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J Virol. 1970 September; 6(3): 353-362
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Conversion of T4 Gene 46 Mutant Deoxyribonucleic Acid into Nonviable Bacteriophage Particles

Channa Shalitin and Simona Kahana1

Department of Chemistry, Israel Institute of Technology, Haifa, Israel

ABSTRACT

The T4 amber mutant N130 in gene 46 produces, upon infection of a nonpermissive host, about 10 times more phage equivalent of deoxyribonucleic acid (DNA) than viable phage particles. Intracellular DNA is shorter than its mature phage counterpart and is converted into nonviable, light phage particles (287S) with about half of the normal DNA content, incapable either of adsorbing to or killing host bacteria. This DNA is highly unstable and breaks down, upon extraction, into subunits one-tenth the normal length of T4 DNA. Electron micrographs showed that the nonviable particles consist of normal-sized capsids of various degrees of fullness. Abnormal tail structures (naked cores with the sheaths missing) were also observed. Under conditions of arrested DNA synthesis, late messenger ribonucleic acid is produced, but some species are rare or missing, resulting in uneven transcription of the late genes. Our findings indicate that continuous DNA replication is necessary for normal transcription in T4.

FOOTNOTES

1 Parts of this study were conducted by S. Kahana in partial fulfillment of the requirements of M.S. degree at the Department of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.

J Virol. 1970 September; 6(3): 353-362
Copyright © 1970 American Society for Microbiology. All Rights Reserved.





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