This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cochrane, A W
Right arrow Articles by Rosen, C A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cochrane, A W
Right arrow Articles by Rosen, C A

 Previous Article  |  Next Article 

J Virol. 1990 February; 64(2): 881-885

Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to function.

A W Cochrane, A Perkins and C A Rosen

Department of Molecular Oncology, Roche Research Center, Nutley, New Jersey 07110.

ABSTRACT

The human immunodeficiency virus rev gene product regulates the expression of viral structural genes. It was recently shown that Rev regulates the export of viral structural mRNAs from the nucleus to the cytoplasm. Analysis of Rev subcellular localization reveals marked accumulation in the nucleolus, suggesting a role for the nucleolus in this export process. We report here the identification of amino acid residues critical to the nucleolar localization of Rev. Consistent with this finding, a Rev/beta-galactosidase fusion protein, harboring this region of Rev, localized entirely within the nucleolus. Of most significance, mutations that eliminated nucleolar localization markedly diminished Rev function, even though accumulation in the nucleoplasm was retained. These findings support a model whereby Rev-induced export of human immunodeficiency virus structural mRNAs from the nucleus to the cytoplasm is likely to involve nucleolar events.

J Virol. 1990 February; 64(2): 881-885




This article has been cited by other articles:

  • Gomez Corredor, A., Archambault, D. (2009). The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Lentiviral Bipartite Nuclear Localization Signal Harboring an Atypical Spacer Sequence. J. Virol. 83: 12842-12853 [Abstract] [Full Text]  
  • Hofacre, A., Nitta, T., Fan, H. (2009). Jaagsiekte Sheep Retrovirus Encodes a Regulatory Factor, Rej, Required for Synthesis of Gag Protein. J. Virol. 83: 12483-12498 [Abstract] [Full Text]  
  • Hutten, S., Walde, S., Spillner, C., Hauber, J., Kehlenbach, R. H. (2009). The nuclear pore component Nup358 promotes transportin-dependent nuclear import. J. Cell Sci. 122: 1100-1110 [Abstract] [Full Text]  
  • Venter, P. A., Marshall, D., Schneemann, A. (2009). Dual Roles for an Arginine-Rich Motif in Specific Genome Recognition and Localization of Viral Coat Protein to RNA Replication Sites in Flock House Virus-Infected Cells. J. Virol. 83: 2872-2882 [Abstract] [Full Text]  
  • Boyne, J. R., Whitehouse, A. (2006). Nucleolar trafficking is essential for nuclear export of intronless herpesvirus mRNA. Proc. Natl. Acad. Sci. USA 103: 15190-15195 [Abstract] [Full Text]  
  • Arnold, M., Nath, A., Hauber, J., Kehlenbach, R. H. (2006). Multiple Importins Function as Nuclear Transport Receptors for the Rev Protein of Human Immunodeficiency Virus Type 1. J. Biol. Chem. 281: 20883-20890 [Abstract] [Full Text]  
  • Choi, E.-A, Hope, T. J. (2005). Mutational Analysis of Bovine Leukemia Virus Rex: Identification of a Dominant-Negative Inhibitor. J. Virol. 79: 7172-7181 [Abstract] [Full Text]  
  • Daelemans, D., Costes, S. V., Cho, E. H., Erwin-Cohen, R. A., Lockett, S., Pavlakis, G. N. (2004). In Vivo HIV-1 Rev Multimerization in the Nucleolus and Cytoplasm Identified by Fluorescence Resonance Energy Transfer. J. Biol. Chem. 279: 50167-50175 [Abstract] [Full Text]  
  • Kong, K. Y., Kedes, L. (2004). Cytoplasmic Nuclear Transfer of the Actin-capping Protein Tropomodulin. J. Biol. Chem. 279: 30856-30864 [Abstract] [Full Text]  
