This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mervis, R J Articles by Venkatesan, S Search for Related Content PubMed PubMed Citation Articles by Mervis, R J Articles by Venkatesan, S

 Previous Article  |  Next Article 

J Virol. 1988 November; 62(11): 3993-4002

The gag gene products of human immunodeficiency virus type 1: alignment within the gag open reading frame, identification of posttranslational modifications, and evidence for alternative gag precursors.

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

ABSTRACT

Seven human immunodeficiency virus gag polypeptides were identified in the purified virus and in infected CD4+ lymphocytes by peptide mapping and limited amino acid sequencing of immune-purified proteins. Two gag polyproteins of 55,000 (p55) and 41,000 (p41) daltons were rapidly labeled and readily processed into the major internal gag proteins that were aligned within the gag open reading frame (ORF) as NH2-p16 (MA)-p24 (CA)-p9 (NC)-p7-COOH. The myristoylated p16 (matrix, MA) protein was processed from the myristoylated p55 gag precursor protein. The immunoreactivity of the p16 (MA) protein with region-specific gag antisera and the conservation of the N-terminal myristyl group of the p55 precursor protein in p16 (MA) confirmed its position as the N-terminal-most protein. The p9 (nucleocapsid, NC) protein was localized to residue 378 of the gag ORF, next to the C terminus of the p24/p25 (core antigen, CA) protein. The p9 protein had a repeating Cys residue containing motif which is found in the nucleic acid-binding Cys residue-containing proteins of retroviruses. The p24 (CA) protein, which was localized to residue 133 of the gag ORF, was apparently derived by C-terminal processing of an intermediate polypeptide, p25. Both the mature p24 (CA) and p16 (MA) proteins were phosphorylated at Ser residue(s). We also identified two forms of gag p41 species, one resulting from the C-terminal processing of p55 and the other originating either from N-terminal processing of p55 or from de novo synthesis.

This article has been cited by other articles:

