This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tollefson, A E Articles by Wold, W S Search for Related Content PubMed PubMed Citation Articles by Tollefson, A E Articles by Wold, W S

 Previous Article  |  Next Article 

J Virol. 1988 January; 62(1): 33-39

Identification and gene mapping of a 14,700-molecular-weight protein encoded by region E3 of group C adenoviruses.

Institute for Molecular Virology, St. Louis University School of Medicine, Missouri 63110.

ABSTRACT

Early region E3 of adenovirus type 5 should encode at least nine proteins as judged by the DNA sequence and the spliced structures of the known mRNAs. Only two E3 proteins have been proved to exist, a glycoprotein (gp19K) and an 11,600-molecular-weight protein (11.6K protein). Here we describe an abundant 14.7K protein coded by a gene in the extreme 3' portion of E3. To identify this 14.7K protein, we constructed a bacterial vector which synthesized a TrpE-14.7K fusion protein, then we prepared antiserum against the fusion protein. This antiserum immunoprecipitated the 14.7K protein from cells infected with adenovirus types 5 and 2, as well as with a variety of E3 deletion mutants. Synthesis of the 14.7K protein correlated precisely with the presence or absence of the 14.7K gene and with the synthesis of the mRNA (mRNA h) which encodes the 14.7K protein. The 14.7K protein appeared as a triplet on immunoprecipitation gels and Western blots (immunoblots).

This article has been cited by other articles:

• Lichtenstein, D. L., Doronin, K., Toth, K., Kuppuswamy, M., Wold, W. S. M., Tollefson, A. E. (2004). Adenovirus E3-6.7K Protein Is Required in Conjunction with the E3-RID Protein Complex for the Internalization and Degradation of TRAIL Receptor 2. J. Virol. 78: 12297-12307 [Abstract] [Full Text]  
• Zanardi, T. A., Yei, S., Lichtenstein, D. L., Tollefson, A. E., Wold, W. S. M. (2003). Distinct Domains in the Adenovirus E3 RID{alpha} Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor. J. Virol. 77: 11685-11696 [Abstract] [Full Text]  
• Tollefson, A. E., Scaria, A., Ying, B., Wold, W. S. M. (2003). Mutations within the ADP (E3-11.6K) Protein Alter Processing and Localization of ADP and the Kinetics of Cell Lysis of Adenovirus-Infected Cells. J. Virol. 77: 7764-7778 [Abstract] [Full Text]  
• Kim, H.-J., Foster, M. P. (2002). Characterization of Ad5 E3-14.7K, an adenoviral inhibitor of apoptosis: Structure, oligomeric state, and metal binding. Protein Sci. 11: 1117-1128 [Abstract] [Full Text]  
• Tollefson, A. E., Toth, K., Doronin, K., Kuppuswamy, M., Doronina, O. A., Lichtenstein, D. L., Hermiston, T. W., Smith, C. A., Wold, W. S. M. (2001). Inhibition of TRAIL-Induced Apoptosis and Forced Internalization of TRAIL Receptor 1 by Adenovirus Proteins. J. Virol. 75: 8875-8887 [Abstract] [Full Text]