This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, A J
Right arrow Articles by Rekosh, D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, A J
Right arrow Articles by Rekosh, D

 Previous Article  |  Next Article 

J Virol. 1990 June; 64(6): 2743-2750

Human immunodeficiency virus type 1 Pr55gag and Pr160gag-pol expressed from a simian virus 40 late replacement vector are efficiently processed and assembled into viruslike particles.

A J Smith, M I Cho, M L Hammarskjöld and D Rekosh

Department of Microbiology, State University of New York, Buffalo 14214.

ABSTRACT

Human immunodeficiency virus type 1 (HIV-1) gag and pol genes were expressed by using fragments of the BH10 clone of HIV inserted into a simian virus 40 late replacement vector. An initial construct containing the entire coding regions of gag, pol, and vif produced only minute amounts of the gag precursor, Pr55gag. However, high-level expression was obtained when an additional sequence from the env gene (the rev-responsive element) was inserted 3' of vif in the correct orientation, and rev was provided in trans from a second vector. Western immunoblot analysis of transfected cells showed the presence of large amounts of both Pr55gag and Pr160gag-pol as well as all of the expected cleavage products. Electron microscopy of thin sections of transfected cells showed a multitude of viruslike particles. Both immature particles in the process of budding and particles containing the condensed core characteristic of HIV were observed. Analysis of the released viruslike particles showed the presence of active reverse transcriptase. Sucrose gradient analysis of particles produced from [3H]uridine-labeled cells indicated a peak of radioactivity which cosedimented with a peak of p24, suggesting that the particles contained RNA.

J Virol. 1990 June; 64(6): 2743-2750




This article has been cited by other articles:

