This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bennink, J R Articles by Moss, B Search for Related Content PubMed PubMed Citation Articles by Bennink, J R Articles by Moss, B

Anti-influenza virus cytotoxic T lymphocytes recognize the three viral polymerases and a nonstructural protein: responsiveness to individual viral antigens is major histocompatibility complex controlled.

ABSTRACT

It has recently been shown that antiviral major histocompatibility complex class I-restricted cytotoxic T lymphocytes can recognize proteins that serve as internal viral structural components (influenza A virus nucleoprotein, vesicular stomatitis virus nucleocapsid protein). To further examine the role of internal viral proteins in cytotoxic T-lymphocyte recognition, we constructed recombinant vaccinia viruses containing individual influenza A virus genes encoding three viral polymerases (PB1, PB2, PA) and a protein not incorporated into virions (NS1). We found that cells infected with each of these recombinant vaccinia viruses could be lysed by anti-influenza cytotoxic T lymphocytes. Cytotoxic T-lymphocyte responsiveness to the individual viral antigens varied greatly between mouse strains. By using congenic mouse strains, responsiveness to PB1 and PB2 was found to cosegregate with major histocompatibility complex haplotype. These findings provide further evidence that internal antigens play a critical role in cytotoxic T-lymphocyte recognition of virus-infected cells. Additionally, they suggest that the cytotoxic T-lymphocyte response to viral antigens may often be restricted to only a fraction of the major histocompatibility complex class I repertoire.

This article has been cited by other articles:

• Turner, S. J., Cross, R., Xie, W., Doherty, P. C. (2001). Concurrent Naive and Memory CD8+ T Cell Responses to an Influenza A Virus. J. Immunol. 167: 2753-2758 [Abstract] [Full Text]  
• Tourdot, S., Herath, S., Gould, K. G. (2001). Characterization of a new H-2Dk-restricted epitope prominent in primary influenza A virus infection. J. Gen. Virol. 82: 1749-1755 [Abstract] [Full Text]  
• Belz, G. T., Xie, W., Doherty, P. C. (2001). Diversity of Epitope and Cytokine Profiles for Primary and Secondary Influenza A Virus-Specific CD8+ T Cell Responses. J. Immunol. 166: 4627-4633 [Abstract] [Full Text]  
• Seo, S. H., Webster, R. G. (2001). Cross-Reactive, Cell-Mediated Immunity and Protection of Chickens from Lethal H5N1 Influenza Virus Infection in Hong Kong Poultry Markets. J. Virol. 75: 2516-2525 [Abstract] [Full Text]  
• Belz, G. T., Xie, W., Altman, J. D., Doherty, P. C. (2000). A Previously Unrecognized H-2Db-Restricted Peptide Prominent in the Primary Influenza A Virus-Specific CD8+ T-Cell Response Is Much Less Apparent following Secondary Challenge. J. Virol. 74: 3486-3493 [Abstract] [Full Text]  
• Jameson, J., Cruz, J., Ennis, F. A. (1998). Human Cytotoxic T-Lymphocyte Repertoire to Influenza A Viruses. J. Virol. 72: 8682-8689 [Abstract] [Full Text]  
• Jooss, K., Ertl, H. C. J., Wilson, J. M. (1998). Cytotoxic T-Lymphocyte Target Proteins and Their Major Histocompatibility Complex Class I Restriction in Response to Adenovirus Vectors Delivered to Mouse Liver. J. Virol. 72: 2945-2954 [Abstract] [Full Text]  
• Moss, B (1991). Vaccinia virus: a tool for research and vaccine development. Science 252: 1662-1667 [Abstract]  
• Yewdell, J., Bennink, J. (1989). Brefeldin A specifically inhibits presentation of protein antigens to cytotoxic T lymphocytes. Science 244: 1072-1075 [Abstract]