This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brake, D A Articles by Debouck, C Search for Related Content PubMed PubMed Citation Articles by Brake, D A Articles by Debouck, C

 Previous Article  |  Next Article 

J Virol. 1990 February; 64(2): 962-965

Characterization of murine monoclonal antibodies to the tat protein from human immunodeficiency virus type 1.

Department of Molecular Genetics, Smith Kline and French Laboratories, King of Prussia, Pennsylvania 19406-0939.

ABSTRACT

A panel of murine monoclonal antibodies (MAbs) to the human immunodeficiency virus type 1 trans-activator tat protein were characterized. The anti-tat MAbs were mapped to the different domains of the tat protein by Western blot (immunoblot) and Pepscan analyses. One-half of the MAbs tested mapped to the amino-terminal proline-rich region, and one-third of the MAbs tested mapped to the lysine-arginine-rich region of tat. The individual MAbs were tested for inhibition of tat-mediated trans activation, using a cell-based in vitro assay system. MAbs which mapped to the amino-terminal region of the tat protein demonstrated the highest degree of inhibition, whereas MAbs reactive to other portions of the molecule exhibited a less pronounced effect on tat function.

This article has been cited by other articles:

• Mascarell, L., Fayolle, C., Bauche, C., Ladant, D., Leclerc, C. (2005). Induction of Neutralizing Antibodies and Th1-Polarized and CD4-Independent CD8+ T-Cell Responses following Delivery of Human Immunodeficiency Virus Type 1 Tat Protein by Recombinant Adenylate Cyclase of Bordetella pertussis. J. Virol. 79: 9872-9884 [Abstract] [Full Text]  
• Ruckwardt, T. J., Tikhonov, I., Berg, S., Hatfield, G. S., Chandra, A., Chandra, P., Gilliam, B., Redfield, R. R., Gallo, R. C., Pauza, C. D. (2004). Sequence Variation within the Dominant Amino Terminus Epitope Affects Antibody Binding and Neutralization of Human Immunodeficiency Virus Type 1 Tat Protein. J. Virol. 78: 13190-13196 [Abstract] [Full Text]  
• Moreau, E., Belliard, G., Partidos, C. D., Pradezinsky, F., Le Buanec, H., Muller, S., Desgranges, C. (2004). Important B-cell epitopes for neutralization of human immunodeficiency virus type 1 Tat in serum samples of humans and different animal species immunized with Tat protein or peptides. J. Gen. Virol. 85: 2893-2901 [Abstract] [Full Text]  
• Ramakrishna, L., Anand, K. K., Mohankumar, K. M., Ranga, U. (2004). Codon Optimization of the Tat Antigen of Human Immunodeficiency Virus Type 1 Generates Strong Immune Responses in Mice following Genetic Immunization. J. Virol. 78: 9174-9189 [Abstract] [Full Text]  
• Moreau, E., Hoebeke, J., Zagury, D., Muller, S., Desgranges, C. (2004). Generation and Characterization of Neutralizing Human Monoclonal Antibodies against Human Immunodeficiency Virus Type 1 Tat Antigen. J. Virol. 78: 3792-3796 [Abstract] [Full Text]  
• Tikhonov, I., Ruckwardt, T. J., Hatfield, G. S., Pauza, C. D. (2003). Tat-Neutralizing Antibodies in Vaccinated Macaques. J. Virol. 77: 3157-3166 [Abstract] [Full Text]