  • Li, H., Chien, P. C. Jr., Tuen, M., Visciano, M. L., Cohen, S., Blais, S., Xu, C.-F., Zhang, H.-T., Hioe, C. E. (2008). Identification of an N-Linked Glycosylation in the C4 Region of HIV-1 Envelope gp120 That Is Critical for Recognition of Neighboring CD4 T Cell Epitopes. J. Immunol. 180: 4011-4021 [Abstract] [Full Text]  
  • Bouamr, F., Houck-Loomis, B. R., De Los Santos, M., Casaday, R. J., Johnson, M. C., Goff, S. P. (2007). The C-Terminal Portion of the Hrs Protein Interacts with Tsg101 and Interferes with Human Immunodeficiency Virus Type 1 Gag Particle Production. J. Virol. 81: 2909-2922 [Abstract] [Full Text]  
  • Ciancanelli, M. J., Basler, C. F. (2006). Mutation of YMYL in the Nipah Virus Matrix Protein Abrogates Budding and Alters Subcellular Localization. J. Virol. 80: 12070-12078 [Abstract] [Full Text]  
  • Lingappa, J. R., Dooher, J. E., Newman, M. A., Kiser, P. K., Klein, K. C. (2006). Basic Residues in the Nucleocapsid Domain of Gag Are Required for Interaction of HIV-1 Gag with ABCE1 (HP68), a Cellular Protein Important for HIV-1 Capsid Assembly. J. Biol. Chem. 281: 3773-3784 [Abstract] [Full Text]  
  • Guo, F., Gabor, J., Cen, S., Hu, K., Mouland, A. J., Kleiman, L. (2005). Inhibition of Cellular HIV-1 Protease Activity by Lysyl-tRNA Synthetase. J. Biol. Chem. 280: 26018-26023 [Abstract] [Full Text]  
  • Halwani, R., Cen, S., Javanbakht, H., Saadatmand, J., Kim, S., Shiba, K., Kleiman, L. (2004). Cellular Distribution of Lysyl-tRNA Synthetase and Its Interaction with Gag during Human Immunodeficiency Virus Type 1 Assembly. J. Virol. 78: 7553-7564 [Abstract] [Full Text]  
  • Alexander, M., Bor, Y.-c., Ravichandran, K. S., Hammarskjold, M.-L., Rekosh, D. (2004). Human Immunodeficiency Virus Type 1 Nef Associates with Lipid Rafts To Downmodulate Cell Surface CD4 and Class I Major Histocompatibility Complex Expression and To Increase Viral Infectivity. J. Virol. 78: 1685-1696 [Abstract] [Full Text]  
  • Lu, R., Nakajima, N., Hofmann, W., Benkirane, M., Teh-Jeang, K., Sodroski, J., Engelman, A. (2004). Simian Virus 40-Based Replication of Catalytically Inactive Human Immunodeficiency Virus Type 1 Integrase Mutants in Nonpermissive T Cells and Monocyte-Derived Macrophages. J. Virol. 78: 658-668 [Abstract] [Full Text]  
  • Cen, S., Niu, M., Saadatmand, J., Guo, F., Huang, Y., Nabel, G. J., Kleiman, L. (2004). Incorporation of Pol into Human Immunodeficiency Virus Type 1 Gag Virus-Like Particles Occurs Independently of the Upstream Gag Domain in Gag-Pol. J. Virol. 78: 1042-1049 [Abstract] [Full Text]  
  • Jin, L., Guzik, B. W., Bor, Y.-c., Rekosh, D., Hammarskjold, M.-L. (2003). Tap and NXT promote translation of unspliced mRNA. Genes Dev. 17: 3075-3086 [Abstract] [Full Text]  
  • Goff, A., Ehrlich, L. S., Cohen, S. N., Carter, C. A. (2003). Tsg101 Control of Human Immunodeficiency Virus Type 1 Gag Trafficking and Release. J. Virol. 77: 9173-9182 [Abstract] [Full Text]  
  • Javanbakht, H., Halwani, R., Cen, S., Saadatmand, J., Musier-Forsyth, K., Gottlinger, H., Kleiman, L. (2003). The Interaction between HIV-1 Gag and Human Lysyl-tRNA Synthetase during Viral Assembly. J. Biol. Chem. 278: 27644-27651 [Abstract] [Full Text]  
  • Halwani, R., Khorchid, A., Cen, S., Kleiman, L. (2003). Rapid Localization of Gag/GagPol Complexes to Detergent-Resistant Membrane during the Assembly of Human Immunodeficiency Virus Type 1. J. Virol. 77: 3973-3984 [Abstract] [Full Text]  
  • Coyle, J. H., Guzik, B. W., Bor, Y.-C., Jin, L., Eisner-Smerage, L., Taylor, S. J., Rekosh, D., Hammarskjold, M.-L. (2003). Sam68 Enhances the Cytoplasmic Utilization of Intron-Containing RNA and Is Functionally Regulated by the Nuclear Kinase Sik/BRK. Mol. Cell. Biol. 23: 92-103 [Abstract] [Full Text]  
  • Khorchid, A., Halwani, R., Wainberg, M. A., Kleiman, L. (2002). Role of RNA in Facilitating Gag/Gag-Pol Interaction. J. Virol. 76: 4131-4137 [Abstract] [Full Text]  
  • Dangel, A. W., Hull, S., Roberts, T. M., Boris-Lawrie, K. (2002). Nuclear Interactions Are Necessary for Translational Enhancement by Spleen Necrosis Virus RU5. J. Virol. 76: 3292-3300 [Abstract] [Full Text]  
  • West, J. T., Weldon, S. K., Wyss, S., Lin, X., Yu, Q., Thali, M., Hunter, E. (2002). Mutation of the Dominant Endocytosis Motif in Human Immunodeficiency Virus Type 1 gp41 Can Complement Matrix Mutations without Increasing Env Incorporation. J. Virol. 76: 3338-3349 [Abstract] [Full Text]  
  • West, J. T., Johnston, P. B., Dubay, S. R., Hunter, E. (2001). Mutations within the Putative Membrane-Spanning Domain of the Simian Immunodeficiency Virus Transmembrane Glycoprotein Define the Minimal Requirements for Fusion, Incorporation, and Infectivity. J. Virol. 75: 9601-9612 [Abstract] [Full Text]  
  • Cen, S., Khorchid, A., Javanbakht, H., Gabor, J., Stello, T., Shiba, K., Musier-Forsyth, K., Kleiman, L. (2001). Incorporation of Lysyl-tRNA Synthetase into Human Immunodeficiency Virus Type 1. J. Virol. 75: 5043-5048 [Abstract] [Full Text]  
