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J Virol. 1992 February; 66(2): 1273-1276

Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood.

T F Schwarz, S Serke, B Hottenträger, A von Brunn, H Baurmann, A Kirsch, W Stolz, D Huhn, F Deinhardt and M Roggendorf

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilian University, Munich, Germany.

ABSTRACT

Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.


J Virol. 1992 February; 66(2): 1273-1276




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Copyright © 1992 by the American Society for Microbiology. All rights reserved.