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J Virol. 1986 July; 59(1): 185-188

Human cytomegalovirus virion-associated protein with kinase activity.

W J Britt and D Auger

ABSTRACT

Protein kinase activity was detected in immunoprecipitates of human cytomegalovirus virions and infected cells by using a monoclonal antibody directed against an abundant 68,000-dalton virion structural protein. Purification of this protein by electrophoresis confirmed that the kinase activity was associated with this protein. The kinase activity was dependent on divalent cations (Mg2+, Mn2+) and cyclic nucleotide independent and exhibited optimal activity at pH 7 to 8. The kinase phosphorylated threonine and serine but not tyrosine.

J Virol. 1986 July; 59(1): 185-188




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