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J Virol. 1970 September; 6(3): 340-343
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Genetic Mapping of a Defective Bacteriophage on the Chromosome of Bacillus subtilis 168

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461

ABSTRACT

A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to metC. The link-age relationship between xtl and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being argO 1 metA metC xtl.

¹ Present address: Department of Microbiology, Mt. Sinai School of Medicine, New York, N.Y. 10029.