This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carpenter, M S Articles by DeLange, A M Search for Related Content PubMed PubMed Citation Articles by Carpenter, M S Articles by DeLange, A M

 Previous Article  |  Next Article 

J Virol. 1991 August; 65(8): 4042-4050

A temperature-sensitive lesion in the small subunit of the vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution.

Department of Human Genetics, University of Manitoba, Winnipeg, Canada.

ABSTRACT

Using pulsed-field gel electrophoresis, we demonstrated that the temperature-sensitive (ts) conditional lethal mutant ts9383 is, at the nonpermissive temperature, defective in the resolution of concatemeric replicative intermediate DNA to linear 185-kb monomeric DNA genomes. The resolution defect was shown to be the result of a partial failure of the mutant virus to convert the replicated form of the viral telomere to hairpin termini. In contrast to other mutants of this phenotype, pulse-labeling of viral proteins at various times postinfection revealed no obvious difference in the quantity or temporal appearance of members of the late class of polypeptides. Using the marker rescue technique, we localized the ts lesion in ts9383 to an approximately 1-kb region within the HindIII D fragment. Both the ts phenotype and the resolution defect were shown to be caused by a single-base C----T point mutation resulting in the conversion of the amino acid proline to serine in codon 23 of open reading frame D12. This gene encodes a 33-kDa polypeptide which is known to be the small subunit of the virus-encoded mRNA capping enzyme (E. G. Niles, G. J. Lee-Chen, S. Shuman, B. Moss, and S. S. Broyles, Virology 172:513-522, 1989). The data are consistent with a role for this capping enzyme subunit during poxviral telomere resolution.

This article has been cited by other articles:

• Schwer, B., Hausmann, S., Schneider, S., Shuman, S. (2006). Poxvirus mRNA Cap Methyltransferase: BYPASS OF THE REQUIREMENT FOR THE STIMULATORY SUBUNIT BY MUTATIONS IN THE CATALYTIC SUBUNIT AND EVIDENCE FOR INTERSUBUNIT ALLOSTERY. J. Biol. Chem. 281: 18953-18960 [Abstract] [Full Text]  
• Saha, N., Shuman, S., Schwer, B. (2003). Yeast-Based Genetic System for Functional Analysis of Poxvirus mRNA Cap Methyltransferase. J. Virol. 77: 7300-7307 [Abstract] [Full Text]  
• Garcia, A. D., Moss, B. (2001). Repression of Vaccinia Virus Holliday Junction Resolvase Inhibits Processing of Viral DNA into Unit-Length Genomes. J. Virol. 75: 6460-6471 [Abstract] [Full Text]  
• Ho, C. K., Martins, A., Shuman, S. (2000). A Yeast-Based Genetic System for Functional Analysis of Viral mRNA Capping Enzymes. J. Virol. 74: 5486-5494 [Abstract] [Full Text]