• Liao, W.-H., Huang, K.-J., Chang, Y.-F., Wang, S.-M., Tseng, Y.-T., Chiang, C.-C., Wang, J.-J., Wang, C.-T. (2007). Incorporation of Human Immunodeficiency Virus Type 1 Reverse Transcriptase into Virus-Like Particles. J. Virol. 81: 5155-5165 [Abstract] [Full Text]  
• Adamson, C. S., Ablan, S. D., Boeras, I., Goila-Gaur, R., Soheilian, F., Nagashima, K., Li, F., Salzwedel, K., Sakalian, M., Wild, C. T., Freed, E. O. (2006). In Vitro Resistance to the Human Immunodeficiency Virus Type 1 Maturation Inhibitor PA-457 (Bevirimat). J. Virol. 80: 10957-10971 [Abstract] [Full Text]  
• Chiu, H.-C., Wang, F.-D., Chen, Y.-M. A., Wang, C.-T. (2006). Effects of human immunodeficiency virus type 1 transframe protein p6* mutations on viral protease-mediated Gag processing. J. Gen. Virol. 87: 2041-2046 [Abstract] [Full Text]  
• Pettit, S. C., Clemente, J. C., Jeung, J. A., Dunn, B. M., Kaplan, A. H. (2005). Ordered Processing of the Human Immunodeficiency Virus Type 1 GagPol Precursor Is Influenced by the Context of the Embedded Viral Protease. J. Virol. 79: 10601-10607 [Abstract] [Full Text]  
• Rudner, L., Nydegger, S., Coren, L. V., Nagashima, K., Thali, M., Ott, D. E. (2005). Dynamic Fluorescent Imaging of Human Immunodeficiency Virus Type 1 Gag in Live Cells by Biarsenical Labeling. J. Virol. 79: 4055-4065 [Abstract] [Full Text]  
• Rue, S. M., Roos, J. W., Tarwater, P. M., Clements, J. E., Barber, S. A. (2005). Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus. J. Virol. 79: 2484-2492 [Abstract] [Full Text]  
• Guo, X., Roldan, A., Hu, J., Wainberg, M. A., Liang, C. (2005). Mutation of the SP1 Sequence Impairs both Multimerization and Membrane-Binding Activities of Human Immunodeficiency Virus Type 1 Gag. J. Virol. 79: 1803-1812 [Abstract] [Full Text]  
• Beausejour, Y., Tremblay, M. J. (2004). Interaction between the Cytoplasmic Domain of ICAM-1 and Pr55Gag Leads to Acquisition of Host ICAM-1 by Human Immunodeficiency Virus Type 1. J. Virol. 78: 11916-11925 [Abstract] [Full Text]  
• Pettit, S. C., Everitt, L. E., Choudhury, S., Dunn, B. M., Kaplan, A. H. (2004). Initial Cleavage of the Human Immunodeficiency Virus Type 1 GagPol Precursor by Its Activated Protease Occurs by an Intramolecular Mechanism. J. Virol. 78: 8477-8485 [Abstract] [Full Text]  
• Myint, L., Matsuda, M., Matsuda, Z., Yokomaku, Y., Chiba, T., Okano, A., Yamada, K., Sugiura, W. (2004). Gag Non-Cleavage Site Mutations Contribute to Full Recovery of Viral Fitness in Protease Inhibitor-Resistant Human Immunodeficiency Virus Type 1. Antimicrob. Agents Chemother. 48: 444-452 [Abstract] [Full Text]  
• Tang, S., Murakami, T., Cheng, N., Steven, A. C., Freed, E. O., Levin, J. G. (2003). Human Immunodeficiency Virus Type 1 N-Terminal Capsid Mutants Containing Cores with Abnormally High Levels of Capsid Protein and Virtually No Reverse Transcriptase. J. Virol. 77: 12592-12602 [Abstract] [Full Text]  
• Li, F., Goila-Gaur, R., Salzwedel, K., Kilgore, N. R., Reddick, M., Matallana, C., Castillo, A., Zoumplis, D., Martin, D. E., Orenstein, J. M., Allaway, G. P., Freed, E. O., Wild, C. T. (2003). PA-457: A potent HIV inhibitor that disrupts core condensation by targeting a late step in Gag processing. Proc. Natl. Acad. Sci. USA 100: 13555-13560 [Abstract] [Full Text]  
• Lyonnais, S., Gorelick, R. J., Mergny, J.-L., Le Cam, E., Mirambeau, G. (2003). G-quartets direct assembly of HIV-1 nucleocapsid protein along single-stranded DNA. Nucleic Acids Res 31: 5754-5763 [Abstract] [Full Text]  
• Rue, S. M., Roos, J. W., Amzel, L. M., Clements, J. E., Barber, S. A. (2003). Hydrogen Bonding at a Conserved Threonine in Lentivirus Capsid Is Required for Virus Replication. J. Virol. 77: 8009-8018 [Abstract] [Full Text]  
