This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brown, N A Articles by Garcia, C R Search for Related Content PubMed PubMed Citation Articles by Brown, N A Articles by Garcia, C R

 Previous Article  |  Next Article 

J Virol. 1988 March; 62(3): 962-969

B-cell lymphoproliferation and lymphomagenesis are associated with clonotypic intracellular terminal regions of the Epstein-Barr virus.

Department of Pediatrics, University of California, Los Angeles School of Medicine 90024.

ABSTRACT

We analyzed 17 B-cell lineages cloned from two patients with infectious mononucleosis and found that different B-cell lineages exhibited notable variation in the length of the fused Epstein-Barr virus (EBV) terminal region on intracellular EBV episomes. EBV termini in different B-cell clones from the same person differed by as many as 15 to 20 reiterations of the ca. 500-base-pair terminal repeat sequence. In contrast, analysis of seven B-cell lineages cloned from a patient with a fatal, oligoclonal lymphoma revealed that three of the cell clones had the same-sized EBV terminal region. These three clones had previously been shown, by immunoglobulin gene analysis, to be metastatic daughter cells descended from a common progenitor. Similarity of the EBV terminal regions in the three daughter clones suggested that EBV infected the progenitor cell before proliferation and metastasis. Individual, EBV-infected cells from a single individual showed sufficient heterogeneity in their EBV termini to allow use of terminal fragment size as a clonal marker in studies addressing the contribution of EBV to the clonal pathogenesis of tumors with which this virus has been associated.

This article has been cited by other articles:

• Moody, C. A., Scott, R. S., Amirghahari, N., Nathan, C.-A., Young, L. S., Dawson, C. W., Sixbey, J. W. (2005). Modulation of the Cell Growth Regulator mTOR by Epstein-Barr Virus-Encoded LMP2A. J. Virol. 79: 5499-5506 [Abstract] [Full Text]  
• Lit, L. C.W., Chan, K.C. A., Leung, S.-F., Lei, K. I.K., Chan, L. Y.S., Chow, K. C.K., Chan, A. T.C., Lo, Y.M. D. (2004). Distribution of Cell-Free and Cell-Associated Epstein-Barr Virus (EBV) DNA in the Blood of Patients with Nasopharyngeal Carcinoma and EBV-Associated Lymphoma. Clin. Chem. 50: 1842-1845 [Full Text]  
• Moody, C. A., Scott, R. S., Su, T., Sixbey, J. W. (2003). Length of Epstein-Barr Virus Termini as a Determinant of Epithelial Cell Clonal Emergence. J. Virol. 77: 8555-8561 [Abstract] [Full Text]