This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by De Stasio, P R Articles by Taylor, M W Search for Related Content PubMed PubMed Citation Articles by De Stasio, P R Articles by Taylor, M W

Specific effect of interferon on the herpes simplex virus type 1 transactivation event.

Department of Biology, Indiana University, Bloomington 47405.

ABSTRACT

Human recombinant gamma interferon and to a lesser extent alpha interferon were shown to inhibit herpes simplex virus type 1 replication in the human cell lines WISH and HEp-2. Dot blot analysis of viral DNA synthesis and viral RNA transcription indicated that the inhibition occurred at an early step in infection. A study of the early events after herpes simplex virus type 1 infection indicated that adsorption, penetration, uncoating, and transport of viral DNA were not affected by interferon. Northern (RNA) blot analysis revealed that both immediate-early and delayed-early gene transcription was inhibited by interferon. Transactivation of the immediate-early responsive element linked to a reporter gene (CAT or tk) was specifically inhibited by both classes of interferon. Our data would indicate that either the transactivating protein VP16 or the complex formed between VP16 and a host protein(s) is attenuated by interferon.

This article has been cited by other articles:

• Harandi, A. M., Eriksson, K., Holmgren, J. (2002). A Protective Role of Locally Administered Immunostimulatory CpG Oligodeoxynucleotide in a Mouse Model of Genital Herpes Infection. J. Virol. 77: 953-962 [Abstract] [Full Text]  
• Carr, D. J. J., Noisakran, S. (2002). The Antiviral Efficacy of the Murine Alpha-1 Interferon Transgene against Ocular Herpes Simplex Virus Type 1 Requires the Presence of CD4+, {alpha}/{beta} T-Cell Receptor-Positive T Lymphocytes with the Capacity To Produce Gamma Interferon. J. Virol. 76: 9398-9406 [Abstract] [Full Text]  
• Harle, P., Cull, V., Agbaga, M.-P., Silverman, R., Williams, B. R. G., James, C., Carr, D. J. J. (2002). Differential Effect of Murine Alpha/Beta Interferon Transgenes on Antagonization of Herpes Simplex Virus Type 1 Replication. J. Virol. 76: 6558-6567 [Abstract] [Full Text]  
• Mossman, K. L., Smiley, J. R. (2002). Herpes Simplex Virus ICP0 and ICP34.5 Counteract Distinct Interferon-Induced Barriers to Virus Replication. J. Virol. 76: 1995-1998 [Abstract] [Full Text]  
• Harandi, A. M., Svennerholm, B., Holmgren, J., Eriksson, K. (2001). Differential roles of B cells and IFN-{{gamma}}-secreting CD4+ T cells in innate and adaptive immune control of genital herpes simplex virus type 2 infection in mice. J. Gen. Virol. 82: 845-853 [Abstract] [Full Text]  
• Mossman, K. L., Saffran, H. A., Smiley, J. R. (2000). Herpes Simplex Virus ICP0 Mutants Are Hypersensitive to Interferon. J. Virol. 74: 2052-2056 [Abstract] [Full Text]  
• Yang, L., Voytek, C. C., Margolis, T. P. (2000). Immunohistochemical Analysis of Primary Sensory Neurons Latently Infected with Herpes Simplex Virus Type 1. J. Virol. 74: 209-217 [Abstract] [Full Text]  
• Cantin, E., Tanamachi, B., Openshaw, H., Mann, J., Clarke, K. (1999). Gamma Interferon (IFN-gamma ) Receptor Null-Mutant Mice Are More Susceptible to Herpes Simplex Virus Type 1 Infection than IFN-gamma Ligand Null-Mutant Mice. J. Virol. 73: 5196-5200 [Abstract] [Full Text]