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J Virol. 1987 December; 61(12): 3649-3654

ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.

Department of Microbiology, State University of New York, Stony Brook 11794-8261.

ABSTRACT

Simian virus 40 large T antigen initiates DNA replication by binding to the origin of replication. We examined the binding of T antigen to origin regions I, II, and III under conditions designed for efficient in vitro replication functions. We found that 4 mM ATP enhanced the binding of T antigen to regions I and II of the origin DNA by 4- to 20-fold. DNase-footprinting and fragment assays showed that ATP extended the DNase protection domain of T antigen bound to region II by 5 to 10 base pairs at both ends of the core origin of replication. This alteration suggests a change in the conformation of T antigen, bound DNA, or both.

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