This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fuerstenberg, S
Right arrow Articles by Zenke, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fuerstenberg, S
Right arrow Articles by Zenke, M

 Previous Article  |  Next Article 

J Virol. 1990 December; 64(12): 5891-5902

Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector.

S Fuerstenberg, H Beug, M Introna, K Khazaie, A Muñoz, S Ness, K Nordström, J Sap, I Stanley and M Zenke

Department of Molecular Biology, Karolinska Institute, Stockholm, Sweden.

ABSTRACT

A retrovirus vector was constructed from the genome of avian erythroblastosis virus ES4. The v-erbA sequences of avian erythroblastosis virus were replaced by those coding for neomycin phosphotransferase, creating a gag-neo fusion protein which provides G418 resistance as a selectable marker. The v-erbB sequences following the splice acceptor were replaced by a cloning linker allowing insertion of foreign genes. The vector has been tested in conjunction with several helper viruses for the transmission of G418 resistance, titer, stability, transcription, and the transduction and expression of foreign genes in both chicken embryo fibroblasts and the QT6 quail cell line. The results show that the vector is capable of producing high titers of Neor virus from stably integrated proviruses. These proviruses express a balanced ratio of genome length to spliced transcripts which are efficiently translated into protein. Using the Escherichia coli beta-galactosidase gene cloned into the vector as a test construct, expression of enzyme activity could be detected in 90 to 95% of transfected target cells and in 80 to 85% of subsequently infected cells. In addition, a cDNA encoding the avian erythrocyte band 3 anion exchange protein has been expressed from the vector in both chicken embryo fibroblasts and QT6 cells and appears to function as an active, plasma membrane-based anion transporter. The ectopic expression of band 3 protein provides a visual marker for vector function in these cells.

J Virol. 1990 December; 64(12): 5891-5902




This article has been cited by other articles:

  • Crouch, D. H., Fisher, F., La Rocca, S. A., Goding, C. R., Gillespie, D. A. F. (2005). Viral mutations enhance the Max binding properties of the vMyc b-HLH-LZ domain. Nucleic Acids Res 33: 5235-5242 [Abstract] [Full Text]  
  • Segat, D., Comai, R., Di Marco, E., Strangio, A., Cancedda, R., Franzi, A. T., Tacchetti, C. (2002). Integrins alpha 6Abeta 1 and alpha 6Bbeta 1 Promote Different Stages of Chondrogenic Cell Differentiation. J. Biol. Chem. 277: 31612-31622 [Abstract] [Full Text]  
  • Carragher, N. O., Westhoff, M. A., Riley, D., Potter, D. A., Dutt, P., Elce, J. S., Greer, P. A., Frame, M. C. (2002). v-Src-Induced Modulation of the Calpain-Calpastatin Proteolytic System Regulates Transformation. Mol. Cell. Biol. 22: 257-269 [Abstract] [Full Text]  
  • Querfurth, E., Schuster, M., Kulessa, H., Crispino, J. D., Döderlein, G., Orkin, S. H., Graf, T., Nerlov, C. (2000). Antagonism between C/EBPbeta and FOG in eosinophil lineage commitment of multipotent hematopoietic progenitors. Genes Dev. 14: 2515-2525 [Abstract] [Full Text]  
  • Fincham, V. J., Brunton, V. G., Frame, M. C. (2000). The SH3 Domain Directs Acto-Myosin-Dependent Targeting of v-Src to Focal Adhesions via Phosphatidylinositol 3-Kinase. Mol. Cell. Biol. 20: 6518-6536 [Abstract] [Full Text]  
  • Clark, W., Black, E. J., MacLaren, A., Kruse, U., LaThangue, N., Vogt, P. K., Gillespie, D. A. F. (2000). v-Jun Overrides the Mitogen Dependence of S-Phase Entry by Deregulating Retinoblastoma Protein Phosphorylation and E2F-Pocket Protein Interactions as a Consequence of Enhanced Cyclin E-cdk2 Catalytic Activity. Mol. Cell. Biol. 20: 2529-2542 [Abstract] [Full Text]  
  • Madruga, J, Koritschoner, N., Diebold, S., Kurz, S., Zenke, M (1999). Polarised expression pattern of focal contact proteins in highly motile antigen presenting dendritic cells. J. Cell Sci. 112: 1685-1696 [Abstract]  
  • Fincham, V., Chudleigh, A, Frame, M. (1999). Regulation of p190 Rho-GAP by v-Src is linked to cytoskeletal disruption during transformation. J. Cell Sci. 112: 947-956 [Abstract]  
  • Bartunek, P., Zenke, M. (1998). Retinoid X Receptor and c-erbA/Thyroid Hormone Receptor Regulate Erythroid Cell Growth and Differentiation. Mol. Endocrinol. 12: 1269-1279 [Abstract] [Full Text]  
  • Briegel, K, Bartunek, P, Stengl, G, Lim, K., Beug, H, Engel, J., Zenke, M (1996). Regulation and function of transcription factor GATA-1 during red blood cell differentiation. Development 122: 3839-3850 [Abstract]  
  • Kulessa, H, Frampton, J, Graf, T (1995). GATA-1 reprograms avian myelomonocytic cell lines into eosinophils, thromboblasts, and erythroblasts.. Genes Dev. 9: 1250-1262 [Abstract]  
  • Briegel, K, Lim, K C, Plank, C, Beug, H, Engel, J D, Zenke, M (1993). Ectopic expression of a conditional GATA-2/estrogen receptor chimera arrests erythroid differentiation in a hormone-dependent manner.. Genes Dev. 7: 1097-1109 [Abstract]  
  • Disela, C, Glineur, C, Bugge, T, Sap, J, Stengl, G, Dodgson, J, Stunnenberg, H, Beug, H, Zenke, M (1991). v-erbA overexpression is required to extinguish c-erbA function in erythroid cell differentiation and regulation of the erbA target gene CAII.. Genes Dev. 5: 2033-2047 [Abstract]  
  • Metz, T, Graf, T (1991). v-myb and v-ets transform chicken erythroid cells and cooperate both in trans and in cis to induce distinct differentiation phenotypes.. Genes Dev. 5: 369-380 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»