This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kralli, A Articles by Weinmann, R Search for Related Content PubMed PubMed Citation Articles by Kralli, A Articles by Weinmann, R

 Previous Article  |  Next Article 

J Virol. 1992 December; 66(12): 6979-6988

Negative regulation of the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells via a retinoic acid response element.

Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.

ABSTRACT

In cells transformed by the highly oncogenic adenovirus type 12 (Ad12), the viral E1A proteins mediate transcriptional repression of the major histocompatibility class I genes. In contrast, class I transcription is not reduced in cells transformed by the nononcogenic Ad5. The decreased rate of class I transcription is, at least in part, the result of a reduced major histocompatibility complex class I enhancer activity in Ad12-transformed cells and correlates with an increase in the levels of a DNA-binding activity to the R2 element of the enhancer (R. Ge, A. Kralli, R. Weinmann, and R. P. Ricciardi, J. Virol. 66:6969-6978, 1992). Employing transient transfection assays, we now provide direct evidence that the R2 element can confer repression in Ad12- but not Ad5-transformed cells. Repression by R2 was observed only in the presence of the positive enhancer element R1 and was dependent on (i) the number of the R2 elements and (ii) the relative arrangement of R2 and R1 elements. The putative R2-binding repressor protein, R2BF, was similar in molecular weight and binding specificity to members of the thyroid hormone/retinoic acid (RA) receptor family. RA treatment abrogated the R2-mediated repression in Ad12-transformed cells and had no effect on the activity of R2/R1-containing promoters in Ad5-transformed cells. These results are consistent with the presence of an R2-binding repressor in Ad12-transformed cells. In the absence of RA, the repressor compromises enhancer activity by interfering with the activity of the positive cis element R1. RA treatment of Ad12-transformed cells may render the repressor inactive.

This article has been cited by other articles:

• Guan, H., Hou, S., Ricciardi, R. P. (2005). DNA Binding of Repressor Nuclear Factor-{kappa}B p50/p50 Depends on Phosphorylation of Ser337 by the Protein Kinase A Catalytic Subunit. J. Biol. Chem. 280: 9957-9962 [Abstract] [Full Text]  
• Hou, S., Guan, H., Ricciardi, R. P. (2003). Phosphorylation of Serine 337 of NF-{kappa}B p50 Is Critical for DNA Binding. J. Biol. Chem. 278: 45994-45998 [Abstract] [Full Text]  
• Hou, S., Guan, H., Ricciardi, R. P. (2002). In Adenovirus Type 12 Tumorigenic Cells, Major Histocompatibility Complex Class I Transcription Shutoff Is Overcome by Induction of NF-{kappa}B and Relief of COUP-TFII Repression. J. Virol. 76: 3212-3220 [Abstract] [Full Text]  
• Graeven, U., Rodeck, U., Karpinski, S., Jost, M., Philippou, S., Schmiegel, W. (2001). Modulation of Angiogenesis and Tumorigenicity of Human Melanocytic Cells by Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor. Cancer Res. 61: 7282-7290 [Abstract] [Full Text]  
• Vallejo, A. N., Pease, L. R. (1996). The Locus-specific Enhancer Activity of the Class I Major Histocompatibility Complex Interferon-responsive Element Is Associated with a gamma -Interferon (IFN)-inducible Factor Distinct from STAT1alpha , p48, and IFN Regulatory Factor-1. J. Biol. Chem. 271: 29813-29821 [Abstract] [Full Text]  
• Tang, X., Li, H.-O., Sakatsume, O., Ohta, T., Tsutsui, H., Smit, A. F. A., Horikoshi, M., Kourilsky, P., Israël, A., Gachelin, G., Yokoyama, K. (1995). Cooperativity between an Upstream TATA-like Sequence and a CAA Repeated Element Mediates E1A-dependent Negative Repression of the H-2K^b Class I Gene. J. Biol. Chem. 270: 2327-2336 [Abstract] [Full Text]