This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wechsler, S L Articles by Ghiasi, H Search for Related Content PubMed PubMed Citation Articles by Wechsler, S L Articles by Ghiasi, H

 Previous Article  |  Next Article 

J Virol. 1988 November; 62(11): 4051-4058

Fine mapping of the latency-related gene of herpes simplex virus type 1: alternative splicing produces distinct latency-related RNAs containing open reading frames.

Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, California 90048.

ABSTRACT

The latency-related (LR) gene of herpes simplex virus type 1 (HSV-1) is transcriptionally active during HSV-1 latency, producing at least two LR-RNAs. The LR gene partially overlaps the immediate-early gene ICP0 and is transcribed in the opposite direction from ICP0, producing LR-RNAs that are complementary (antisense) to ICP0 mRNA. The LR gene is thought to be involved in HSV-1 latency. We report here the fine mapping and partial sequence analysis of this HSV-1 LR gene. 32P-labeled genomic DNA restriction fragments and synthetic oligonucleotides were used as probes for in situ hybridizations and Northern (RNA) blot hybridizations of RNA from trigeminal ganglia of rabbits latently infected with HSV-1. The two most abundant LR-RNAs appeared to share their 5' and 3' ends and to be produced by alternative splicing. These LR-RNAs were approximately 2 and 1.3 to 1.5 kilobases in length and were designated LR-RNA 1 and LR-RNA 2, respectively. Their 5' ends started approximately 1,210 nucleotides downstream from the 3' end of the ICP0 mRNA. Their 3' ends overlapped ICP0 by nearly 1,000 nucleotides. LR-RNA 1 appeared to have at least one intron removed, while LR-RNA 2 appeared to have at least two introns removed. The LR-RNAs contained two potential long open reading frames, suggesting the possibility that one or more of the LR-RNAs may be a functional mRNA.

This article has been cited by other articles:

• Mott, K., Brick, D. J., van Rooijen, N., Ghiasi, H. (2007). Macrophages Are Important Determinants of Acute Ocular HSV-1 Infection in Immunized Mice. IOVS 48: 5605-5615 [Abstract] [Full Text]  
• Mott, K. R., Perng, G.-C., Osorio, Y., Kousoulas, K. G., Ghiasi, H. (2007). A Recombinant Herpes Simplex Virus Type 1 Expressing Two Additional Copies of gK Is More Pathogenic than Wild-Type Virus in Two Different Strains of Mice. J. Virol. 81: 12962-12972 [Abstract] [Full Text]  
• Jin, L., Perng, G.-C., Mott, K. R., Osorio, N., Naito, J., Brick, D. J., Carpenter, D., Jones, C., Wechsler, S. L. (2005). A Herpes Simplex Virus Type 1 Mutant Expressing a Baculovirus Inhibitor of Apoptosis Gene in Place of Latency-Associated Transcript Has a Wild-Type Reactivation Phenotype in the Mouse. J. Virol. 79: 12286-12295 [Abstract] [Full Text]  
• Mott, K. R., Osorio, N., Jin, L., Brick, D. J., Naito, J., Cooper, J., Henderson, G., Inman, M., Jones, C., Wechsler, S. L., Perng, G.-C. (2003). The bovine herpesvirus-1 LR ORF2 is critical for this gene's ability to restore the high wild-type reactivation phenotype to a herpes simplex virus-1 LAT null mutant. J. Gen. Virol. 84: 2975-2985 [Abstract] [Full Text]  
• Jin, L., Peng, W., Perng, G.-C., Brick, D. J., Nesburn, A. B., Jones, C., Wechsler, S. L. (2003). Identification of Herpes Simplex Virus Type 1 Latency-Associated Transcript Sequences That both Inhibit Apoptosis and Enhance the Spontaneous Reactivation Phenotype. J. Virol. 77: 6556-6561 [Abstract] [Full Text]  
• Burton, E. A., Hong, C.-S., Glorioso, J. C. (2003). The Stable 2.0-Kilobase Intron of the Herpes Simplex Virus Type 1 Latency-Associated Transcript Does Not Function as an Antisense Repressor of ICP0 in Nonneuronal Cells. J. Virol. 77: 3516-3530 [Abstract] [Full Text]  
• Perng, G.-C., Maguen, B., Jin, L., Mott, K. R., Kurylo, J., BenMohamed, L., Yukht, A., Osorio, N., Nesburn, A. B., Henderson, G., Inman, M., Jones, C., Wechsler, S. L. (2002). A Novel Herpes Simplex Virus Type 1 Transcript (AL-RNA) Antisense to the 5' End of the Latency-Associated Transcript Produces a Protein in Infected Rabbits. J. Virol. 76: 8003-8010 [Abstract] [Full Text]  
• Perng, G.-C., Maguen, B., Jin, L., Mott, K. R., Osorio, N., Slanina, S. M., Yukht, A., Ghiasi, H., Nesburn, A. B., Inman, M., Henderson, G., Jones, C., Wechsler, S. L. (2002). A Gene Capable of Blocking Apoptosis Can Substitute for the Herpes Simplex Virus Type 1 Latency-Associated Transcript Gene and Restore Wild-Type Reactivation Levels. J. Virol. 76: 1224-1235 [Abstract] [Full Text]  
• Thomas, D. L., Lock, M., Zabolotny, J. M., Mohan, B. R., Fraser, N. W. (2002). The 2-Kilobase Intron of the Herpes Simplex Virus Type 1 Latency-Associated Transcript Has a Half-Life of Approximately 24 Hours in SY5Y and COS-1 Cells. J. Virol. 76: 532-540 [Abstract] [Full Text]  
• Taus, N. S., Mitchell, W. J. (2001). The Transgenic ICP4 Promoter Is Activated in Schwann Cells in Trigeminal Ganglia of Mice Latently Infected with Herpes Simplex Virus Type 1. J. Virol. 75: 10401-10408 [Abstract] [Full Text]  
• Perng, G.-C., Esmaili, D., Slanina, S. M., Yukht, A., Ghiasi, H., Osorio, N., Mott, K. R., Maguen, B., Jin, L., Nesburn, A. B., Wechsler, S. L. (2001). Three Herpes Simplex Virus Type 1 Latency-Associated Transcript Mutants with Distinct and Asymmetric Effects on Virulence in Mice Compared with Rabbits. J. Virol. 75: 9018-9028 [Abstract] [Full Text]  
• Berthomme, H., Thomas, J., Texier, P., Epstein, A., Feldman, L. T. (2001). Enhancer and Long-Term Expression Functions of Herpes Simplex Virus Type 1 Latency-Associated Promoter Are both Located in the Same Region. J. Virol. 75: 4386-4393 [Abstract] [Full Text]  
• Lock, M., Miller, C., Fraser, N. W. (2001). Analysis of Protein Expression from within the Region Encoding the 2.0-Kilobase Latency-Associated Transcript of Herpes Simplex Virus Type 1. J. Virol. 75: 3413-3426 [Abstract] [Full Text]  
• Berthomme, H., Lokensgard, J., Yang, L., Margolis, T., Feldman, L. T. (2000). Evidence for a Bidirectional Element Located Downstream from the Herpes Simplex Virus Type 1 Latency-Associated Promoter That Increases Its Activity during Latency. J. Virol. 74: 3613-3622 [Abstract] [Full Text]  
• Perng, G.-C., Slanina, S. M., Yukht, A., Ghiasi, H., Nesburn, A. B., Wechsler, S. L. (2000). The Latency-Associated Transcript Gene Enhances Establishment of Herpes Simplex Virus Type 1 Latency in Rabbits. J. Virol. 74: 1885-1891 [Abstract] [Full Text]  
• Alvira, M. R., Goins, W. F., Cohen, J. B., Glorioso, J. C. (1999). Genetic Studies Exposing the Splicing Events Involved in Herpes Simplex Virus Type 1 Latency-Associated Transcript Production during Lytic and Latent Infection. J. Virol. 73: 3866-3876 [Abstract] [Full Text]  
• Perng, G.-C., Slanina, S. M., Yukht, A., Drolet, B. S., Keleher, W. Jr., Ghiasi, H., Nesburn, A. B., Wechsler, S. L. (1999). A Herpes Simplex Virus Type 1 Latency-Associated Transcript Mutant with Increased Virulence and Reduced Spontaneous Reactivation. J. Virol. 73: 920-929 [Abstract] [Full Text]  
• Loutsch, J. M., Perng, G.-C., Hill, J. M., Zheng, X., Marquart, M. E., Block, T. M., Ghiasi, H., Nesburn, A. B., Wechsler, S. L. (1999). Identical 371-Base-Pair Deletion Mutations in the LAT Genes of Herpes Simplex Virus Type 1 McKrae and 17syn+ Result in Different In Vivo Reactivation Phenotypes. J. Virol. 73: 767-771 [Abstract] [Full Text]  
• Ziemann, K., Mettenleiter, T. C., Fuchs, W. (1998). Infectious Laryngotracheitis Herpesvirus Expresses a Related Pair of Unique Nuclear Proteins Which Are Encoded by Split Genes Located at the Right End of the UL Genome Region. J. Virol. 72: 6867-6874 [Abstract] [Full Text]