  • Guzik, B. W., Levesque, L., Prasad, S., Bor, Y.-C., Black, B. E., Paschal, B. M., Rekosh, D., Hammarskjöld, M.-L. (2001). NXT1 (p15) Is a Crucial Cellular Cofactor in TAP-Dependent Export of Intron-Containing RNA in Mammalian Cells. Mol. Cell. Biol. 21: 2545-2554 [Abstract] [Full Text]  
  • Shehu-Xhilaga, M., Crowe, S. M., Mak, J. (2001). Maintenance of the Gag/Gag-Pol Ratio Is Important for Human Immunodeficiency Virus Type 1 RNA Dimerization and Viral Infectivity. J. Virol. 75: 1834-1841 [Abstract] [Full Text]  
  • Mansky, L. M., Bernard, L. C. (2000). 3'-Azido-3'-Deoxythymidine (AZT) and AZT-Resistant Reverse Transcriptase Can Increase the In Vivo Mutation Rate of Human Immunodeficiency Virus Type 1. J. Virol. 74: 9532-9539 [Abstract] [Full Text]  
  • Olivetta, E., Pugliese, K., Bona, R., D'Aloja, P., Ferrantelli, F., Santarcangelo, A. C., Mattia, G., Verani, P., Federico, M. (2000). cis Expression of the F12 Human Immunodeficiency Virus (HIV) Nef Allele Transforms the Highly Productive NL4-3 HIV Type 1 to a Replication-Defective Strain: Involvement of both Env gp41 and CD4 Intracytoplasmic Tails. J. Virol. 74: 483-492 [Abstract] [Full Text]  
  • Buchschacher, G. L. Jr., Yu, L., Murai, F., Friedmann, T., Miyanohara, A. (1999). Association of Murine Leukemia Virus Pol with Virions, Independent of Gag-Pol Expression. J. Virol. 73: 9632-9637 [Abstract] [Full Text]  
  • Hamm, T. E., Rekosh, D., Hammarskjöld, M.-L. (1999). Selection and Characterization of Human Immunodeficiency Virus Type 1 Mutants That Are Resistant to Inhibition by the Transdominant Negative RevM10 Protein. J. Virol. 73: 5741-5747 [Abstract] [Full Text]  
  • Butsch, M., Hull, S., Wang, Y., Roberts, T. M., Boris-Lawrie, K. (1999). The 5' RNA Terminus of Spleen Necrosis Virus Contains a Novel Posttranscriptional Control Element That Facilitates Human Immunodeficiency Virus Rev/RRE-Independent Gag Production. J. Virol. 73: 4847-4855 [Abstract] [Full Text]  
  • Helga-Maria, C., Hammarskjöld, M.-L., Rekosh, D. (1999). An Intact TAR Element and Cytoplasmic Localization Are Necessary for Efficient Packaging of Human Immunodeficiency Virus Type 1 Genomic RNA. J. Virol. 73: 4127-4135 [Abstract] [Full Text]  
  • Cen, S., Huang, Y., Khorchid, A., Darlix, J.-L., Wainberg, M. A., Kleiman, L. (1999). The Role of Pr55gag in the Annealing of tRNA3Lys to Human Immunodeficiency Virus Type 1 Genomic RNA. J. Virol. 73: 4485-4488 [Abstract] [Full Text]  
  • Gross, I., Hohenberg, H., Huckhagel, C., Krausslich, H.-G. (1998). N-Terminal Extension of Human Immunodeficiency Virus Capsid Protein Converts the In Vitro Assembly Phenotype from Tubular to Spherical Particles. J. Virol. 72: 4798-4810 [Abstract] [Full Text]  
  • Huang, Y., Khorchid, A., Gabor, J., Wang, J., Li, X., Darlix, J.-L., Wainberg, M. A., Kleiman, L. (1998). The Role of Nucleocapsid and U5 Stem/A-Rich Loop Sequences in tRNA3Lys Genomic Placement and Initiation of Reverse Transcription in Human Immunodeficiency Virus Type 1. J. Virol. 72: 3907-3915 [Abstract] [Full Text]  
  • D'Aloja, P., Olivetta, E., Bona, R., Nappi, F., Pedacchia, D., Pugliese, K., Ferrari, G., Verani, P., Federico, M. (1998). gag, vif, and nef Genes Contribute to the Homologous Viral Interference Induced by a Nonproducer Human Immunodeficiency Virus Type 1 (HIV-1) Variant: Identification of Novel HIV-1-Inhibiting Viral Protein Mutants. J. Virol. 72: 4308-4319 [Abstract] [Full Text]  
  • Zhang, Y., Qian, H., Love, Z., Barklis, E. (1998). Analysis of the Assembly Function of the Human Immunodeficiency Virus Type 1 Gag Protein Nucleocapsid Domain. J. Virol. 72: 1782-1789 [Abstract] [Full Text]  
  • Sharma, S., Murai, F., Miyanohara, A., Friedmann, T. (1997). Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents. Proc. Natl. Acad. Sci. USA 94: 10803-10808 [Abstract] [Full Text]  
  • Morikawa, Y., Hinata, S., Tomoda, H., Goto, T., Nakai, M., Aizawa, C., Tanaka, H., mura, S. (1996). Complete Inhibition of Human Immunodeficiency Virus Gag Myristoylation Is Necessary for Inhibition of Particle Budding. J. Biol. Chem. 271: 2868-2873 [Abstract] [Full Text]  
  • Dundr, M, Leno, G., Hammarskjold, M., Rekosh, D, Helga-Maria, C, Olson, M. (1995). The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein. J. Cell Sci. 108: 2811-2823 [Abstract]  
  • Bergey, E. J., Cho, M.-I., Hammarskjold, M.-L., Rekosh, D., Levine, M. J., Blumberg, B. M., Epstein, L. G. (1993). Aggregation of Human Immunodeficiency Virus Type 1 by Human Salivary Secretions. CROBM 4: 467-474 [Abstract] [Full Text]  
  • VerPlank, L., Bouamr, F., LaGrassa, T. J., Agresta, B., Kikonyogo, A., Leis, J., Carter, C. A. (2001). Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55Gag. Proc. Natl. Acad. Sci. USA 98: 7724-7729 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»