This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jeang, K T
Right arrow Articles by Khoury, G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jeang, K T
Right arrow Articles by Khoury, G

 Previous Article  |  Next Article 

J Virol. 1988 December; 62(12): 4499-4509

Characterization of cellular factors that interact with the human T-cell leukemia virus type I p40x-responsive 21-base-pair sequence.

K T Jeang, I Boros, J Brady, M Radonovich and G Khoury

Laboratory of Molecular Virology, National Cancer Institute, Bethesda, Maryland 20892.

ABSTRACT

Transcriptional activation of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) by viral protein p40x requires a 21-base-pair (bp) sequence which is repeated three times within the LTR. This sequence contains a core octanucleotide (TGACGTCT) which has been attributed to be a cyclic-AMP (cAMP)-responsive element. We demonstrate here that the HTLV-I LTR can be specifically stimulated by cAMP regulators and have identified four proteins in HeLa cells that bind to the HTLV-I 21-bp sequence. We correlated the in vitro binding and transcriptional activity of one of these cellular factors (Mr, 180,000) to the trans-activation of the HTLV-I LTR by p40x. Point mutations were generated within the cAMP octanucleotide of the HTLV-I 21-bp sequence that simultaneously abolished biological responsiveness to trans-activation by p40x and to stimulation by cAMP. We found that these mutations also eliminated the binding of the 180-kilodalton HeLa factor to the HTLV-I 21-bp element. In the absence of a demonstrable DNA-binding property for p40x, we hypothesize that cellular proteins are involved, possibly through signal transduction pathways, in its trans-activation of responsive promoters.

J Virol. 1988 December; 62(12): 4499-4509




This article has been cited by other articles:

