This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hauber, J
Right arrow Articles by Cullen, B R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hauber, J
Right arrow Articles by Cullen, B R

 Previous Article  |  Next Article 

J Virol. 1988 December; 62(12): 4801-4804

Phosphorylation of the rev gene product of human immunodeficiency virus type 1.

J Hauber, M Bouvier, M H Malim and B R Cullen

Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.

ABSTRACT

Replication of human immunodeficiency virus type 1 requires the functional expression in trans of the virally encoded rev gene product (previously called art/trs). Here we demonstrate that this protein can be metabolically labeled with 32Pi. The phosphate receptor in the rev protein is shown to be exclusively serine. Treatment of rev-expressing cells with phorbol ester, a specific activator of protein kinase C, led to significant but transient enhancement of the level of rev phosphorylation. These results indicate that the rev protein is posttranslationally modified in vivo and suggest that the level of this modification is subject to modulation by extracellular stimuli.

J Virol. 1988 December; 62(12): 4801-4804




This article has been cited by other articles:

  • Yueh, A., Goff, S. P. (2003). Phosphorylated Serine Residues and an Arginine-Rich Domain of the Moloney Murine Leukemia Virus p12 Protein Are Required for Early Events of Viral Infection. J. Virol. 77: 1820-1829 [Abstract] [Full Text]  
  • Hakata, Y., Yamada, M., Mabuchi, N., Shida, H. (2002). The Carboxy-Terminal Region of the Human Immunodeficiency Virus Type 1 Protein Rev Has Multiple Roles in Mediating CRM1-Related Rev Functions. J. Virol. 76: 8079-8089 [Abstract] [Full Text]  
  • Muller, B., Patschinsky, T., Krausslich, H.-G. (2002). The Late-Domain-Containing Protein p6 Is the Predominant Phosphoprotein of Human Immunodeficiency Virus Type 1 Particles. J. Virol. 76: 1015-1024 [Abstract] [Full Text]  
  • Barber, S. A., Bruett, L., Douglass, B. R., Herbst, D. S., Zink, M. C., Clements, J. E. (2002). Visna Virus-Induced Activation of MAPK Is Required for Virus Replication and Correlates with Virus-Induced Neuropathology. J. Virol. 76: 817-828 [Abstract] [Full Text]  
  • Neumann, M, Afonina, E, Ceccherini-Silberstein, F, Schlicht, S, Erfle, V, Pavlakis, G., Brack-Werner, R (2001). Nucleocytoplasmic transport in human astrocytes: decreased nuclear uptake of the HIV Rev shuttle protein. J. Cell Sci. 114: 1717-1729 [Abstract]  
  • Cartier, C., Sivard, P., Tranchat, C., Decimo, D., Desgranges, C., Boyer, V. (1999). Identification of Three Major Phosphorylation Sites within HIV-1 Capsid. ROLE OF PHOSPHORYLATION DURING THE EARLY STEPS OF INFECTION. J. Biol. Chem. 274: 19434-19440 [Abstract] [Full Text]  
  • Yang, X., Gabuzda, D. (1999). Regulation of Human Immunodeficiency Virus Type 1 Infectivity by the ERK Mitogen-Activated Protein Kinase Signaling Pathway. J. Virol. 73: 3460-3466 [Abstract] [Full Text]  
  • Yang, X., Gabuzda, D. (1998). Mitogen-activated Protein Kinase Phosphorylates and Regulates the HIV-1 Vif Protein. J. Biol. Chem. 273: 29879-29887 [Abstract] [Full Text]  
  • Thomas, S. L., Oft, M., Jaksche, H., Casari, G., Heger, P., Dobrovnik, M., Bevec, D., Hauber, J. (1998). Functional Analysis of the Human Immunodeficiency Virus Type 1 Rev Protein Oligomerization Interface. J. Virol. 72: 2935-2944 [Abstract] [Full Text]  
  • Brand, S. R., Kobayashi, R., Mathews, M. B. (1997). The Tat Protein of Human Immunodeficiency Virus Type 1Is a Substrate and Inhibitor of the Interferon-induced, Virally Activated Protein Kinase, PKR. J. Biol. Chem. 272: 8388-8395 [Abstract] [Full Text]  
  • Yang, X., Goncalves, J., Gabuzda, D. (1996). Phosphorylation of Vif and Its Role in HIV-1 Replication. J. Biol. Chem. 271: 10121-10129 [Abstract] [Full Text]  
  • Dundr, M, Leno, G., Lewis, N, Rekosh, D, Hammarskjoid, M., Olson, M. (1996). Location of the HIV-1 Rev protein during mitosis: inactivation of the nuclear export signal alters the pathway for postmitotic reentry into nucleoli. J. Cell Sci. 109: 2239-2251 [Abstract]  
  • Olsen, H S, Cochrane, A W, Dillon, P J, Nalin, C M, Rosen, C A (1990). Interaction of the human immunodeficiency virus type 1 Rev protein with a structured region in env mRNA is dependent on multimer formation mediated through a basic stretch of amino acids.. Genes Dev. 4: 1357-1364 [Abstract]  
  • Hanly, S M, Rimsky, L T, Malim, M H, Kim, J H, Hauber, J, Duc Dodon, M, Le, S Y, Maizel, J V, Cullen, B R, Greene, W C (1989). Comparative analysis of the HTLV-I Rex and HIV-1 Rev trans-regulatory proteins and their RNA response elements.. Genes Dev. 3: 1534-1544 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»