This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hertz, G Z Articles by Mertz, J E Search for Related Content PubMed PubMed Citation Articles by Hertz, G Z Articles by Mertz, J E

The A+T-rich sequence of the simian virus 40 origin is essential for replication and is involved in bending of the viral DNA.

ABSTRACT

The origin-promoter region of simian virus 40 contains a 17-base-pair sequence composed exclusively of adenine (A) and thymine (T). We constructed a linker replacement mutant in which this stretch of A's and T's was reduced to 11 base pairs. While not affecting the level of early gene transcription, this mutation reduced the accumulation of viral DNA in COS cells at least 10(4) fold. In addition, a restriction fragment containing the wild-type A + T-rich region migrated in nondenaturing polyacrylamide gels with an anomalous mobility characteristic of bent DNA; however, the corresponding fragment from the mutant migrated less anomalously. Therefore, bending of the DNA in this region may play a role in some step in viral DNA replication.

This article has been cited by other articles:

• Gendra, E., Colgan, D. F., Meany, B., Konarska, M. M. (2007). A Sequence Motif in the Simian Virus 40 (SV40) Early Core Promoter Affects Alternative Splicing of Transcribed mRNA. J. Biol. Chem. 282: 11648-11657 [Abstract] [Full Text]  
• Kim, H. Y., Barbaro, B. A., Joo, W. S., Prack, A. E., Sreekumar, K. R., Bullock, P. A. (1999). Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication. J. Virol. 73: 7543-7555 [Abstract] [Full Text]