  • Peloponese, J.-M. Jr., Yasunaga, J., Kinjo, T., Watashi, K., Jeang, K.-T. (2009). Peptidylproline cis-trans-Isomerase Pin1 Interacts with Human T-Cell Leukemia Virus Type 1 Tax and Modulates Its Activation of NF-{kappa}B. J. Virol. 83: 3238-3248 [Abstract] [Full Text]  
  • Ku, S. C. Y., Lee, J., Lau, J., Gurumurthy, M., Ng, R., Lwa, S. H., Lee, J., Klase, Z., Kashanchi, F., Chao, S.-H. (2008). XBP-1, a Novel Human T-Lymphotropic Virus Type 1 (HTLV-1) Tax Binding Protein, Activates HTLV-1 Basal and Tax-Activated Transcription. J. Virol. 82: 4343-4353 [Abstract] [Full Text]  
  • Lemasson, I., Lewis, M. R., Polakowski, N., Hivin, P., Cavanagh, M.-H., Thebault, S., Barbeau, B., Nyborg, J. K., Mesnard, J.-M. (2007). Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Protein Interacts with the Cellular Transcription Factor CREB To Inhibit HTLV-1 Transcription. J. Virol. 81: 1543-1553 [Abstract] [Full Text]  
  • Konesky, K. L., Nyborg, J. K., Laybourn, P. J. (2006). Tax Abolishes Histone H1 Repression of p300 Acetyltransferase Activity at the Human T-Cell Leukemia Virus Type 1 Promoter. J. Virol. 80: 10542-10553 [Abstract] [Full Text]  
  • Younis, I., Boris-Lawrie, K., Green, P. L. (2006). Human T-Cell Leukemia Virus Open Reading Frame II Encodes a Posttranscriptional Repressor That Is Recruited at the Level of Transcription. J. Virol. 80: 181-191 [Abstract] [Full Text]  
  • Twizere, J.-C., Lefebvre, L., Collete, D., Debacq, C., Urbain, P., Heremans, H., Jauniaux, J.-C., Burny, A., Willems, L., Kettmann, R. (2005). The Homeobox Protein MSX2 Interacts with Tax Oncoproteins and Represses Their Transactivation Activity. J. Biol. Chem. 280: 29804-29811 [Abstract] [Full Text]  
  • Alefantis, T., Mostoller, K., Jain, P., Harhaj, E., Grant, C., Wigdahl, B. (2005). Secretion of the Human T Cell Leukemia Virus Type I Transactivator Protein Tax. J. Biol. Chem. 280: 17353-17362 [Abstract] [Full Text]  
  • Jeang, K.-T., Giam, C.-z., Majone, F., Aboud, M. (2004). Life, Death, and Tax: Role of HTLV-I Oncoprotein in Genetic Instability and Cellular Transformation. J. Biol. Chem. 279: 31991-31994 [Full Text]  
  • Basbous, J., Bazarbachi, A., Granier, C., Devaux, C., Mesnard, J.-M. (2003). The Central Region of Human T-Cell Leukemia Virus Type 1 Tax Protein Contains Distinct Domains Involved in Subunit Dimerization. J. Virol. 77: 13028-13035 [Abstract] [Full Text]  
  • Dumais, N., Pare, M.-E., Mercier, S., Bounou, S., Marriot, S. J., Barbeau, B., Tremblay, M. J. (2003). T-Cell Receptor/CD28 Engagement When Combined with Prostaglandin E2 Treatment Leads to Potent Activation of Human T-Cell Leukemia Virus Type 1. J. Virol. 77: 11170-11179 [Abstract] [Full Text]  
  • Okada, M., Jeang, K.-T. (2002). Differential Requirements for Activation of Integrated and Transiently Transfected Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat. J. Virol. 76: 12564-12573 [Abstract] [Full Text]  
  • Gaudray, G., Gachon, F., Basbous, J., Biard-Piechaczyk, M., Devaux, C., Mesnard, J.-M. (2002). The Complementary Strand of the Human T-Cell Leukemia Virus Type 1 RNA Genome Encodes a bZIP Transcription Factor That Down-Regulates Viral Transcription. J. Virol. 76: 12813-12822 [Abstract] [Full Text]  
  • Mariner, J. M., Mamane, Y., Hiscott, J., Waldmann, T. A., Azimi, N. (2002). IFN Regulatory Factor 4 Participates in the Human T Cell Lymphotropic Virus Type I-Mediated Activation of the IL-15 Receptor {alpha} Promoter. J. Immunol. 168: 5667-5674 [Abstract] [Full Text]  
  • Smith, M. J., Gitlin, S. D., Browning, C. M., Lane, B. R., Clark, N. M., Shah, N., Rainier, S., Markovitz, D. M. (2001). GLI-2 Modulates Retroviral Gene Expression. J. Virol. 75: 2301-2313 [Abstract] [Full Text]  
  • Mariner, J. M., Lantz, V., Waldmann, T. A., Azimi, N. (2001). Human T Cell Lymphotropic Virus Type I Tax Activates IL-15R{{alpha}} Gene Expression Through an NF-{{kappa}}B Site. J. Immunol. 166: 2602-2609 [Abstract] [Full Text]  
  • Van, P. L., Yim, K.-W., Jin, D.-Y., Dapolito, G., Kurimasa, A., Jeang, K.-T. (2001). Genetic Evidence of a Role for ATM in Functional Interaction between Human T-Cell Leukemia Virus Type 1 Tax and p53. J. Virol. 75: 396-407 [Abstract] [Full Text]  
