This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Remington, K M Articles by North, T W Search for Related Content PubMed PubMed Citation Articles by Remington, K M Articles by North, T W

 Previous Article  |  Next Article 

J Virol. 1991 January; 65(1): 308-312

Mutants of feline immunodeficiency virus resistant to 3'-azido-3'-deoxythymidine.

Division of Biological Sciences, University of Montana, Missoula 59812.

ABSTRACT

We selected 3'-azido-3'-deoxythymidine (AZT)-resistant mutants of feline immunodeficiency virus (FIV) in a cat cell culture system. The characterization of one of these mutants was facilitated by the development of a focal immunoassay which could accurately measure FIV infectivity. This assay was used to quantitate the susceptibility of FIV to various inhibitors. The AZT-resistant mutant was found to be cross-resistant to 3'-azido-2',3'-dideoxyuridine and 3'-azido-2',3'-dideoxyguanosine but remained sensitive to several other inhibitors (2',3'-dideoxyinosine, 2',3'-dideoxy-2',3'-didehydrothymidine, and phosphonoformate). These patterns of cross-resistance and sensitivity were similar to those of the AZT-resistant human immunodeficiency virus (HIV) that has recently been isolated from patients with AIDS (B. A. Larder and S. D. Kemp, Science 246:1155-1158, 1989). Like the AZT-resistant HIV, purified reverse transcriptase from mutant FIV failed to show resistance to the 5'-triphosphate of AZT. This mutant can be used in the FIV model system to study the mechanisms of drug resistance and to determine the pathogenicity of AZT-resistant mutants.

This article has been cited by other articles:

• Saenz, D. T., Loewen, N., Peretz, M., Whitwam, T., Barraza, R., Howell, K. G., Holmes, J. M., Good, M., Poeschla, E. M. (2004). Unintegrated Lentivirus DNA Persistence and Accessibility to Expression in Nondividing Cells: Analysis with Class I Integrase Mutants. J. Virol. 78: 2906-2920 [Abstract] [Full Text]  
• Murry, J. P., Higgins, J., Matthews, T. B., Huang, V. Y., Van Rompay, K. K. A., Pedersen, N. C., North, T. W. (2002). Reversion of the M184V Mutation in Simian Immunodeficiency Virus Reverse Transcriptase Is Selected by Tenofovir, Even in the Presence of Lamivudine. J. Virol. 77: 1120-1130 [Abstract] [Full Text]  
• Whitwam, T., Peretz, M., Poeschla, E. (2001). Identification of a Central DNA Flap in Feline Immunodeficiency Virus. J. Virol. 75: 9407-9414 [Abstract] [Full Text]  
• Klarmann, G. J., Smith, R. A., Schinazi, R. F., North, T. W., Preston, B. D. (2000). Site-specific Incorporation of Nucleoside Analogs by HIV-1 Reverse Transcriptase and the Template Grip Mutant P157S. TEMPLATE INTERACTIONS INFLUENCE SUBSTRATE RECOGNITION AT THE POLYMERASE ACTIVE SITE. J. Biol. Chem. 275: 359-366 [Abstract] [Full Text]  
• Poeschla, E. M., Looney, D. J. (1998). CXCR4 Is Required by a Nonprimate Lentivirus: Heterologous Expression of Feline Immunodeficiency Virus in Human, Rodent, and Feline Cells. J. Virol. 72: 6858-6866 [Abstract] [Full Text]  
• Smith, R. A., Remington, K. M., Preston, B. D., Schinazi, R. F., North, T. W. (1998). A Novel Point Mutation at Position 156 of Reverse Transcriptase from Feline Immunodeficiency Virus Confers Resistance to the Combination of (-)-beta -2',3'-Dideoxy-3'-Thiacytidine and 3'-Azido-3'-Deoxythymidine. J. Virol. 72: 2335-2340 [Abstract] [Full Text]