This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsukura, T Articles by Yoshiike, K Search for Related Content PubMed PubMed Citation Articles by Matsukura, T Articles by Yoshiike, K

 Previous Article  |  Next Article 

J Virol. 1986 June; 58(3): 979-982

Cloning of monomeric human papillomavirus type 16 DNA integrated within cell DNA from a cervical carcinoma.

ABSTRACT

We have molecularly cloned and characterized monomeric human papillomavirus type 16 DNA with flanking cell DNA sequences from a cervical carcinoma. Determination of nucleotide sequence around the junctions of human papillomavirus and cell DNAs revealed that at the site of integration within cell DNA the cloned viral DNA had a deletion between nucleotides 1,284 and 4,471 (numbering system from K. Seedorf, G. Krämmer, M. Dürst, S. Suhai, and W. G. Röwekamp, Virology 145:181-185, 1985), which includes the greater part of E1 gene and the entire E2 gene. In the remaining part of the E1 gene, three guanines were found at the location where two guanines at nucleotides 1,137 and 1,138 have been recorded. This additional guanine shifted the reading frame and erased an interruption in the E1 gene described by Seedorf et al. The data strongly suggest that, like other papillomaviruses, human papillomavirus type 16 has an uninterrupted E1 gene.

This article has been cited by other articles:

• Bechtold, V., Beard, P., Raj, K. (2003). Human Papillomavirus Type 16 E2 Protein Has No Effect on Transcription from Episomal Viral DNA. J. Virol. 77: 2021-2028 [Abstract] [Full Text]  
• Lee, D., Lee, B., Kim, J., Kim, D. W., Choe, J. (2000). cAMP Response Element-binding Protein-binding Protein Binds to Human Papillomavirus E2 Protein and Activates E2-dependent Transcription. J. Biol. Chem. 275: 7045-7051 [Abstract] [Full Text]  
• Zou, N., Liu, J.-S., Kuo, S.-R., Broker, T. R., Chow, L. T. (1998). The Carboxyl-Terminal Region of the Human Papillomavirus Type 16 E1 Protein Determines E2 Protein Specificity during DNA Replication. J. Virol. 72: 3436-3441 [Abstract] [Full Text]  
• Suzuk, L., Noffsinger, A. E., Ali, M., Fenoglio-Preiser, C. M. (1997). A High Frequency of Human Papilloma Virus (HPV) DNA Type 16 in Biopsies of Cervical Squamous Cell Neoplasia of Uygur Women. INT J SURG PATHOL 4: 219-225 [Abstract]  
• Dyson, N, Howley, P., Munger, K, Harlow, E (1989). The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243: 934-937 [Abstract]