This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bellocq, C Articles by Kolakofsky, D Search for Related Content PubMed PubMed Citation Articles by Bellocq, C Articles by Kolakofsky, D

Translational requirement of La Crosse virus S-mRNA synthesis: in vitro studies.

ABSTRACT

The exceptional requirement of La Crosse virus mRNA synthesis for ongoing protein synthesis in vivo was examined in vitro by using purified virions and a reticulocyte lysate. Transcription from the S genome produced two incomplete transcripts (110 and 205 nucleotides [nt]) in the absence of the lysate, whereas S-mRNA (900 nt) was predominantly made when the lysate was present. The addition of drugs which inhibit protein synthesis also inhibited the synthesis of S-mRNA, and in some cases led to the reappearance of the 205-nt RNA. Reconstruction experiments demonstrated that the incomplete transcripts were not the result of rapid and selective degradation of S-mRNA but were due to premature termination of the polymerase at defined sites. The requirement for ongoing protein synthesis for productive transcription in vitro is not at the level of chain initiation but for elongation of the nascent RNA beyond these sites.

This article has been cited by other articles:

• Ikegami, T., Won, S., Peters, C. J., Makino, S. (2007). Characterization of Rift Valley Fever Virus Transcriptional Terminations. J. Virol. 81: 8421-8438 [Abstract] [Full Text]  
• Barr, J. N. (2007). Bunyavirus mRNA synthesis is coupled to translation to prevent premature transcription termination. RNA 13: 731-736 [Abstract] [Full Text]  
• van Knippenberg, I., Goldbach, R., Kormelink, R. (2004). In vitro transcription of Tomato spotted wilt virus is independent of translation. J. Gen. Virol. 85: 1335-1338 [Abstract] [Full Text]  
• Bridgen, A., Weber, F., Fazakerley, J. K., Elliott, R. M. (2001). Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis. Proc. Natl. Acad. Sci. USA 98: 664-669 [Abstract] [Full Text]  
• Rust, R. S., Thompson, W. H., Matthews, C. G., Beaty, B. J., Chun, R. W.M. (1999). Topical Review: La Crosse and Other Forms of California Encephalitis. J Child Neurol 14: 1-14 [Abstract]