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J Virol. 1986 April; 58(1): 223-224

Location of the Bacillus subtilis temperate bacteriophage phi 105 attP attachment site.

D M Ellis and D H Dean

ABSTRACT

Chromosomal DNAs of lysogens of phi 105 and phi 105 DI:1t were digested with restriction enzymes EcoRI and HpaI and were probed with nick-translated mature phi 105 DNA. Altered bacteriophage-specific bands in the lysogens were detected, indicating that the phage integrates into the host chromosome at a single site, probably via a Campbell-type circular intermediate. The phage attachment site is centrally located in the phage genome and lies between the phage immunity region and the nonessential deletable region of phi 105.

J Virol. 1986 April; 58(1): 223-224






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