This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rogel-Gaillard, C Articles by Orth, G Search for Related Content PubMed PubMed Citation Articles by Rogel-Gaillard, C Articles by Orth, G

 Previous Article  |  Next Article 

J Virol. 1992 February; 66(2): 816-823

Human papillomavirus type 1 E4 proteins differing by their N-terminal ends have distinct cellular localizations when transiently expressed in vitro.

Unité des Papillomavirus, Institut Pasteur, Paris, France.

ABSTRACT

Two major human papillomavirus type 1 (HPV-1) E4 proteins are found in large amounts in productively infected differentiating wart cells, a 17-kDa protein translated from an E1-E4 transcript and a processed 16-kDa protein lacking the E1 amino acids at least. The functions of the E4 proteins are still unknown. We have designed an in vitro system allowing the transient expression of three forms of HPV-1 E4 proteins: the 17-kDa E1-E4 protein, an E4 protein without the five E1 amino acids (E4-3200), and E4 protein initiated at the E4 ATG located upstream of the splice acceptor site (E4-3181). The E4-3181 protein has five additional N-terminal amino acids compared with E4-3200. The E4-3181 protein has not yet been detected in vivo but could, in principle, be translated from any transcript encoding the E2 protein. The constructs were transfected in two keratinocyte lines, one derived from a rabbit transplantable carcinoma (VX2R) and the other from a human penile carcinoma (SK-p). E4 transcripts with the expected size were detected in both cell lines by Northern (RNA) blot analysis. Surprisingly, the E4 proteins were found only in the VX2R cells by radioimmunoprecipitation and immunofluorescence experiments. The E1-E4 and the E4-3200 proteins were both cytoplasmic and were associated with granules reminiscent of the cytoplasmic inclusions pathognomonic of the HPV-1 infection. Moreover, each protein showed a specific staining pattern of the inclusions. In contrast, the E4-3181 protein was essentially intranuclear and perinuclear. Thus, HPV-1 E4 proteins differing in their N-terminal ends have distinct cellular localizations and arrangements. It is tempting to assume that this may relate to different roles.

This article has been cited by other articles:

• Knight, G. L., Grainger, J. R., Gallimore, P. H., Roberts, S. (2004). Cooperation between Different Forms of the Human Papillomavirus Type 1 E4 Protein To Block Cell Cycle Progression and Cellular DNA Synthesis. J. Virol. 78: 13920-13933 [Abstract] [Full Text]  
• Nakahara, T., Nishimura, A., Tanaka, M., Ueno, T., Ishimoto, A., Sakai, H. (2002). Modulation of the Cell Division Cycle by Human Papillomavirus Type 18 E4. J. Virol. 76: 10914-10920 [Abstract] [Full Text]  
• Peh, W. L., Middleton, K., Christensen, N., Nicholls, P., Egawa, K., Sotlar, K., Brandsma, J., Percival, A., Lewis, J., Liu, W. J., Doorbar, J. (2002). Life Cycle Heterogeneity in Animal Models of Human Papillomavirus-Associated Disease. J. Virol. 76: 10401-10416 [Abstract] [Full Text]  
• Doorbar, J., Elston, R. C., Napthine, S., Raj, K., Medcalf, E., Jackson, D., Coleman, N., Griffin, H. M., Masterson, P., Stacey, S., Mengistu, Y., Dunlop, J. (2000). The E1and E4 Protein of Human Papillomavirus Type 16 Associates with a Putative RNA Helicase through Sequences in Its C Terminus. J. Virol. 74: 10081-10095 [Abstract] [Full Text]  
• Petit, M. M. R., Fradelizi, J., Golsteyn, R. M., Ayoubi, T. A.Y., Menichi, B., Louvard, D., Van de Ven, W. J. M., Friederich, E. (2000). LPP, an Actin Cytoskeleton Protein Related to Zyxin, Harbors a Nuclear Export Signal and Transcriptional Activation Capacity. Mol. Biol. Cell 11: 117-129 [Abstract] [Full Text]  
• Eriksson, A, Herron, J., Yamada, T, Wheeler, C. (1999). Human papillomavirus type 16 variant lineages characterized by nucleotide sequence analysis of the E5 coding segment and the E2 hinge region. J. Gen. Virol. 80: 595-600 [Abstract]  
• Golsteyn, R., Beckerle, M., Koay, T, Friederich, E (1997). Structural and functional similarities between the human cytoskeletal protein zyxin and the ActA protein of Listeria monocytogenes. J. Cell Sci. 110: 1893-1906 [Abstract]