  • Datta, S., Kothari, N. H., Fan, H. (2000). In Vivo Genomic Footprinting of the Human T-Cell Leukemia Virus Type 1 (HTLV-1) Long Terminal Repeat Enhancer Sequences in HTLV-1-Infected Human T-Cell Lines with Different Levels of Tax I Activity. J. Virol. 74: 8277-8285 [Abstract] [Full Text]  
  • Gachon, F., Thebault, S., Peleraux, A., Devaux, C., Mesnard, J.-M. (2000). Molecular Interactions Involved in the Transactivation of the Human T-Cell Leukemia Virus Type 1 Promoter Mediated by Tax and CREB-2 (ATF-4). Mol. Cell. Biol. 20: 3470-3481 [Abstract] [Full Text]  
  • Ohtani, K., Iwanaga, R., Arai, M., Huang, Y., Matsumura, Y., Nakamura, M. (2000). Cell Type-specific E2F Activation and Cell Cycle Progression Induced by the Oncogene Product Tax of Human T-cell Leukemia Virus Type I. J. Biol. Chem. 275: 11154-11163 [Abstract] [Full Text]  
  • Ross, T. M., Narayan, M., Fang, Z.-Y., Minella, A. C., Green, P. L. (2000). Human T-Cell Leukemia Virus Type 2 Tax Mutants That Selectively Abrogate NFkappa B or CREB/ATF Activation Fail To Transform Primary Human T Cells. J. Virol. 74: 2655-2662 [Abstract] [Full Text]  
  • Anderson, M. G., Scoggin, K. E. S., Simbulan-Rosenthal, C. M., Steadman, J. A. (2000). Identification of Poly(ADP-Ribose) Polymerase as a Transcriptional Coactivator of the Human T-Cell Leukemia Virus Type 1 Tax Protein. J. Virol. 74: 2169-2177 [Abstract] [Full Text]  
  • Thebault, S., Gachon, F., Lemasson, I., Devaux, C., Mesnard, J.-M. (2000). Molecular Cloning of a Novel Human I-mfa Domain-containing Protein That Differently Regulates Human T-cell Leukemia Virus Type I and HIV-1 Expression. J. Biol. Chem. 275: 4848-4857 [Abstract] [Full Text]  
  • Kibler, K. V., Jeang, K.-T. (1999). Taxing the Cellular Capacity for Repair: Human T-Cell Leukemia Virus Type 1, DNA Damage, and Adult T-Cell Leukemia. JNCI J Natl Cancer Inst 91: 903-904 [Full Text]  
  • Lemasson, I., Thebault, S., Sardet, C., Devaux, C., Mesnard, J.-M. (1998). Activation of E2F-mediated Transcription by Human T-cell Leukemia Virus Type I Tax Protein in a p16INK4A-negative T-cell Line. J. Biol. Chem. 273: 23598-23604 [Abstract] [Full Text]  
  • Lin, H.-C., Dezzutti, C. S., Lal, R. B., Rabson, A. B. (1998). Activation of Human T-Cell Leukemia Virus Type 1 tax Gene Expression in Chronically Infected T Cells. J. Virol. 72: 6264-6270 [Abstract] [Full Text]  
  • Azimi, N., Brown, K., Bamford, R. N., Tagaya, Y., Siebenlist, U., Waldmann, T. A. (1998). Human T cell lymphotropic virus type I Tax protein trans-activates interleukin 15 gene transcription through an NF-kappa B site. Proc. Natl. Acad. Sci. USA 95: 2452-2457 [Abstract] [Full Text]  
  • Lenzmeier, B. A., Giebler, H. A., Nyborg, J. K. (1998). Human T-Cell Leukemia Virus Type 1 Tax Requires Direct Access to DNA for Recruitment of CREB Binding Protein to the Viral Promoter. Mol. Cell. Biol. 18: 721-731 [Abstract] [Full Text]  
  • Chun, R. F., Jeang, K.-T. (1996). Requirements for RNA Polymerase II Carboxyl-terminal Domain for Activated Transcription of Human Retroviruses Human T-Cell Lymphotropic Virus I and HIV-1. J. Biol. Chem. 271: 27888-27894 [Abstract] [Full Text]  
  • Semmes, O. J., Barrett, J. F., Dang, C. V., Jeang, K.-T. (1996). Human T-cell Leukemia Virus Type I Tax Masks c-Myc Function through a cAMP-dependent Pathway. J. Biol. Chem. 271: 9730-9738 [Abstract] [Full Text]  
  • Yin, M. J., Paulssen, E., Gaynor, R. B. (1996). cAMP-response Element-binding Protein Induces Directed DNA Bending of the HTLV-I 21-Base Pair Repeat. J. Biol. Chem. 271: 4781-4790 [Abstract] [Full Text]  
  • Kitajima, I., Kawahara, K., Hanyu, N., Shin, H., Tokioka, T., Soejima, Y., Tsutsui, J., Ozawa, M., Shimayama, T., Maruyama, I. (1996). Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I Tax-expressing PC12 cells. J. Cell Sci. 109: 609-617 [Abstract]  
  • Smith, M R, Greene, W C (1990). Identification of HTLV-I tax trans-activator mutants exhibiting novel transcriptional phenotypes.. Genes Dev. 4: 1875-1885 [Abstract]  
  • Jeang, K., Widen, S., Semmes, O. 4th, Wilson, S. (1990). HTLV-I trans-activator protein, tax, is a trans-repressor of the human beta-polymerase gene. Science 247: 1082-1084 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»