This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balachandran, N
Right arrow Articles by Chang, C K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balachandran, N
Right arrow Articles by Chang, C K

 Previous Article  |  Next Article 

J Virol. 1989 June; 63(6): 2835-2840

Identification of proteins specific for human herpesvirus 6-infected human T cells.

N Balachandran, R E Amelse, W W Zhou and C K Chang

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.

ABSTRACT

Proteins specific for human herpesvirus 6 (HHV-6)-infected human T cells (HSB-2) were examined by using polyclonal rabbit antibodies and monoclonal antibodies against HHV-6-infected cells and human sera. More than 20 proteins and six glycoproteins specific for HHV-6-infected cells were identified from [35S]methionine- and [3H]glucosamine-labeled total-cell extracts. Polyclonal rabbit antibodies immunoprecipitated 33 [35S]methionine-labeled HHV-6-specific polypeptides with approximate molecular weights ranging from 180,000 to 31,000. In immunoprecipitation and Western immunoblot reactions, a patient's serum also recognized more than 30 HHV-6-specific proteins and seven glycoproteins. In contrast, sera from individuals with high-titered antibodies against other human herpesviruses reacted with fewer HHV-6-infected cell proteins, and only a 135,000-Mr polypeptide was prominent. Monoclonal antibodies to HHV-6-infected cells reacted with single and multiple polypeptides specific for virus-infected cells and immunoprecipitated three distinct sets of glycoproteins, which were designated gp105k and gp82k, gp116k, gp64k, and gp54k, and gp102k.

J Virol. 1989 June; 63(6): 2835-2840




This article has been cited by other articles:

  • Donati, D., Akhyani, N., Fogdell-Hahn, A., Cermelli, C., Cassiani-Ingoni, R., Vortmeyer, A., Heiss, J. D., Cogen, P., Gaillard, W. D., Sato, S., Theodore, W. H., Jacobson, S. (2003). Detection of human herpesvirus-6 in mesial temporal lobe epilepsy surgical brain resections. Neurology 61: 1405-1411 [Abstract] [Full Text]  
  • Santoro, F., Greenstone, H. L., Insinga, A., Liszewski, M. K., Atkinson, J. P., Lusso, P., Berger, E. A. (2003). Interaction of Glycoprotein H of Human Herpesvirus 6 with the Cellular Receptor CD46. J. Biol. Chem. 278: 25964-25969 [Abstract] [Full Text]  
  • Xu, Y., Linde, A., Dahl, H., Winberg, G. (2001). Definition of a Divergent Epitope That Allows Differential Detection of Early Protein p41 from Human Herpesvirus 6 Variants A and B. J. Clin. Microbiol. 39: 1449-1455 [Abstract] [Full Text]  
  • Flebbe-Rehwaldt, L. M., Wood, C., Chandran, B. (2000). Characterization of Transcripts Expressed from Human Herpesvirus 6A Strain GS Immediate-Early Region B U16-U17 Open Reading Frames. J. Virol. 74: 11040-11054 [Abstract] [Full Text]  
  • Enbom, M., Wang, F.-Z., Fredrikson, S., Martin, C., Dahl, H., Linde, A. (1999). Similar Humoral and Cellular Immunological Reactivities to Human Herpesvirus 6 in Patients with Multiple Sclerosis and Controls. CVI 6: 545-549 [Abstract] [Full Text]  
  • Gobbi, A., Stoddart, C. A., Malnati, M. S., Locatelli, G., Santoro, F., Abbey, N. W., Bare, C., Linquist-Stepps, V., Moreno, M. B., Herndier, B. G., Lusso, P., McCune, J. M. (1999). Human Herpesvirus 6 (HHV-6) Causes Severe Thymocyte Depletion in SCID-hu Thy/Liv Mice. JEM 189: 1953-1960 [Abstract] [Full Text]  
  • Wallace, H. L. II, Natelson, B., Gause, W., Hay, J. (1999). Human Herpesviruses in Chronic Fatigue Syndrome. CVI 6: 216-223 [Abstract] [Full Text]  
  • Cardinali, G., Gentile, M., Cirone, M., Zompetta, C., Frati, L., Faggioni, A., Torrisi, M. R. (1998). Viral Glycoproteins Accumulate in Newly Formed Annulate Lamellae following Infection of Lymphoid Cells by Human Herpesvirus 6. J. Virol. 72: 9738-9746 [Abstract] [Full Text]  
  • Rotola, A., Ravaioli, T., Gonelli, A., Dewhurst, S., Cassai, E., Di Luca, D. (1998). U94 of human herpesvirus 6 is expressed in latently infected peripheral blood mononuclear cells and blocks viral gene expression in transformed lymphocytes in culture. Proc. Natl. Acad. Sci. USA 95: 13911-13916 [Abstract] [Full Text]  
  • Cocchi, F., DeVico, A. L., Garzino-Demo, A., Arya, S. K., Gallo, R. C., Lusso, P. (1995). Identification of RANTES, MIP-1alpha, and MIP-1beta as the Major HIV-Suppressive Factors Produced by CD8+ T Cells. Science 270: 1811-1815 [Abstract]  
  • Buchwald, D., Cheney, P. R., Peterson, D. L., Henry, B., Wormsley, S. B., Geiger, A., Ablashi, D. V., Salahuddin, S. Z., Saxinger, C., Biddle, R., Kikinis, R., Jolesz, F. A., Folks, T., Balachandran, N., Peter, J. B., Gallo, R. C., Komaroff, A. L. (1992). A Chronic Illness Characterized by Fatigue, Neurologic and Immunologic Disorders, and Active Human Herpesvirus Type 6 Infection. ANN INTERN MED 116: 103-113 [Abstract]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»