• Pettit, S. C., Gulnik, S., Everitt, L., Kaplan, A. H. (2002). The Dimer Interfaces of Protease and Extra-Protease Domains Influence the Activation of Protease and the Specificity of GagPol Cleavage. J. Virol. 77: 366-374 [Abstract] [Full Text]  
• Pettit, S. C., Henderson, G. J., Schiffer, C. A., Swanstrom, R. (2002). Replacement of the P1 Amino Acid of Human Immunodeficiency Virus Type 1 Gag Processing Sites Can Inhibit or Enhance the Rate of Cleavage by the Viral Protease. J. Virol. 76: 10226-10233 [Abstract] [Full Text]  
• Lanman, J., Sexton, J., Sakalian, M., Prevelige, P. E. Jr. (2002). Kinetic Analysis of the Role of Intersubunit Interactions in Human Immunodeficiency Virus Type 1 Capsid Protein Assembly In Vitro. J. Virol. 76: 6900-6908 [Abstract] [Full Text]  
• Guo, J., Wu, T., Kane, B. F., Johnson, D. G., Henderson, L. E., Gorelick, R. J., Levin, J. G. (2002). Subtle Alterations of the Native Zinc Finger Structures Have Dramatic Effects on the Nucleic Acid Chaperone Activity of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein. J. Virol. 76: 4370-4378 [Abstract] [Full Text]  
• Chiu, H.-C., Yao, S.-Y., Wang, C.-T. (2002). Coding Sequences Upstream of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase Domain in Gag-Pol Are Not Essential for Incorporation of the Pr160gag-pol into Virus Particles. J. Virol. 76: 3221-3231 [Abstract] [Full Text]  
• Muller, B., Patschinsky, T., Krausslich, H.-G. (2002). The Late-Domain-Containing Protein p6 Is the Predominant Phosphoprotein of Human Immunodeficiency Virus Type 1 Particles. J. Virol. 76: 1015-1024 [Abstract] [Full Text]  
• Demirov, D. G., Orenstein, J. M., Freed, E. O. (2002). The Late Domain of Human Immunodeficiency Virus Type 1 p6 Promotes Virus Release in a Cell Type-Dependent Manner. J. Virol. 76: 105-117 [Abstract] [Full Text]  
• Bardy, M., Gay, B., Pebernard, S., Chazal, N., Courcoul, M., Vigne, R., Decroly, E., Boulanger, P. (2001). Interaction of human immunodeficiency virus type 1 Vif with Gag and Gag-Pol precursors: co-encapsidation and interference with viral protease-mediated Gag processing. J. Gen. Virol. 82: 2719-2733 [Abstract] [Full Text]  
• Tang, S., Murakami, T., Agresta, B. E., Campbell, S., Freed, E. O., Levin, J. G. (2001). Human Immunodeficiency Virus Type 1 N-Terminal Capsid Mutants That Exhibit Aberrant Core Morphology and Are Blocked in Initiation of Reverse Transcription in Infected Cells. J. Virol. 75: 9357-9366 [Abstract] [Full Text]  
• Beck, Z. Q., Lin, Y.-C., Elder, J. H. (2001). Molecular Basis for the Relative Substrate Specificity of Human Immunodeficiency Virus Type 1 and Feline Immunodeficiency Virus Proteases. J. Virol. 75: 9458-9469 [Abstract] [Full Text]  
• Zheng, L., Zhang, S., Wood, C., Kapil, S., Wilcox, G. E., Loughin, T. A., Minocha, H. C. (2001). Differentiation of Two Bovine Lentiviruses by a Monoclonal Antibody on the Basis of Epitope Specificity. CVI 8: 283-287 [Abstract] [Full Text]  
• Müller, B., Tessmer, U., Schubert, U., Kräusslich, H.-G. (2000). Human Immunodeficiency Virus Type 1 Vpr Protein Is Incorporated into the Virion in Significantly Smaller Amounts than Gag and Is Phosphorylated in Infected Cells. J. Virol. 74: 9727-9731 [Abstract] [Full Text]  
• Zheng, L., Swanson, M., Liao, J., Wood, C., Kapil, S., Snider, R., Loughin, T. A., Minocha, H. C. (2000). Cloning of the Bovine Immunodeficiency Virus gag Gene and Development of a Recombinant-Protein-Based Enzyme-Linked Immunosorbent Assay. CVI 7: 557-562 [Abstract] [Full Text]  