  • Szebeni, A., Hingorani, K., Negi, S., Olson, M. O. J. (2003). Role of Protein Kinase CK2 Phosphorylation in the Molecular Chaperone Activity of Nucleolar Protein B23. J. Biol. Chem. 278: 9107-9115 [Abstract] [Full Text]  
  • Marques, S. M. P., Veyrune, J.-L., Shukla, R. R., Kumar, A. (2003). Restriction of Human Immunodeficiency Virus Type 1 Rev Function in Murine A9 Cells Involves the Rev C-Terminal Domain. J. Virol. 77: 3084-3090 [Abstract] [Full Text]  
  • Hakata, Y., Yamada, M., Mabuchi, N., Shida, H. (2002). The Carboxy-Terminal Region of the Human Immunodeficiency Virus Type 1 Protein Rev Has Multiple Roles in Mediating CRM1-Related Rev Functions. J. Virol. 76: 8079-8089 [Abstract] [Full Text]  
  • Li, J., Liu, Y., Kim, B. O., He, J. J. (2002). Direct Participation of Sam68, the 68-Kilodalton Src-Associated Protein in Mitosis, in the CRM1-Mediated Rev Nuclear Export Pathway. J. Virol. 76: 8374-8382 [Abstract] [Full Text]  
  • Koshizuka, T., Goshima, F., Takakuwa, H., Nozawa, N., Daikoku, T., Koiwai, O., Nishiyama, Y. (2002). Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2. J. Virol. 76: 6718-6728 [Abstract] [Full Text]  
  • Didichenko, S. A., Thelen, M. (2001). Phosphatidylinositol 3-Kinase C2alpha Contains a Nuclear Localization Sequence and Associates with Nuclear Speckles. J. Biol. Chem. 276: 48135-48142 [Abstract] [Full Text]  
  • Soros, V. B., Carvajal, H. V., Richard, S., Cochrane, A. W. (2001). Inhibition of Human Immunodeficiency Virus Type 1 Rev Function by a Dominant-Negative Mutant of Sam68 through Sequestration of Unspliced RNA at Perinuclear Bundles. J. Virol. 75: 8203-8215 [Abstract] [Full Text]  
  • Nam, Y.-S., Petrovic, A., Jeong, K.-S., Venkatesan, S. (2001). Exchange of the Basic Domain of Human Immunodeficiency Virus Type 1 Rev for a Polyarginine Stretch Expands the RNA Binding Specificity, and a Minimal Arginine Cluster Is Required for Optimal RRE RNA Binding Affinity, Nuclear Accumulation, and trans-Activation. J. Virol. 75: 2957-2971 [Abstract] [Full Text]  
  • Neumann, M, Afonina, E, Ceccherini-Silberstein, F, Schlicht, S, Erfle, V, Pavlakis, G., Brack-Werner, R (2001). Nucleocytoplasmic transport in human astrocytes: decreased nuclear uptake of the HIV Rev shuttle protein. J. Cell Sci. 114: 1717-1729 [Abstract]  
  • Rosorius, O., Fries, B., Stauber, R. H., Hirschmann, N., Bevec, D., Hauber, J. (2000). Human Ribosomal Protein L5 Contains Defined Nuclear Localization and Export Signals. J. Biol. Chem. 275: 12061-12068 [Abstract] [Full Text]  
  • Weber, J. D., Kuo, M.-L., Bothner, B., DiGiammarino, E. L., Kriwacki, R. W., Roussel, M. F., Sherr, C. J. (2000). Cooperative Signals Governing ARF-Mdm2 Interaction and Nucleolar Localization of the Complex. Mol. Cell. Biol. 20: 2517-2528 [Abstract] [Full Text]  
  • Clau{beta}en, M., Rudt, F., Pieler, T. (1999). Functional Modules in Ribosomal Protein L5 for Ribonucleoprotein Complex Formation and Nucleocytoplasmic Transport. J. Biol. Chem. 274: 33951-33958 [Abstract] [Full Text]  
  • Szilvay, A. M., Bøe, S.-O., Kalland, K.-H. (1999). Co-expression of a trans-dominant negative mutant of the human immunodeficiency virus type 1 (HIV-1) Rev protein affects the Rev-dependent splicing pattern and expression of HIV-1 RNAs. J. Gen. Virol. 80: 1965-1974 [Abstract] [Full Text]  
  • Lam, M. H. C., House, C. M., Tiganis, T., Mitchelhill, K. I., Sarcevic, B., Cures, A., Ramsay, R., Kemp, B. E., Martin, T. J., Gillespie, M. T. (1999). Phosphorylation at the Cyclin-dependent Kinases Site (Thr85) of Parathyroid Hormone-related Protein Negatively Regulates Its Nuclear Localization. J. Biol. Chem. 274: 18559-18566 [Abstract] [Full Text]  
