Microbiol. Rev. 11: 42-56 [Abstract] [Full Text]  
• Gavin, D. K., Gupta, K. C. (1997). Efficient Hammerhead Ribozymes Targeted to the Polycistronic Sendai Virus P/C mRNA. STRUCTURE-FUNCTION RELATIONSHIPS. J. Biol. Chem. 272: 1461-1472 [Abstract] [Full Text]  
• Uchiyama, H., Hirano, K.'i., Kashiwasake-Jibu, M., Taira, K. (1996). Detection of Undegraded Oligonucleotides in Vivo by Fluorescence Resonance Energy Transfer. J. Biol. Chem. 271: 380-384 [Abstract] [Full Text]  
• Shahi, S., Shanmugasundaram, G. K., Banerjea, A. C. (2001). Ribozymes that cleave reovirus genome segment S1 also protect cells from pathogenesis caused by reovirus infection. Proc. Natl. Acad. Sci. USA 98: 4101-4106 [Abstract] [Full Text]