This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Doermann, A H Articles by Jackson, P Search for Related Content PubMed PubMed Citation Articles by Doermann, A H Articles by Jackson, P

Genetic control of capsid length in bacteriophage T4: clustering of ptg mutations in gene 23.

ABSTRACT

Fifty-two new bacteriophage T4 ptg mutations have been isolated by selecting for the giant-capsid phenotype they display. Genetic mapping placed all of them at eight sites, all located in gene 23. These sites were clustered in three locations, one near amber B17 (gene 23 nucleotide [NT] 268), another centrally placed between amE506 (NT 706) and amE1270 (NT 925), and the third between amC208 (NT 1297) and amE1236 (NT 1489). The lack of a selective system for identifying recombinant genotypes when dealing with the very close linkages found within these clusters opens the possibility that more than eight sites are represented in this set of mutations. Since one site was represented by only one mutation, it seems likely that further searching might uncover additional sites. It is suggested that the clustering of mutations observed here identifies regions of the gene 23 product that play a role in regulating the capsid length of T4.

This article has been cited by other articles:

• Fokine, A., Leiman, P. G., Shneider, M. M., Ahvazi, B., Boeshans, K. M., Steven, A. C., Black, L. W., Mesyanzhinov, V. V., Rossmann, M. G. (2005). Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry. Proc. Natl. Acad. Sci. USA 102: 7163-7168 [Abstract] [Full Text]  
• Tétart, F., Desplats, C., Kutateladze, M., Monod, C., Ackermann, H.-W., Krisch, H. M. (2001). Phylogeny of the Major Head and Tail Genes of the Wide-Ranging T4-Type Bacteriophages. J. Bacteriol. 183: 358-366 [Abstract] [Full Text]