This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Raines, M A Articles by Kung, H J Search for Related Content PubMed PubMed Citation Articles by Raines, M A Articles by Kung, H J

 Previous Article  |  Next Article 

J Virol. 1988 July; 62(7): 2437-2443

Mechanism of c-erbB transduction: newly released transducing viruses retain poly(A) tracts of erbB transcripts and encode C-terminally intact erbB proteins.

M A Raines, N J Maihle, C Moscovici, L Crittenden and H J Kung

Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.

ABSTRACT

We have previously shown that avian leukosis virus (ALV) induces erythroblastosis by insertional activation of the c-erbB gene. In 25% of the ALV-induced leukemic samples we have analyzed, acute retroviruses that have captured the activated erbB oncogene were released. The unusually high frequency at which erbB transduction occurs makes this an ideal system for studying the mechanism of oncogene transduction. In addition, these leukemic samples provide a rich source for the isolation of novel erbB-transducing viruses. We report here our characterization of several new erbB-transducing proviruses. The 5' recombination points of all these viruses mapped to the same intron in which proviral insertions cluster, supporting the hypothesis that transduction begins with proviral insertion near the oncogene. The 3' recombination points usually occurred within the 3' untranslated region downstream from the termination codon of the c-erbB gene. Three of the erbB-containing proviruses were molecularly cloned and analyzed in detail. Two of them were capable of releasing acute viruses, and interestingly, both retained poly(A) tracts of erbB messages in their genomes. A stretch of six adenosine residues in the ALV env gene appeared to mediate the 3' recombination events required for the generation of these viruses. These data provide further insight into the mechanism by which oncogenes are transduced into retroviral genomes.

---

J Virol. 1988 July; 62(7): 2437-2443

This article has been cited by other articles:

• An, W., Telesnitsky, A. (2004). Human Immunodeficiency Virus Type 1 Transductive Recombination Can Occur Frequently and in Proportion to Polyadenylation Signal Readthrough. J. Virol. 78: 3419-3428 [Abstract] [Full Text]  
• Chesters, P. M., Howes, K., Petherbridge, L., Evans, S., Payne, L. N., Venugopal, K. (2002). The viral envelope is a major determinant for the induction of lymphoid and myeloid tumours by avian leukosis virus subgroups A and J, respectively. J. Gen. Virol. 83: 2553-2561 [Abstract] [Full Text]  
• Sun, G., O'Neil, P. K., Yu, H., Ron, Y., Preston, B. D., Dougherty, J. P. (2001). Transduction of Cellular Sequence by a Human Immunodeficiency Virus Type 1-Derived Vector. J. Virol. 75: 11902-11906 [Abstract] [Full Text]  
• Zhang, J., Tang, L.-Y., Li, T., Ma, Y., Sapp, C. M. (2000). Most Retroviral Recombinations Occur during Minus-Strand DNA Synthesis. J. Virol. 74: 2313-2322 [Abstract] [Full Text]  
• Boerner, J., McManus, M., Martin, G., Maihle, N. (2000). Ras-independent oncogenic transformation by an EGF-receptor mutant. J. Cell Sci. 113: 935-942 [Abstract]  
• Swain, A, Coffin, J. (1992). Mechanism of transduction by retroviruses. Science 255: 841-845 [Abstract]