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J Virol. 1970 December; 6(6): 834-840
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

LPP-1 Infection of the Blue-Green Alga Plectonema boryanum

II. Viral Deoxyribonucleic Acid Synthesis and Host Deoxyribonucleic Acid Breakdown

Department of Biophysics, University of Chicago, Chicago, Illinois 60637

ABSTRACT

Host and viral deoxyribonucleic acid (DNA) metabolism in LPP-1-infected Plectonema boryanum was studied by equilibrium centrifugation in CsCl gradients. Approximately 50% of the host DNA is degraded to acid-soluble material between 3 and 7 hr after infection. Most of the acid-soluble product is reincorporated into viral DNA. Incorporation of exogenous ³H-adenine into viral DNA can be detected very early after infection (within the first 2 hr), but the bulk of viral DNA synthesis occurs between 6 and 8 hr. Both the breakdown of host DNA and the synthesis of viral DNA require protein synthesis during the first few hours of infection.

¹ Present address: Section of Genetics, Physiology and Development, Cornell University, Ithaca, N.Y.