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Intracellular digestion of reovirus particles requires a low pH and is an essential step in the viral infectious cycle.

ABSTRACT

Lysosomotropic drugs such as NH4Cl have been useful for studying the role of low pH in early events in virus infection. NH4Cl blocks the production of infectious progeny virus in mammalian reovirus-infected cells. The inhibitory effect of NH4Cl is mediated by an inhibition of intracellular digestion of reovirus outer capsid proteins. In vitro digestion of viral outer capsid proteins produces infectious partially uncoated particles, called intermediate subviral particles, which are no longer inhibited by the presence of NH4Cl. These results indicate that proteolytic processing of reovirus outer capsid proteins takes place in a low pH compartment of the cell and is an essential step in the viral infectious cycle.

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• Baer, G. S., Ebert, D. H., Chung, C. J., Erickson, A. H., Dermody, T. S. (1999). Mutant Cells Selected during Persistent Reovirus Infection Do Not Express Mature Cathepsin L and Do Not Support Reovirus Disassembly. J. Virol. 73: 9532-9543 [Abstract] [Full Text]  
• Chandran, K., Walker, S. B., Chen, Y., Contreras, C. M., Schiff, L. A., Baker, T. S., Nibert, M. L. (1999). In Vitro Recoating of Reovirus Cores with Baculovirus-Expressed Outer-Capsid Proteins µ1 and sigma 3. J. Virol. 73: 3941-3950 [Abstract] [Full Text]  
• Jané-Valbuena, J., Nibert, M. L., Spencer, S. M., Walker, S. B., Baker, T. S., Chen, Y., Centonze, V. E., Schiff, L. A. (1999). Reovirus Virion-Like Particles Obtained by Recoating Infectious Subvirion Particles with Baculovirus-Expressed sigma 3 Protein: an Approach for Analyzing sigma 3 Functions during Virus Entry. J. Virol. 73: 2963-2973 [Abstract] [Full Text]  
• Chappell, J. D., Barton, E. S., Smith, T. H., Baer, G. S., Duong, D. T., Nibert, M. L., Dermody, T. S. (1998). Cleavage Susceptibility of Reovirus Attachment Protein sigma 1 during Proteolytic Disassembly of Virions Is Determined by a Sequence Polymorphism in the sigma 1 Neck. J. Virol. 72: 8205-8213 [Abstract] [Full Text]  
• Chandran, K., Nibert, M. L. (1998). Protease Cleavage of Reovirus Capsid Protein µ1/µ1C Is Blocked by Alkyl Sulfate Detergents, Yielding a New Type of Infectious Subvirion Particle. J. Virol. 72: 467-475 [Abstract] [Full Text]