This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mettenleiter, T C Articles by Lomniczi, B Search for Related Content PubMed PubMed Citation Articles by Mettenleiter, T C Articles by Lomniczi, B

 Previous Article  |  Next Article 

J Virol. 1987 December; 61(12): 4030-4032

Role of a structural glycoprotein of pseudorabies in virus virulence.

Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

ABSTRACT

The virulence of deletion mutants of pseudorabies virus defective in the expression of glycoprotein gI, gp63, or both was tested in 1-day-old chickens and young pigs. In the absence of expression of gI, the virulence of a fully virulent laboratory strain, PrV(Ka), for 1-day-old chickens was reduced approximately fourfold. Inactivation of glycoprotein gp63 appeared also to affect the virulence of PrV(Ka) only slightly, as did inactivation of both gI and gp63. The level of reduction in virulence, however, was considerably more marked in Bartha 43/25aB4, a less virulent virus strain. Inactivation of the expression of gI in Bartha 43/25aB4 reduced virulence for chickens at least 100-fold. The results obtained when the virulence of the mutants for pigs was determined were compatible with those obtained for chickens. These results indicate that gI plays a role in virulence, but that it does so in conjunction with at least one other viral function (a function that is defective in Bartha 43/25aB4).

This article has been cited by other articles:

• Paulus, C., Sollars, P. J., Pickard, G. E., Enquist, L. W. (2006). Transcriptome Signature of Virulent and Attenuated Pseudorabies Virus-Infected Rodent Brain. J. Virol. 80: 1773-1786 [Abstract] [Full Text]  
• Pomeranz, L. E., Reynolds, A. E., Hengartner, C. J. (2005). Molecular Biology of Pseudorabies Virus: Impact on Neurovirology and Veterinary Medicine. Microbiol. Mol. Biol. Rev. 69: 462-500 [Abstract] [Full Text]  
• Fuchs, W., Klupp, B. G., Granzow, H., Hengartner, C., Brack, A., Mundt, A., Enquist, L. W., Mettenleiter, T. C. (2002). Physical Interaction between Envelope Glycoproteins E and M of Pseudorabies Virus and the Major Tegument Protein UL49. J. Virol. 76: 8208-8217 [Abstract] [Full Text]  
• Demmin, G. L., Clase, A. C., Randall, J. A., Enquist, L. W., Banfield, B. W. (2001). Insertions in the gG Gene of Pseudorabies Virus Reduce Expression of the Upstream Us3 Protein and Inhibit Cell-to-Cell Spread of Virus Infection. J. Virol. 75: 10856-10869 [Abstract] [Full Text]  
• Johnson, D. C., Webb, M., Wisner, T. W., Brunetti, C. (2001). Herpes Simplex Virus gE/gI Sorts Nascent Virions to Epithelial Cell Junctions, Promoting Virus Spread. J. Virol. 75: 821-833 [Abstract] [Full Text]  
• Brack, A. R., Klupp, B. G., Granzow, H., Tirabassi, R., Enquist, L. W., Mettenleiter, T. C. (2000). Role of the Cytoplasmic Tail of Pseudorabies Virus Glycoprotein E in Virion Formation. J. Virol. 74: 4004-4016 [Abstract] [Full Text]  
• Tirabassi, R. S., Enquist, L. W. (2000). Role of the Pseudorabies Virus gI Cytoplasmic Domain in Neuroinvasion, Virulence, and Posttranslational N-Linked Glycosylation. J. Virol. 74: 3505-3516 [Abstract] [Full Text]  
• Chowdhury, S. I., Lee, B. J., Ozkul, A., Weiss, M. L. (2000). Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit. J. Virol. 74: 2094-2106 [Abstract] [Full Text]  
• Wisner, T., Brunetti, C., Dingwell, K., Johnson, D. C. (2000). The Extracellular Domain of Herpes Simplex Virus gE Is Sufficient for Accumulation at Cell Junctions but Not for Cell-to-Cell Spread. J. Virol. 74: 2278-2287 [Abstract] [Full Text]  
• Gerdts, V., Beyer, J., Lomniczi, B., Mettenleiter, T. C. (2000). Pseudorabies Virus Expressing Bovine Herpesvirus 1 Glycoprotein B Exhibits Altered Neurotropism and Increased Neurovirulence. J. Virol. 74: 817-827 [Abstract] [Full Text]  
• Brideau, A. D., Card, J. P., Enquist, L. W. (2000). Role of Pseudorabies Virus Us9, a Type II Membrane Protein, in Infection of Tissue Culture Cells and the Rat Nervous System. J. Virol. 74: 834-845 [Abstract] [Full Text]  
• Brack, A. R., Dijkstra, J. M., Granzow, H., Klupp, B. G., Mettenleiter, T. C. (1999). Inhibition of Virion Maturation by Simultaneous Deletion of Glycoproteins E, I, and M of Pseudorabies Virus. J. Virol. 73: 5364-5372 [Abstract] [Full Text]  
• Yang, M., Card, J. P., Tirabassi, R. S., Miselis, R. R., Enquist, L. W. (1999). Retrograde, Transneuronal Spread of Pseudorabies Virus in Defined Neuronal Circuitry of the Rat Brain Is Facilitated by gE Mutations That Reduce Virulence. J. Virol. 73: 4350-4359 [Abstract] [Full Text]  
• Tirabassi, R. S., Enquist, L. W. (1999). Mutation of the YXXL Endocytosis Motif in the Cytoplasmic Tail of Pseudorabies Virus gE. J. Virol. 73: 2717-2728 [Abstract] [Full Text]  
• Tirabassi, R. S., Enquist, L. W. (1998). Role of Envelope Protein gE Endocytosis in the Pseudorabies Virus Life Cycle. J. Virol. 72: 4571-4579 [Abstract] [Full Text]  
• Banfield, B. W., Yap, G. S., Knapp, A. C., Enquist, L. W. (1998). A Chicken Embryo Eye Model for the Analysis of Alphaherpesvirus Neuronal Spread and Virulence. J. Virol. 72: 4580-4588 [Abstract] [Full Text]