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Incorporation of Deoxyribonucleic Acid Precursors by T4 Deoxyribonucleic Acid-Protein Complexes Retained on Glass Fiber Filters

Department of Medical Genetics and Graduate Group of Molecular Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19146

ABSTRACT

Bacteriophage T4 deoxyribonucleic acid (DNA)-protein complexes were retained preferentially on glass fiber filters. DNA polymerase activity in the complex was detected through the incorporation of ³H-labeled DNA precursors. The primer-product DNA hybridized with both phage and Escherichia coli DNA. Density labeling experiments showed that about 30% of incorporated ³H-deoxyadenosine triphosphate was found in DNA which hybridized with phage DNA; this DNA was found to be covalently attached to the primer DNA.

¹ Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass.