This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Innes, C L Articles by Boone, L R Search for Related Content PubMed PubMed Citation Articles by Innes, C L Articles by Boone, L R

 Previous Article  |  Next Article 

J Virol. 1990 February; 64(2): 957-961

Cationic liposomes (Lipofectin) mediate retroviral infection in the absence of specific receptors.

Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.

ABSTRACT

We have used cationic liposomes (Lipofectin) to facilitate retrovirus infection of cells lacking the homologous viral receptor. Ecotropic murine leukemia virus and packaged retroviral vectors were shown to infect mink cells, and amphotropic packaged retroviral vectors were shown to infect hamster cells in the presence of Lipofectin but not in the presence of Polybrene. Lipofectin-mediated infection of cells lacking the homologous receptor results in a titer approximately 0.1% of the titer in cells with the homologous receptor, using the standard Polybrene protocol. The use of Lipofectin may provide a simple means to experimentally infect a wide variety of cells with viruses not normally infectious for the species, tissue, or cell type of interest.

This article has been cited by other articles:

• Hill, C. L., Bieniasz, P. D., McClure, M. O. (1999). Properties of human foamy virus relevant to its development as a vector for gene therapy. J. Gen. Virol. 80: 2003-2009 [Abstract] [Full Text]  
• Porter, C. D., Lukacs, K. V., Box, G., Takeuchi, Y., Collins, M. K. L. (1998). Cationic Liposomes Enhance the Rate of Transduction by a Recombinant Retroviral Vector In Vitro and In Vivo. J. Virol. 72: 4832-4840 [Abstract] [Full Text]  
• Havenga, M., Hoogerbrugge, P., Valerio, D., van Es, H. H.G. (1997). Retroviral Stem Cell Gene Therapy. Stem Cells 15: 162-179 [Abstract] [Full Text]