  • Van Ryk, D. I., Venkatesan, S. (1999). Real-time Kinetics of HIV-1 Rev-Rev Response Element Interactions. DEFINITION OF MINIMAL BINDING SITES ON RNA AND PROTEIN AND STOICHIOMETRIC ANALYSIS. J. Biol. Chem. 274: 17452-17463 [Abstract] [Full Text]  
  • Truant, R., Cullen, B. R. (1999). The Arginine-Rich Domains Present in Human Immunodeficiency Virus Type 1 Tat and Rev Function as Direct Importin beta -Dependent Nuclear Localization Signals. Mol. Cell. Biol. 19: 1210-1217 [Abstract] [Full Text]  
  • Askjaer, P., Jensen, T. H., Nilsson, J., Englmeier, L., Kjems, J. (1998). The Specificity of the CRM1-Rev Nuclear Export Signal Interaction Is Mediated by RanGTP. J. Biol. Chem. 273: 33414-33422 [Abstract] [Full Text]  
  • Hakata, Y., Umemoto, T., Matsushita, S., Shida, H. (1998). Involvement of Human CRM1 (Exportin 1) in the Export and Multimerization of the Rex Protein of Human T-Cell Leukemia Virus Type 1. J. Virol. 72: 6602-6607 [Abstract] [Full Text]  
  • Thomas, S. L., Oft, M., Jaksche, H., Casari, G., Heger, P., Dobrovnik, M., Bevec, D., Hauber, J. (1998). Functional Analysis of the Human Immunodeficiency Virus Type 1 Rev Protein Oligomerization Interface. J. Virol. 72: 2935-2944 [Abstract] [Full Text]  
  • Schatz, O., Oft, M., Dascher, C., Schebesta, M., Rosorius, O., Jaksche, H., Dobrovnik, M., Bevec, D., Hauber, J. (1998). Interaction of the HIV-1 Rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5. Proc. Natl. Acad. Sci. USA 95: 1607-1612 [Abstract] [Full Text]  
  • Efthymiadis, A., Briggs, L. J., Jans, D. A. (1998). The HIV-1 Tat Nuclear Localization Sequence Confers Novel Nuclear Import Properties. J. Biol. Chem. 273: 1623-1628 [Abstract] [Full Text]  
  • Cardoso, M. C., Joseph, C., Rahn, H.-P., Reusch, R., Nadal-Ginard, B., Leonhardt, H. (1997). Mapping and Use of a Sequence that Targets DNA Ligase I to Sites of DNA Replication In Vivo. JCB 139: 579-587 [Abstract] [Full Text]  
  • Zirwes, R. F., Schmidt-Zachmann, M. S., Franke, W. W. (1997). Identification of a small, very acidic constitutive nucleolar protein (NO29) as a member of the nucleoplasmin family. Proc. Natl. Acad. Sci. USA 94: 11387-11392 [Abstract] [Full Text]  
  • Dundr, M, Leno, G., Hammarskjold, M., Rekosh, D, Helga-Maria, C, Olson, M. (1995). The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein. J. Cell Sci. 108: 2811-2823 [Abstract]  
  • Ochs, R., Stein, T., Tan, E. (1994). Coiled bodies in the nucleolus of breast cancer cells. J. Cell Sci. 107: 385-399 [Abstract]  
  • Schmidt-Zachmann, M., Nigg, E. (1993). Protein localization to the nucleolus: a search for targeting domains in nucleolin. J. Cell Sci. 105: 799-806 [Abstract]  
  • Baserga, S J, Gilmore-Hebert, M, Yang, X W (1992). Distinct molecular signals for nuclear import of the nucleolar snRNA, U3.. Genes Dev. 6: 1120-1130 [Abstract]  
  • Olsen, H S, Cochrane, A W, Dillon, P J, Nalin, C M, Rosen, C A (1990). Interaction of the human immunodeficiency virus type 1 Rev protein with a structured region in env mRNA is dependent on multimer formation mediated through a basic stretch of amino acids.. Genes Dev. 4: 1357-1364 [Abstract]  
  • Goodwin, D. J., Whitehouse, A. (2001). A gamma -2 Herpesvirus Nucleocytoplasmic Shuttle Protein Interacts with Importin alpha 1 and alpha 5. J. Biol. Chem. 276: 19905-19912 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»