This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hearing, P Articles by Shenk, T Search for Related Content PubMed PubMed Citation Articles by Hearing, P Articles by Shenk, T

 Previous Article  |  Next Article 

J Virol. 1987 August; 61(8): 2555-2558

Identification of a repeated sequence element required for efficient encapsidation of the adenovirus type 5 chromosome.

ABSTRACT

Adenovirus type 5 deletion mutants that lack portions of their cis-acting DNA encapsidation signal synthesized nearly normal levels of viral DNA and late polypeptides but failed to efficiently package the DNA into virus particles. A series of mutant viruses carrying small deletions were produced and used to identify a repeated element (AGTAAATTTGGGC and AGTAAGATTTGGCC) as a key component of the packaging signal. One copy of the repeat was sufficient to signal efficient packaging. The packaging domain could function near either end of the viral chromosome but was no longer active when moved several hundred base pairs toward the interior of the DNA molecule.

This article has been cited by other articles:

• Christensen, J. B., Byrd, S. A., Walker, A. K., Strahler, J. R., Andrews, P. C., Imperiale, M. J. (2008). Presence of the Adenovirus IVa2 Protein at a Single Vertex of the Mature Virion. J. Virol. 82: 9086-9093 [Abstract] [Full Text]  
• Ewing, S. G., Byrd, S. A., Christensen, J. B., Tyler, R. E., Imperiale, M. J. (2007). Ternary Complex Formation on the Adenovirus Packaging Sequence by the IVa2 and L4 22-Kilodalton Proteins. J. Virol. 81: 12450-12457 [Abstract] [Full Text]  
• Tyler, R. E., Ewing, S. G., Imperiale, M. J. (2007). Formation of a Multiple Protein Complex on the Adenovirus Packaging Sequence by the IVa2 Protein. J. Virol. 81: 3447-3454 [Abstract] [Full Text]  
• Ostapchuk, P., Anderson, M. E., Chandrasekhar, S., Hearing, P. (2006). The L4 22-Kilodalton Protein Plays a Role in Packaging of the Adenovirus Genome. J. Virol. 80: 6973-6981 [Abstract] [Full Text]  
• Perez-Romero, P., Gustin, K. E., Imperiale, M. J. (2006). Dependence of the Encapsidation Function of the Adenovirus L1 52/55-Kilodalton Protein on Its Ability To Bind the Packaging Sequence. J. Virol. 80: 1965-1971 [Abstract] [Full Text]  
• Ostapchuk, P., Yang, J., Auffarth, E., Hearing, P. (2005). Functional Interaction of the Adenovirus IVa2 Protein with Adenovirus Type 5 Packaging Sequences. J. Virol. 79: 2831-2838 [Abstract] [Full Text]  
• Perez-Romero, P., Tyler, R. E., Abend, J. R., Dus, M., Imperiale, M. J. (2005). Analysis of the Interaction of the Adenovirus L1 52/55-Kilodalton and IVa2 Proteins with the Packaging Sequence In Vivo and In Vitro. J. Virol. 79: 2366-2374 [Abstract] [Full Text]  
• Murakami, P., Havenga, M., Fawaz, F., Vogels, R., Marzio, G., Pungor, E., Files, J., Do, L., Goudsmit, J., McCaman, M. (2004). Common Structure of Rare Replication-Deficient E1-Positive Particles in Adenoviral Vector Batches. J. Virol. 78: 6200-6208 [Abstract] [Full Text]  
• Xing, L., Zhang, L., Kessel, J. V., Tikoo, S. K. (2003). Identification of cis-acting sequences required for selective packaging of bovine adenovirus type 3 DNA. J. Gen. Virol. 84: 2947-2956 [Abstract] [Full Text]  
• Erturk, E., Ostapchuk, P., Wells, S. I., Yang, J., Gregg, K., Nepveu, A., Dudley, J. P., Hearing, P. (2003). Binding of CCAAT Displacement Protein CDP to Adenovirus Packaging Sequences. J. Virol. 77: 6255-6264 [Abstract] [Full Text]  
• Ostapchuk, P., Hearing, P. (2003). Minimal cis-Acting Elements Required for Adenovirus Genome Packaging. J. Virol. 77: 5127-5135 [Abstract] [Full Text]  
• Sandalon, Z., Gnatenko, D. V., Bahou, W. F., Hearing, P. (2000). Adeno-Associated Virus (AAV) Rep Protein Enhances the Generation of a Recombinant Mini-Adenovirus (Ad) Utilizing an Ad/AAV Hybrid Virus. J. Virol. 74: 10381-10389 [Abstract] [Full Text]  
• Russell, W. C. (2000). Update on adenovirus and its vectors. J. Gen. Virol. 81: 2573-2604 [Full Text]  
• Qin, S., Ward, B. M., Lazarowitz, S. G. (1998). The Bipartite Geminivirus Coat Protein Aids BR1 Function in Viral Movement by Affecting the Accumulation of Viral Single-Stranded DNA. J. Virol. 72: 9247-9256 [Abstract] [Full Text]  
• Schmid, S. I., Hearing, P. (1998). Cellular Components Interact with Adenovirus Type 5 Minimal DNA Packaging Domains. J. Virol. 72: 6339-6347 [Abstract] [Full Text]  
• Franz, W.-M, Rothmann, T., Frey, N., Katus, H. A (1997). Analysis of tissue-specific gene delivery by recombinant adenoviruses containing cardiac-specific promoters. Cardiovasc Res 35: 560-566 [Abstract] [Full Text]