• Wang, C.-T., Chou, Y.-C., Chiang, C.-C. (2000). Assembly and Processing of Human Immunodeficiency Virus Gag Mutants Containing a Partial Replacement of the Matrix Domain by the Viral Protease Domain. J. Virol. 74: 3418-3422 [Abstract] [Full Text]  
• Guerra-Peraza, O., de Tapia, M., Hohn, T., Hemmings-Mieszczak, M. (2000). Interaction of the Cauliflower Mosaic Virus Coat Protein with the Pregenomic RNA Leader. J. Virol. 74: 2067-2072 [Abstract] [Full Text]  
• Morikawa, Y., Hockley, D. J., Nermut, M. V., Jones, I. M. (2000). Roles of Matrix, p2, and N-Terminal Myristoylation in Human Immunodeficiency Virus Type 1 Gag Assembly. J. Virol. 74: 16-23 [Abstract] [Full Text]  
• Morikawa, Y., Goto, T., Sano, K. (1999). In Vitro Assembly of Human Immunodeficiency Virus Type 1 Gag Protein. J. Biol. Chem. 274: 27997-28002 [Abstract] [Full Text]  
• Cartier, C., Sivard, P., Tranchat, C., Decimo, D., Desgranges, C., Boyer, V. (1999). Identification of Three Major Phosphorylation Sites within HIV-1 Capsid. ROLE OF PHOSPHORYLATION DURING THE EARLY STEPS OF INFECTION. J. Biol. Chem. 274: 19434-19440 [Abstract] [Full Text]  
• Borsetti, A., Ohagen, A., Gottlinger, H. G. (1998). The C-Terminal Half of the Human Immunodeficiency Virus Type 1 Gag Precursor Is Sufficient for Efficient Particle Assembly. J. Virol. 72: 9313-9317 [Abstract] [Full Text]  
• Wang, C.-T., Lai, H.-Y., Li, J.-J. (1998). Analysis of Minimal Human Immunodeficiency Virus Type 1 gag Coding Sequences Capable of Virus-Like Particle Assembly and Release. J. Virol. 72: 7950-7959 [Abstract] [Full Text]  
• Yu, Q., Ottmann, M., Pechoux, C., Le Grice, S., Darlix, J.-L. (1998). Mutations in the Primer Grip of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Impair Proviral DNA Synthesis and Virion Maturation. J. Virol. 72: 7676-7680 [Abstract] [Full Text]  
• Gross, I., Hohenberg, H., Huckhagel, C., Krausslich, H.-G. (1998). N-Terminal Extension of Human Immunodeficiency Virus Capsid Protein Converts the In Vitro Assembly Phenotype from Tubular to Spherical Particles. J. Virol. 72: 4798-4810 [Abstract] [Full Text]  
• Kong, L. B., An, D., Ackerson, B., Canon, J., Rey, O., Chen, I. S. Y., Krogstad, P., Stewart, P. L. (1998). Cryoelectron Microscopic Examination of Human Immunodeficiency Virus Type 1 Virions with Mutations in the Cyclophilin A Binding Loop. J. Virol. 72: 4403-4407 [Abstract] [Full Text]  
• Wiegers, K., Rutter, G., Kottler, H., Tessmer, U., Hohenberg, H., Krausslich, H.-G. (1998). Sequential Steps in Human Immunodeficiency Virus Particle Maturation Revealed by Alterations of Individual Gag Polyprotein Cleavage Sites. J. Virol. 72: 2846-2854 [Abstract] [Full Text]  
• Zhang, Y., Qian, H., Love, Z., Barklis, E. (1998). Analysis of the Assembly Function of the Human Immunodeficiency Virus Type 1 Gag Protein Nucleocapsid Domain. J. Virol. 72: 1782-1789 [Abstract] [Full Text]  
• Accola, M. A., Hoglund, S., Gottlinger, H. G. (1998). A Putative alpha -Helical Structure Which Overlaps the Capsid-p2 Boundary in the Human Immunodeficiency Virus Type 1 Gag Precursor Is Crucial for Viral Particle Assembly. J. Virol. 72: 2072-2078 [Abstract] [Full Text]  
• De Guzman, R. N., Wu, Z. R., Stalling, C. C., Pappalardo, L., Borer, P. N., Summers, M. F. (1998). Structure of the HIV-1 Nucleocapsid Protein Bound to the SL3 -RNA Recognition Element. Science 279: 384-388 [Abstract] [Full Text]  
• Rasnick, D., Rasnick, D. (1997). Kinetics Analysis of Consecutive HIV Proteolytic Cleavages of the Gag-Pol Polyprotein. J. Biol. Chem. 272: 6348-6353 [Abstract] [Full Text]  
• Yang, X., Goncalves, J., Gabuzda, D. (1996). Phosphorylation of Vif and Its Role in HIV-1 Replication. J. Biol. Chem. 271: 10121-10129 [Abstract] [Full Text]