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Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic Peptide

¹ Department of Molecular Biology, Agricultural University, De Dreyen 11, 6703 BC Wageningen, The Netherlands

ABSTRACT

A peptide was synthesized that corresponded to a sequence in the cowpea mosaic virus bottom-component RNA-encoded 200-kilodalton polyprotein showing homology to the picornaviral 3C proteases. By injecting a rabbit with this peptide, antibodies were obtained that allowed the detection of a novel viral protein derived from the 200-kilodalton polyprotein. This protein, which had a size of 24 kilodaltons was found in both infected cowpea leaves and cowpea protoplasts.

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