This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Koff, A Articles by Tegtmeyer, P Search for Related Content PubMed PubMed Citation Articles by Koff, A Articles by Tegtmeyer, P

 Previous Article  |  Next Article 

J Virol. 1988 November; 62(11): 4096-4103

Characterization of major recognition sequences for a herpes simplex virus type 1 origin-binding protein.

Department of Microbiology, State University of New York, Stony Brook 11794-8621.

ABSTRACT

To investigate early initiation events in the replication of herpes simplex virus type 1, we analyzed interactions of proteins from infected cell extracts with the small origin of herpes simplex virus type 1 (oris1). Using the mobility shift assay, we detected two origin-specific binding interactions. We characterized the more prominent interaction on both strands of the DNA duplex with DNase I protection and methylation interference assays. Protein binding protects 17 bases of DNA on each strand from DNase I. These sequences are located at the left end of the central palindrome and are shifted four bases relative to one another. On the basis of the DNase protection pattern, we believe this protein to be related to the origin-binding protein defined by Elias et al. (P. Elias, M.E. O'Donnell, E.S. Mocarski, and I.R. Lehman, Proc. Natl. Acad. Sci. 83:6322-6326, 1986). Our DNase I footprint shows both strong and weak areas of protection. The regions strongly protected from DNase I align with the essential contact residues identified by interference footprinting. Methylation interference defines a small binding domain of 8 base pairs: 5'-GTTCGCAC-3'/3'-CAAGCGTG-5'. This recognition sequence contains two inverted 5'-GT(T/G)CG-3' repeats which share a 2-base overlap; thus, the origin-binding protein probably binds to the inverted repeats as a dimer.

This article has been cited by other articles:

• Balliet, J. W., Min, J. C., Cabatingan, M. S., Schaffer, P. A. (2005). Site-Directed Mutagenesis of Large DNA Palindromes: Construction and In Vitro Characterization of Herpes Simplex Virus Type 1 Mutants Containing Point Mutations That Eliminate the oriL or oriS Initiation Function. J. Virol. 79: 12783-12797 [Abstract] [Full Text]  
• Fuchs, W., Ehrlich, C., Klupp, B. G., Mettenleiter, T. C. (2000). Characterization of the replication origin (OriS) and adjoining parts of the inverted repeat sequences of the pseudorabies virus genome. J. Gen. Virol. 81: 1539-1543 [Abstract] [Full Text]  
• Murata, L. B., Dodson, M. S. (1999). The Herpes Simplex Virus Type 1 Origin-binding Protein. SEQUENCE-SPECIFIC ACTIVATION OF ADENOSINE TRIPHOSPHATASE ACTIVITY BY A DOUBLE-STRANDED DNA CONTAINING BOX I. J. Biol. Chem. 274: 37079-37086 [Abstract] [Full Text]  
• Simonsson, S., Samuelsson, T., Elias, P. (1998). The Herpes Simplex Virus Type 1 Origin Binding Protein. SPECIFIC RECOGNITION OF PHOSPHATES AND METHYL GROUPS DEFINES THE INTERACTING SURFACE FOR A MONOMERIC DNA BINDING DOMAIN IN THE MAJOR GROOVE OF DNA. J. Biol. Chem. 273: 24633-24639 [Abstract] [Full Text]  
• Ziemann, K., Mettenleiter, T. C., Fuchs, W. (1998). Gene Arrangement within the Unique Long Genome Region of Infectious Laryngotracheitis Virus Is Distinct from That of Other Alphaherpesviruses. J. Virol. 72: 847-852 [Abstract] [Full Text]  
• Fierer, D. S., Fierer, D. S. (1995). The Stoichiometry of Binding of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, to Ori(S). J. Biol. Chem. 270: 7330-7334 [Abstract] [Full Text]