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J Virol. 1991 May; 65(5): 2237-2244

Pathways of activation of the Epstein-Barr virus productive cycle.

Ludwig Institute for Cancer Research, St. Mary's Hospital Medical School, Paddington, London, England.

ABSTRACT

The promoter for the 2.8-kb RNA of Epstein-Barr virus encoding BZLF1 and BRLF1 was identified and shown to be activated by both BZLF1 and BRLF1 but not by 12-O-tetradecanoylphorbol-13-acetate. Site-directed mutagenesis suggests that two binding sites for BZLF1 within the promoter contribute to the transactivation by BZLF1. The early kinetics of induction of the 2.8- and 1.0-kb RNAs encoding BZLF1 and BRLF1 in Akata cells treated with anti-immunoglobulin indicate that both RNAs appear within 60 min. The results indicate some likely pathways of activation of Epstein-Barr virus productive cycle gene expression.

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• Darr, C. D., Mauser, A., Kenney, S. (2001). Epstein-Barr Virus Immediate-Early Protein BRLF1 Induces the Lytic Form of Viral Replication through a Mechanism Involving Phosphatidylinositol-3 Kinase Activation. J. Virol. 75: 6135-6142 [Abstract] [Full Text]  
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• Liu, S., Pavlova, I. V., Virgin, H. W. IV, Speck, S. H. (2000). Characterization of Gammaherpesvirus 68 Gene 50 Transcription. J. Virol. 74: 2029-2037 [Abstract] [Full Text]  
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• Ragoczy, T., Miller, G. (1999). Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes. J. Virol. 73: 9858-9866 [Abstract] [Full Text]  
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• Semmes, O. J., Chen, L., Sarisky, R. T., Gao, Z., Zhong, L., Hayward, S. D. (1998). Mta Has Properties of an RNA Export Protein and Increases Cytoplasmic Accumulation of Epstein-Barr Virus Replication Gene mRNA. J. Virol. 72: 9526-9534 [Abstract] [Full Text]  
• Ragoczy, T., Heston, L., Miller, G. (1998). The Epstein-Barr Virus Rta Protein Activates Lytic Cycle Genes and Can Disrupt Latency in B Lymphocytes. J. Virol. 72: 7978-7984 [Abstract] [Full Text]  
• Zacny, V. L., Wilson, J., Pagano, J. S. (1998). The Epstein-Barr Virus Immediate-Early Gene Product, BRLF1, Interacts with the Retinoblastoma Protein during the Viral Lytic Cycle. J. Virol. 72: 8043-8051 [Abstract] [Full Text]  
• Chang, P.-J., Chang, Y.-S., Liu, S.-T. (1998). Role of Rta in the Translation of Bicistronic BZLF1 of Epstein-Barr Virus. J. Virol. 72: 5128-5136 [Abstract] [Full Text]  
• Garcia-Blanco, M., Cullen, B. (1991). Molecular basis of latency in pathogenic human viruses. Science 254: 815-820 [Abstract]