This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Norrby, E. Articles by Örvell, C. Search for Related Content PubMed PubMed Citation Articles by Norrby, E. Articles by Örvell, C.

Morphogenesis of Respiratory Syncytial Virus in a Green Monkey Kidney Cell Line (Vero)

Department of Virology, Karolinska Institutet, School of Medicine, Stockholm, Sweden

ABSTRACT

The structure and morphogenesis of respiratory syncytial (RS) virus particles in a green monkey kidney cell line (Vero) were examined. Infected cells contained dense intracytoplasmic inclusions composed of filamentous structures. In places where inclusion material was associated with membranes, structural modifications were induced. There was a thickening of the membrane and an addition of projections 12 to 15 nm in length. The same changes were most frequently observed after association of isolated filamentous structures with the cytoplasmic membrane. The budding-off process was clearly visualized. The diameter of mature virus particles varied between 90 and 130 nm and that of the internal component varied between 11 and 15 nm. The similarities between ultrastructural features of cells infected with RS virus and pneumonia virus of mice are pointed out. It is proposed that these two viruses should be classified together in a third subgroup of myxoviruses.

This article has been cited by other articles:

• Utley, T. J., Ducharme, N. A., Varthakavi, V., Shepherd, B. E., Santangelo, P. J., Lindquist, M. E., Goldenring, J. R., Crowe, J. E. Jr. (2008). Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2. Proc. Natl. Acad. Sci. USA 105: 10209-10214 [Abstract] [Full Text]  
• Santangelo, P. J., Bao, G. (2007). Dynamics of filamentous viral RNPs prior to egress. Nucleic Acids Res 35: 3602-3611 [Abstract] [Full Text]  
• Santangelo, P., Nitin, N., LaConte, L., Woolums, A., Bao, G. (2006). Live-Cell Characterization and Analysis of a Clinical Isolate of Bovine Respiratory Syncytial Virus, Using Molecular Beacons. J. Virol. 80: 682-688 [Abstract] [Full Text]  
• Kvellestad, A., Dannevig, B. H., Falk, K. (2003). Isolation and partial characterization of a novel paramyxovirus from the gills of diseased seawater-reared Atlantic salmon (Salmo salar L.). J. Gen. Virol. 84: 2179-2189 [Abstract] [Full Text]