This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kannagi, M Articles by Harada, S Search for Related Content PubMed PubMed Citation Articles by Kannagi, M Articles by Harada, S

Interference with human immunodeficiency virus (HIV) replication by CD8+ T cells in peripheral blood leukocytes of asymptomatic HIV carriers in vitro.

Department of Biodefence and Medical Virology, Kumamoto University Medical School, Japan.

ABSTRACT

A long asymptomatic period is one of the characteristics of human immunodeficiency virus (HIV) infection, despite its fatal consequences. Antiviral defense in HIV-infected individuals controls viral replication during this period. In the present study, we demonstrate that peripheral blood leukocytes (PBL) of asymptomatic HIV-1 carriers, following exogenous HIV-1 infection in vitro, do not support viral replication. These cells do not produce detectable amounts of reverse transcriptase or accumulate unintegrated proviral DNA. This is a striking contrast to the behavior of HIV-1-infected PBL of seronegative individuals, which produce large amounts of RT and unintegrated DNA. Such resistance to HIV-1 replication is not seen in PBL of patients with advanced disease. Since the binding of HIV-1 to CD4 molecule is not impaired in PBL of asymptomatic carriers, the interference with HIV replication must occur after the stage of virus binding. PBL lose their resistance when CD8+ lymphocytes are removed. In addition, these PBL are not resistant to an exogenous infection with HIV-2. These observations suggest that certain populations of CD8+ lymphocytes of asymptomatic HIV-1 carriers operate on the target cells in PBL to block viral replication in an HIV-1-specific manner. Such CD8+ lymphocyte-mediated interference with HIV replication could play an important role in the maintenance of the period of disease latency.

This article has been cited by other articles:

• Kubo, M., Nishitsuji, H., Kurihara, K., Hayashi, T., Masuda, T., Kannagi, M. (2006). Suppression of human immunodeficiency virus type 1 replication by arginine deiminase of Mycoplasma arginini. J. Gen. Virol. 87: 1589-1593 [Abstract] [Full Text]  
• Quinones-Mateu, M. E., Gao, Y., Ball, S. C., Marozsan, A. J., Abraha, A., Arts, E. J. (2002). In Vitro Intersubtype Recombinants of Human Immunodeficiency Virus Type 1: Comparison to Recent and Circulating In Vivo Recombinant Forms. J. Virol. 76: 9600-9613 [Abstract] [Full Text]  
• Gauduin, M.-C., Glickman, R. L., Means, R., Johnson, R. P. (1998). Inhibition of Simian Immunodeficiency Virus (SIV) Replication by CD8+ T Lymphocytes from Macaques Immunized with Live Attenuated SIV. J. Virol. 72: 6315-6324 [Abstract] [Full Text]  
• Moriuchi, H., Moriuchi, M., Combadiere, C., Murphy, P. M., Fauci, A. S. (1996). CD8+ T-cell-derived soluble factor(s), but not beta -chemokines RANTES, MIP-1alpha , and MIP-1beta , suppress HIV-1 replication in monocyte/macrophages. Proc. Natl. Acad. Sci. USA 93: 15341-15345 [Abstract] [Full Text]  
• Travers, K, Mboup, S, Marlink, R, Gueye-Nidaye, A, Siby, T, Thior, I, Traore, I, Dieng-Sarr, A, Sankale, J., Mullins, C, et, al. (1995). Natural protection against HIV-1 infection provided by HIV-2. Science 268: 1612-1615 [Abstract]