This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morrey, J D Articles by Rosen, C A Search for Related Content PubMed PubMed Citation Articles by Morrey, J D Articles by Rosen, C A

 Previous Article  |  Next Article 

J Virol. 1991 September; 65(9): 5045-5051

In vivo activation of human immunodeficiency virus type 1 long terminal repeat by UV type A (UV-A) light plus psoralen and UV-B light in the skin of transgenic mice.

J D Morrey, S M Bourn, T D Bunch, M K Jackson, R W Sidwell, L R Barrows, R A Daynes and C A Rosen

AIDS Research Program, Utah State University, Logan 84322-5600.

ABSTRACT

UV irradiation has been shown to activate the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in cell culture; however, only limited studies have been described in vivo. UV light has been categorized as UV-A (400 to 315 nm), -B (315 to 280 nm), or -C (less than 280 nm); the longer wavelengths are less harmful but more penetrative. Highly penetrative UV-A radiation constitutes the vast majority of UV sunlight reaching the earth's surface but is normally harmless. UV-B irradiation is more harmful but less prevalent than UV-A. In this report, the HIV-1 LTR-luciferase gene in the skin of transgenic mice was markedly activated when exposed to UV-B irradiation. The LTR in the skin of transgenic mice pretreated topically with a photosensitizing agent (psoralen) was also activated to similar levels when exposed to UV-A light. A 2-h exposure to sunlight activated the LTR in skin treated with psoralen, whereas the LTR in skin not treated with psoralen was activated after 7 h of sunlight exposure. The HIV-1 LTR-beta-galactosidase reporter gene was preferentially activated by UV-B irradiation in a small population of epidermal cells. The transgenic mouse models carrying HIV-1 LTR-luciferase and LTR-beta-galactosidase reporter genes have been used to demonstrate the in vivo UV-induced activation of the LTR and might be used to evaluate other environmental factors or pharmacologic substances that might potentially activate the HIV-1 LTR in vivo.

---

J Virol. 1991 September; 65(9): 5045-5051

This article has been cited by other articles:

• Gelfand, J. M., Rudikoff, D., Lebwohl, M., Klotman, M. E. (1998). Effect of UV-B Phototherapy on Plasma HIV Type 1 RNA Viral Level: A Self-controlled Prospective Study. Arch Dermatol 134: 940-945 [Abstract] [Full Text]  
• Buccheri, L., Katchen, B. R., Karter, A. J., Cohen, S. R. (1997). Acitretin Therapy Is Effective for Psoriasis Associated With Human Immunodeficiency Virus Infection. Arch Dermatol 133: 711-715 [Abstract]  
• Duvic, M., Crane, M. M., Conant, M., Mahoney, S. E., Reveille, J. D., Lehrman, S. N. (1994). Zidovudine Improves Psoriasis in Human Immunodeficiency Virus--Positive Males. Arch Dermatol 130: 447-451 [Abstract]  
• Vernet, M., Cavard, C., Zider, A., Fergelot, P., Grimber, G., Briand, P. (1993). In vitro manipulation of early mouse embryos induces HIV1-LTRlacZ transgene expression. Development 119: 1293-1300 [Abstract]  
• Wallace, B., Lasker, J. (1992). Awakenings ... UV light and HIV gene activation. Science 257: 1211-1212